Reductive half-reaction of the H172Q mutant of trimethylamine dehydrogenase: Evidence against a carbanion mechanism and assignment of kinetically influential ionizations in the enzyme-substrate complex

Biochemical Journal

Volumn 341, Issue 2, 1999, Pages 307-314

  Basran, Jaswir ^a   Sutcliffe, Michael J ^a   Hille, Russ ^b   Scrutton, Nigel S ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b OHIO STATE UNIVERSITY   (United States)

Author keywords

Amine oxidation; Electron transfer; Flavoprotein

Indexed keywords

DEUTERIUM; HISTIDINE; MUTANT PROTEIN; OXIDOREDUCTASE; PROTON; QUERCETIN; TRIMETHYLAMINE;

ARTICLE; CONTROLLED STUDY; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; IONIZATION; NONHUMAN; PH; PRIORITY JOURNAL; SPECTROSCOPY;

ENZYME ACTIVATION; GRAM-NEGATIVE AEROBIC BACTERIA; HISTIDINE; KINETICS; OXIDOREDUCTASES, N-DEMETHYLATING; POINT MUTATION; SUBSTRATE SPECIFICITY;

EID: 0033565414     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3410307     Document Type: Article

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