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0030028901
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PCR analysis for the Y chromosome was done on DNA extracted from transplanted and nontransplanted female animals with primers for the sry gene of the Y chromosome (J. An, V. N. Beauchemin, J. Albanese, A. K. Sullivan, J. Androl. 18, 289 (1997)].
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0345586888
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note
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In situ hybridization of the sry region of the Y chromosome was performed with digoxigenin-labeled DNA probes (Boehringer Mannheim) hybridized to frozen liver sections.
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-
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13
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0345586887
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note
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+ oval cells expressing the Y marker was calculated from in situ hybridization cytospin preparation from four different animals.
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-
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14
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0345586885
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For immunocytochemistry, tissue was placed in optimum cutting temperature compound (O.C.T.) and cold 2-methylbutane and processed for frozen sections. DPPIV activity was detected with the cytochemical method of Z. Lojdo [Histochemistry 57, 107 (1979)].
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I. M. Arias et al., Eds. Raven, New York, ed. 3, chap. 78
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N. Fausto, in The Liver: Biology and Pathobiology, I. M. Arias et al., Eds. (Raven, New York, ed. 3, 1994), chap. 78.
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Fausto, N.1
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19
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0345586886
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note
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4 oval cell induction protocol. A portion of the cells in the periportal region were positive for both L21-6-FITC and OC-2-Texas Red (an oval cell and mature ductal cell marker) by immunofluorescence.
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21
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Asahara, T.1
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25
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0344292610
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note
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- female F-344 rats; and R. Farris and D. Hixson for antibodies.
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