Muscle regeneration by bone marrow-derived myogenic progenitors

Science

Volumn 279, Issue 5356, 1998, Pages 1528-1530

  Ferrari, Giuliana ^a   Cusella De Angelis, Gabriella ^b   Coletta, Marcello ^c   Paolucci, Egle ^a   Stornaiuolo, Anna ^a   Cossu, Giulio ^c   Mavilio, Fulvio ^a  

^a SAN RAFFAELE SCIENTIFIC INSTITUTE   (Italy)

^b UNIVERSITY OF PAVIA   (Italy)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BONE MARROW TRANSPLANTATION; CELL DIFFERENTIATION; CELL GROWTH; CELL MIGRATION; HUMAN; HUMAN CELL; HUMAN TISSUE; MUSCLE CELL; MUSCLE REGENERATION; MUSCULAR DYSTROPHY; PRIORITY JOURNAL; SKELETAL MUSCLE;

ANIMALS; BETA-GALACTOSIDASE; BONE MARROW CELLS; BONE MARROW TRANSPLANTATION; CELL DIFFERENTIATION; CELL MOVEMENT; GENE THERAPY; HUMANS; MICE; MICE, SCID; MICE, TRANSGENIC; MUSCLE FIBERS; MUSCLE, SKELETAL; MUSCULAR DYSTROPHIES; REGENERATION; STEM CELLS; STROMAL CELLS;

EID: 0032489651     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5356.1528     Document Type: Article

References (27)

1. D. R. Campion, Int. Rev. Cytol. 87, 225 (1984); H. M. Blau, J. Dhawan, G. K. Pavlath, Trends Genet. 9, 269 (1993).
2. D. R. Campion, Int. Rev. Cytol. 87, 225 (1984); H. M. Blau, J. Dhawan, G. K. Pavlath, Trends Genet. 9, 269 (1993).
3. E. Schultz and B. H. Lipton, Mech, Ageing Dev. 13, 105 (1980).
4. A. Emery, Duchenne Muscular Dystrophy (Oxford Univ. Press. New York, 1993), vol. 1.
5. M. D. Grounds, K. L. Garrett, M. C. Lai, W. E. Wright, M. W. Beilharz, Cell Tissue Res. 267, 99 (1992).
6. G. Cossu, Histol. Histopathol. 12, 755 (1997).
7. R. F. Pereira et al., Proc. Natl. Acad. Sci. U.S.A. 92, 4857 (1995).
8. S. Wakitani, T. Saito, A. I. Caplan, Muscle Nerve 18, 1417 (1995).
9. D. J. Prockop, Science 276, 71 (1997).
10. The scid/bg mouse strain combines the characteristics of scid (severe combined immunodeficiency) animals, which lack functional B and T cells, with those of beige (bg) animals, which have intrinsically low natural killer (NK) cell activity. The scid/bg mice show a more stable SCID phenotype and are, in general, better recipients for allograft transplantation [A. M. Krensky, Nature Biotechnol. 15, 720 (1997)]. Seven-to 9-week-old C.B-17 scid/bg mice were obtained from Charles River (Calco, Italy) and maintained under specific pathogen-free conditions. Mice were anesthetized and regeneration was induced in TA muscles by injection of 25 μl of 1 mM cardiotoxin (Latoxan, Rosans, France). Animal experimentation protocols were approved by the HSR Institutional Animal Care and Use Committee.
11. 6 cells in 25 μl of phosphate-buffered saline (PBS)]into regenerating TA muscles of scid/bg mice 24 hours after cardiotoxin injection.
12. R. Kelly, S. Alonso, S. Tajbakhsh, G. Cossu, M. Buckingham, J. Cell Biol. 129, 383 (1995).
13. S. Tajbakhsh et al., Neuron 13, 813 (1994); G. Salvatori et al., J. Cell Sci. 108, 2733 (1995).
14. S. Tajbakhsh et al., Neuron 13, 813 (1994); G. Salvatori et al., J. Cell Sci. 108, 2733 (1995).
15. 5 cells in 25 μl) into regenerating TA muscles of scid/bg mice 24 hours after cardiotoxin injection.
16. TA muscles were removed, fixed in 4% paraformaldehyde, embedded in omithyne carbamoyl transferase, frozen in liquid nitrogen-cooled isopentane, and cut on a cryostat into 15- to 20-μm serial sections. β-Gal activity was assayed in whole-mount preparations or in cryostat sections by X-Gal staining (10).
17. + nuclei divided by the total number of injected cells) ranged from 0.5 to 8.5%.
18. 5) cells were collected, resuspended in 25 μ\ of PBS, and injected separately into regenerating TA muscles of scid/bg mice 24 hours after cardiotoxin injection.
19. b) donors were injected into the tail vein of the irradiated animals. Animals were maintained under specific pathogen-free conditions throughout the procedure. The scid/bg mutants were chosen as a model for BM transplantation because of a lack of a suitable co-isogenic host and because (i) immunodeficient mice are fully reconstituted after low-dose irradiation [G. M. Fulop and R. A. Phillips, J. Immunol. 136, 4438 (1986)]; (ii) graftversus-host disease is not observed in scid mice, avoiding the necessity of any pretransplant manipulation, such as T cell depletion, of BM cells; and (iii) bg mice are severely deficient in the NK cell component, thus overcoming the problem of NK cell-mediated allograft rejection [W. J. Murphy, V. Kumar, M. Bennett, J. Exp. Med. 165, 1212 (1987)].
20. b) donors were injected into the tail vein of the irradiated animals. Animals were maintained under specific pathogen-free conditions throughout the procedure. The scid/bg mutants were chosen as a model for BM transplantation because of a lack of a suitable co-isogenic host and because (i) immunodeficient mice are fully reconstituted after low-dose irradiation [G. M. Fulop and R. A. Phillips, J. Immunol. 136, 4438 (1986)]; (ii) graftversus-host disease is not observed in scid mice, avoiding the necessity of any pretransplant manipulation, such as T cell depletion, of BM cells; and (iii) bg mice are severely deficient in the NK cell component, thus overcoming the problem of NK cell-mediated allograft rejection [W. J. Murphy, V. Kumar, M. Bennett, J. Exp. Med. 165, 1212 (1987)].
21. Two mice did not survive radiation treatment, and one animal was killed after 4 weeks to monitor engraftment.
22. Analysis of the size and cellularity of the thymus and spleen, usually reduced to one-tenth of normal in scid/bg animals, analysis of Giemsa-stained cyto-centrifuged cell preparations, and flow cytometric analysis of BM, thymus, and spleen cells were used as additional criteria to confirm engraftment in central and peripheral hematopoietic organs.
23. + nuclei. Positive muscles contained 50 to 100 blue nuclei, clustered in < 1 % of the regenerated fibers.
24. T. A. Partridge and K. E. Davies, Br. Med. Bull. 51, 123 (1995); A. D. Miller, Nature Med. 3, 278 (1997).
25. T. A. Partridge and K. E. Davies, Br. Med. Bull. 51, 123 (1995); A. D. Miller, Nature Med. 3, 278 (1997).
26. T. A. Partridge, Muscle Nerve 14, 197 (1991).
27. Supported by grants from the Italian Telethon Foundation and the European Union Biotechnology Program. We thank D. Sartori, R. Parma, and G. Torriani for technical assistance; R. Kelly for MLC3F-nlacZ-E transgenic mice; and C. Bordignon and M. Buckingham for critical discussion and continuous support.