Catalytic single-chain antibodies possessing β-lactamase activity selected from a phage displayed combinatorial library using a mechanism-based inhibitor

Tetrahedron Letters

Volumn 40, Issue 46, 1999, Pages 8063-8066

  Tanaka, Fujie ^a   Almer, Helena ^a   Lerner, Richard A ^a   Barbas III, Carlos F ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Antibodies; Azetidinones; Catalysis; Phage display

Indexed keywords

AMPICILLIN; AZETIDINONE DERIVATIVE; BETA LACTAMASE; CATALYTIC ANTIBODY;

ANIMAL TISSUE; ARTICLE; CATALYSIS; DRUG HYDROLYSIS; ESCHERICHIA COLI; MOUSE; NONHUMAN; PHAGE DISPLAY;

ANIMALIA; ESCHERICHIA COLI; FELIS CATUS;

EID: 0033550282     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(99)01695-0     Document Type: Article

References (21)

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15. 2 by insertion of the zeocin resistance gene into the middle of the β-lactamase coding region. The zeocin resistance gene was taken from pPICZαA vector (Invitrogen) by PCR.
16. pZErH6HA vector was constructed from pZErO-1 (Invitrogen).
17. Barbas III, C. F.; Wagner J. Methods: A Companion to Methods in Enzymology 1995, 8, 94.
18. The scFv protein concentration was determined by Bradford analysis.
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