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Volumn 284, Issue 5413, 1999, Pages 505-507

Regulation of the mammalian pineal by non-rod, non-cone, ocular photoreceptors

Author keywords

[No Author keywords available]

Indexed keywords

MELATONIN;

EID: 0033574769     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5413.505     Document Type: Article
Times cited : (458)

References (30)
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    • The cl transgene was previously characterized in C57BL/6 mice (12). However, because this strain of mice does not produce pineal melatonin [S. Ebihara, T. Marks, D. J. Hudson, M. Menaker. Science 231, 491 (1986)], we introduced this transgene into the C3H/ He strain by at least nine generations of backcrossing.
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    • Ebihara, S.1    Marks, T.2    Hudson, D.J.3    Menaker, M.4
  • 14
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    • Our reference to the cl transgene corresponds to the h.red DT-A construct in this paper
    • E. Soucy, Y. Wang, S. Nirenberg, J. Nathans, M. Meister, Neuron 21, 481 (1998). Our reference to the cl transgene corresponds to the h.red DT-A construct in this paper.
    • (1998) Neuron , vol.21 , pp. 481
    • Soucy, E.1    Wang, Y.2    Nirenberg, S.3    Nathans, J.4    Meister, M.5
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    • note
    • max = 509 nm, half band width 10 nm) at zeitgeber time 20-21. The melatonin content of pineal homogenates was assessed by ra dioimmunoassay as described (9).
  • 18
    • 0024990758 scopus 로고
    • C Bowes ef al., Nature 347, 677 (1990).
    • (1990) Nature , vol.347 , pp. 677
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    • 1:20,000 dilution
    • We used polyclonal antisera (12) to the human blue cone pigment (used at 1:8000 dilution) and human red-green pigments (1:8000 dilution) and a monoclonal antibody to rat rod opsin [D. Hicks and R. S. Molday, Exp. Eye Res. 42, 55 (1986); 1:20,000 dilution].
    • (1986) Exp. Eye Res. , vol.42 , pp. 55
    • Hicks, D.1    Molday, R.S.2
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    • M. Freedman et al., Science 284, 502 (1999).
    • (1999) Science , vol.284 , pp. 502
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    • note
    • We thank J. Nathans and Y. Wang (Johns Hopkins University) for donation of the original C57BL/6 cl mouse colony and the polyclonal antisera to cones and D. Hicks for the monoclonal antisera to rods. This work was supported by research grants from UK Biotechnology and Biological Sciences Research Council and European Union BioMed 2 program.


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