The RRM protein nonA from Drosophila forms a complex with the RRM proteins Hrb87F and s5 and the Zn finger protein PEP on hnRNA

Experimental Cell Research

Volumn 253, Issue 2, 1999, Pages 573-586

  Reim, Ingolf ^a   Mattow, Jens ^a,b   Saumweber, Harald ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

Author keywords

Polytene chromosomes puff specific proteins; RNA binding; Transcription

Indexed keywords

HISTONE H3; HISTONE H4; MONOCLONAL ANTIBODY; NUCLEAR PROTEIN; RIBONUCLEASE; RNA; RNA BINDING PROTEIN;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DROSOPHILA; IMMUNOPRECIPITATION; NONHUMAN; POLYTENE CHROMOSOME; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN RNA BINDING;

DROSOPHILA;

EID: 0033572766     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1999.4647     Document Type: Article

References (62)

1. Matunis M. J., Matunis E. L., Dreyfuss G. Isolation of hnRNP complexes from Drosophila melanogaster. J. Cell Biol. 116:1992;245-254.
2. Matunis E. L., Matunis M. J., Dreyfuss G. Characterization of the major hnRNP proteins from Drosophila melanogaster. J. Cell Biol. 116:1992;257-269.
3. Swanson M. Functions of nuclear pre-mRNA/mRNA binding proteins. Lamond A. I. Pre-mRNA Processing. 1995;R. G. Landes, Austin/Springer Verlag, New York.
4. Krämer A. The biochemistry of pre-mRNA splicing. Lamond A. I. Pre-mRNA Processing. 1995;R. G. Landes, Austin/Springer Verlag, New York.
5. Keller W. 3′-end cleavage and polyadenylation of nuclear messenger RNA precursors. Lamond A. I. Pre-mRNA Processing. 1995;R. G. Landes, Austin/Springer Verlag, New York.
6. Siomi H., Dreyfuss G. RNA-binding proteins as regulators of gene expression. Curr. Opin. Genet. Dev. 7:1997;345-353.
7. Nagai K., Oubridge C., Jessen T. H., Li J., Evans P. R. Crystal structure of the RNA binding domain of the U1 small nuclear ribonucleoprotein A. Nature. 348:1990;515-520.
8. Nagai K., Oubridge C., Ito N., Avis J., Evans P. R. The RNP domain: A sequence-specific RNA-binding domain involved in processing and transport of RNA. Trends Biochem. Sci. 20:1995;235-240.
9. Oubridge C., Ito N., Evans P. R., Teo C. H., Nagai K. Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin. Nature. 372:1994;432-438.
10. Hoffman D. E., Query C. C., Golden B. L., White S. W., Keene J. D. RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins. Proc. Natl. Acad. Sci. USA. 88:1981;2495-2499.
11. Chase J. W., Williams K. R. Single-stranded DNA binding proteins required for DNA replication. Annu. Rev. Biochem. 55:1986;103-136.
12. Kiledjian M., Dreyfuss G. Primary structure and binding activity of the hnRNP U protein: Binding RNA through RGG box. EMBO J. 11:1992;2655-2664.
13. Siomi H., Matunis M. J., Michael W. M., Dreyfuss G. The pre-mRNA binding K protein contains a novel evolutionarily conserved motif. Nucleic Acids. Res. 21:1993;1193-1198.
14. Miller J., McLachlan A. D., Klug A. Repetitive zinc binding domains in the protein transcription factor TFIII from Xenopus oocytes. EMBO J. 4:1985;1609-1614.
15. Darby M. K., Joho K. E. Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S gene. Mol. Cell. Biol. 12:1992;3155-3164.
16. Bennet M., Reed R. Correspondence between a mammalian spliceosome component and an essential yeast splicing factor. Science. 262:1993;105-108.
17. Chiara M. D., Champion-Arnaud P., Bouvoli M., Nadal-Ginard B., Reed R. Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP61 and SAP114. Proc. Natl. Acad. Sci. USA. 91:1994;6403-6407.
18. Amero S. A., Elgin S. C. R., Beyer A. L. A unique zinc finger protein is associated preferentially with active ecdysone responsive loci in Drosophila. Genes Dev. 5:1991;188-200.
19. Amero S. A., Matunis M. J., Matunis E. L., Hockensmith J. W., Raychaudhuri G., Beyer A. L. A unique ribonucleoprotein complex assembles preferentially on ecdysone-responsive sites in Drosophila melanogaster. Mol. Cell. Biol. 13:1993;5323-5330.
20. Besser H. v., Schnabel P., Wieland C., Fritz E., Stanewsky R., Saumweber H. The puff-specific protein Bj6, encoded by the gene no-on transient A, shows homology to RNA-binding proteins. Chromosoma. 100:1990;37-47.
21. Frasch M., Saumweber H. Two proteins from Drosophila nuclei are bound to chromatin and are detected in a series of puffs on polytene chromosomes. Chromosoma. 97:1989;272-281.
22. Saumweber H., Korge G., Frasch M. Two puff-specific proteins bind within the 2.5 k.b. upstream region of the Drosophila melanogaster Sgs-4 gene. Chromosoma. 99:1990;52-60.
23. Reim I., Stanewsky R., Saumweber H. The puff-specific RRM-protein NonA is a single-stranded nucleic acid binding protein. Chromosoma. 108:1999;162-172.
24. Rendahl K. G., Jones K. R., Kulkarni S. J., Bagully S. H., Hall J. C. The dissonance mutation at the no-on transient A locus of D. melanogaster: Genetic control of courtship song and visual behaviors by a protein with putative RNA-binding motifs. J. Neurosci. 12:1992;390-407.
25. Stanewsky R., Rendahl K. G., Dill M., Saumweber H. Genetic and molecular analysis of the X-chromosomal region 14B17-14C4 in Drosophila melanogaster: Loss of function in nonA, a nuclear protein common to many cell types, results in specific physiological and behavioral defects. Genetics. 135:1993;419-442.
26. Stanewsky R., Fry T. A., Reim I., Saumweber H., Hall J. C. Bioassaying putative RNA-binding motifs in a protein encoded by a gene that influences courtship and visually mediated behavior in Drosophila: In vitro mutagenesis of nonA. Genetics. 143:1996;259-275.
27. Rendahl K. G., Gaukhshteyn N., Wheeler D. A., Fry T. A., Hall J. C. Defects in courtship and vision caused by amino acid substitutions in a putative RNA-binding protein encoded by the no-on-transient A (nonA) gene of Drosophila. J. Neurosci. 16:1996;1511-1521.
28. Risau W., Symmons P., Saumweber H., Frasch M. Nonpackaging and packaging proteins of hnRNA in Drosophila melanogaster. Cell. 33:1983;529-541.
29. Saumweber H., Symmons P., Kabisch R., Will H., Bonhoeffer F. Monoclonal antibodies against chromosomal proteins of Drosophila melanogaster. Chromosoma. 80:1980;253-275.
30. Reim, I. 1996. Unpublished data.
31. Krämer A., Haars R., Kabisch R., Will H., Bautz F. A., Bautz E. K. F. Monoclonal antibody against RNA polymerase II of Drosophila melanogaster. EMBO J. 2:1980;395-402.
32. Martin R. G., Ames B. N. A method for determining the sedimentation behavior of enzymes: Application to protein mixtures. J. Biol. Chem. 236:1961;1372-1379.
33. Otto A., Thiede B., Müller E.-C., Scheler C., Wittmann-Liebold B., Jungbluth P. Identification of human myocardial proteins separated by two-dimensional electrophoresis using an effective sample preparation for mass spectrometry. Electrophoresis. 17:1996;1643-1650.
34. Scheler C., Lamer S., Pan Z., Li X.-P., Salnikow J., Jungblut P. Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Electrophoresis. 19:1998;918-927.
35. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
36. Kejzlarova-Lepesent J., Brock H. W., Moreau J., Dubertret M. L., Billault A., Lepesant J. A. A complete and truncated U1 snRNA gene of Drosophila melanogaster are found as inverted repeats at region 82E of the polytene chromosomes. Nucleic Acids. Res. 12:1984;8835-8846.
37. Alonso A., Beck E., Jorcano J. L., Hovemann B. Divergence of U2 snRNA sequences in the genome of D. melanogaster. Nucleic Acids Res. 12:1984;9543-9550.
38. Renkawitz-Pohl R., Glätzer K. H., Kunz W. Characterization of cloned ribosomal DNA from Drosophila hydei. Nucleic Acids Res. 8:1980;4593-4611.
39. O'Connel P., Rosbash M. Sequence, structure and codon preference of the Drosophila ribosomal protein 49 gene. Nucleic Acids Res. 12:1984;5495-5513.
40. Frasch M. The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of chromatin condensation RCC1. EMBO J. 10:1991;1225-1236.
41. Wieland C., Mann S., Besser H. v., Saumweber H. The Drosophila nuclear protein Bx42, which is found in many puffs on polytene chromosomes, is highly charged. Chromosoma. 101:1992;517-525.
42. 0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene. J. Cell Biol. 106:1988;585-596.
43. Heiermann R., Pongs O. In vitro transcription with extracts of nuclei of Drosophila. Nucleic Acids Res. 13:1985;2709-2730.
44. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
45. Hovemann, B. T. 1996. Personal communication.
46. Haynes S. R., Johnson G., Raychaudhuri G., Beyer A. L. The Drosophila Hrb87F gene encodes a new member of the A and B hnRNP protein group. Nucleic Acids Res. 19:1991;25-32.
47. Hovemann B. T., Dessen E., Mechler H., Mack E. Drosophila snRNP associated protein P11 which specifically binds to heat shock puff 93D reveals strong homology with hnRNP core protein A1. Nucleic Acids Res. 19:1991;4909-4914.
48. Glätzer, K. H. 1998. Personal communication.
49. Hamann S., Strätling W. H. Specific binding of Drosophila nuclear protein PEP (protein on ecdysone puffs) to hsp70 DNA and RNA. Nucleic Acids Res. 26:1998;4108-4115.
50. Patton J. G., Poro E. B., Galceran J., Tempst P., Nadal-Ginard B. Cloning and characterization of PSF, a novel pre-mRNA splicing factor. Genes Dev. 7:1993;393-406.
51. nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NonA/Bj6. Nucleic Acids Res. 21:1993;4085-4092.
52. diss. Mol. Cell. Biol. 13:1993;5593-5603.
53. Reuter G., Giarre M., Farah J., Gausz J., Spierer A., Spierer P. Dependence of position-effect variegation in Drosophila on dose of a gene encoding an unusual zinc-finger protein. Nature. 344:1990;219-223.
54. Fasano L., Röder L., Coré N., Alexandre E., Vola C., Jacq B., Kerridge S. The gene teashirt is required for the development of Drosophila embryonic trunk segments and encodes a protein with widely spaced zinc finger motifs. Cell. 64:1991;63-79.
55. Mayeda A., Krainer A. R. Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2. Cell. 68:1992;365-375.
56. Zu K., Sikes M. L., Beyer A. L. Separable roles in vivo for the two RNA binding domains of a Drosophila A1-hnRNP homolog. RNA. 4:1998;1585-1598.
57. Schuldt C., Kloetzel P. M., Bautz E. K. F. Molecular organization of RNP complexes containing P11 antigen in heat-shocked and non-heat-shocked Drosophila cells. Eur. J. Biochem. 181:1989;135-142.
58. Li B., Fields C. Identification of mutations in p53 that affect its binding to SV40 large T antigen by using the yeast two-hybrid system. FASEB J. 7:1993;957-963.
59. Saumweber, H. Unpublished data.
60. Pinol-Roma S., Dreyfuss G. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. Nature. 355:1992;730-732.
61. Sharp P. A., Marcinisk R. A. HIV TAR: An RNA enhancer. Cell. 59:1998;29-230.
62. Huang D., Sorensen K., Bass K., Amero S. A model for PEP, the protein on ecdysone puffs, in higher level chromosome structure. A. Conf. Dros. Res. 39:1998;768B.