Crystal structure of nerve growth factor in complex with the ligand- binding domain of the TrkA receptor

Nature

Volumn 401, Issue 6749, 1999, Pages 184-188

  Wlesmann, Christian ^a   Ultsch, Mark H ^a   Bass, Steven H ^a   De Vos, Abraham M ^a  

^a GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE RECEPTOR; NERVE GROWTH FACTOR; NERVE GROWTH FACTOR RECEPTOR; NEUROTROPHIN; TYROSINE KINASE RECEPTOR;

ARTICLE; CELL DIFFERENTIATION; CELL SURVIVAL; CRYSTAL STRUCTURE; HUMAN; MOUSE; NERVE CELL NECROSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN PROTEIN INTERACTION;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; HUMANS; LIGANDS; MACROMOLECULAR SUBSTANCES; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NERVE GROWTH FACTORS; PROTEIN CONFORMATION; PROTO-ONCOGENE PROTEINS; RECEPTOR PROTEIN-TYROSINE KINASES; RECEPTOR, TRKA; RECEPTORS, NERVE GROWTH FACTOR; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0033539065     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/43705     Document Type: Article

References (30)

1. Snider, W. D. Functions of the neurotrophins during nervous system development: what the knockouts are teaching us. Cell 77, 627-638 (1994).
2. Chao, M. et al. Neurotrophin receptors: mediators of life and death. Brain Res. Rev. 26, 295-301 (1998).
3. Kaplan, D. R., Hempstead, B. L. Martin-Zanca, D., Chao, M. V. & Panda, L. F. The trk proto-oncogene product: a signal transducing receptor for nerve growth factor. Science 252, 554-558 (1991).
4. Urfer, R. et al. An immunoglobulin-like domain determines the specificity of neurotrophin receptors. EMBO J. 14, 2795-2805 (1995).
5. Chao, M. V. Neurotrophin receptors: a window into neuronal differentiation. Neuron 9, 583-593 (1992).
6. Götz, R. et al. Neurotrophin-6 is a new member of the nerve growth factor family. Nature 372, 366-269 (1994).
7. Schneider, R. & Schweiger, M. A novel modular mosaic of cell adhesion motifs in the extracellular domains of the neurogenic trk and trkB tyrosine kinase receptors. Oncogene 6, 1807-1811 (1991).
8. Peréz, P., Coll, P. M., Hempstead, B. L., Martin-Zanca, D. & Chao, M. V. NGF binding to the trk tyrosine kinase receptor requires the extracellular immunoglobulin-like domains. Mol. Cell. Neurosci. 6, 97-105 (1995).
9. Ultsch, M. et al. Crystal structures of the neurotrophin-binding domain of TrkA, TrkB and TrkC. J. Mol. Biol. 290, 149-159 (1999).
10. McDonald, N. Q. et al. New protein fold revealed by a 2.3-Å resolution crystal structure of nerve growth factor. Nature 354, 411-414 (1991).
11. McInnes, C. & Sykes, B. D. Growth factor receptors: structure, mechanism, and drug discovery. Biopolymers 43, 339-366 (1997).
12. Urfer, R. et al. The binding epitopes of neurotrophin-3 to its receptors trkC and gp75 and the design of a multifunctional human neurotrophin. EMBO J. 13, 5896-5909 (1994).
13. Urfer, R. et al. High resolution mapping of the binding site of TrkA for nerve growth factor and TrkC for neurotrophin-3 on the second immunoglobulin-like domain of the Trk receptors. J. Biol. Chem. 273, 5829-5840 (1998).
14. Ibáñez, C. F., Ebendal, T. & Persson, H. Chimeric molecules with multiple neurotrophic activities reveal structural elements determining the specificities of NGF and BDNF. EMBO J. 10, 2105-2110 (1991).
15. Kullander, K. & Ebendal, T. Neurotrophin-3 acquires NGF-like activity after exchange to five NGF amino acid residues: molecular analysis of the sites in NGF mediating the specific interaction with the NGF high affinity receptor. J. Neurosci. Res. 39, 195-210 (1994).
16. Ibáñez, C. F., Ilag, L. L., Murray-Rust, J. & Persson, H. An extended surface of binding to Trk tyrosine kinase receptors in NGF and BDNF allows the engineering of a multifunctional pan-neurotrophin. EMBO J. 12, 2281-2293 (1993).
17. O'Leary, P. D. & Hughes, R. A. Structure-activity relationships of conformationally constrained peptide analogues of loop 2 of brain-derived neurotrophic factor, J. Neurochem. 70, 1712-1721 (1998).
18. Urfer, R., Tsoulfas, P., O'Connell, L. & Presta, L. G. Specificity determinants in neurotrophin-3 and design of nerve growth factor-based trkC agonists by changing central beta-strand bundle residues to their neurotrophin-3 analogs. Biochemistry 36, 4775-4781 (1997).
19. Windisch, J. M., Marksteiner, R. & Schneider, R. Nerve growth factor binding site on TrkA mapped to a single 24-amino acid leucine-rich motif. J. Biol. Chem. 270, 28133-28138 (1995).
20. Windisch, J. M., Marksteiner, R., Lang, M. E., Auer, B. & Schneider, R. Brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 bind to a single leucine-rich motif of TrkB. Biochemistry 34, 11256-11263 (1995).
21. Dechant, G., Tsoulfas, P., Parada, L. F. & Barde, Y. A. The neurotrophin receptor p75 binds neurotrophin-3 on sympathetic neurons with high affinity and specificity. J. Neurosci. 17, 5271-5287 (1997).
22. Ibáñez, C. F. et al. Disruption of the low affinity receptor-binding site in NGF allows neuronal survival and differentiation by binding to the trk gene product. Cell 69, 329-341 (1992).
23. Ryden, M. & Ibanez, C. F. A second determinant of binding to the p75 neurotrophin receptor revealed by alanine-scanning mutagenesis of a conserved loop in nerve growth factor. J. Biol. Chem. 272, 33085-33091 (1997).
24. Wiesmann, C. et al. Crystal structure at 1.7 Å resolution of VEGF in complex with domain 2 of the Flt-1 receptor. Cell 91, 695-704 (1997).
25. Sun, P. D. & Davies, D. R. The cystine-knot growth-factor superfamily. Annu. Rev. Biophys. Biomol. Struct. 24, 269-291 (1995).
26. Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307-326 (1997).
27. CCP4. Programs for protein crystallography. Acta Crystallogr. D 50, 760-763 (1994).
28. Jones, T. A., Zhou, J.-Y., Cowan, S. W. & Kjelgaard, M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A 47, 110-119 (1991).
29. Brünger, A. T., Kuriyan, J. & Karplus, M. Crystallographic R factor refinement by molecular dynamics. Science 235, 458-460 (1987).
30. Laskowski, R. A., MacArthur, M. W., Moss, D. S. & Thornton, J. M. Procheck: a program to check to the stereochemical quality of protein structures. J. Appl. Crystallogr. 26, 283-291 (1993).