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All new compounds were fully characterized. The enantiomeric purity of 4 and 6 was ascertained by cleavage of the reverse prenyl group with TEA and comparison with authentic Cbz-protected amino acid.
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All new compounds were fully characterized. The enantiomeric purity of 4 and 6 was ascertained by cleavage of the reverse prenyl group with TEA and comparison with authentic Cbz-protected amino acid.
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HPLC analysis of the constituent amino acids after hydrolysis of synthetic 1 and derivatization according to Marfey's procedure (Adamson, J. G.; Hong, T.; Crivici, A.; Lajoie, G. Anal. Biochem. 1992, 202, 210) confirmed the presence of L-Ile. Accordingly, macrolactamization proceeded without racemization.
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0013628382
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note
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+): 860.4357; found: 860.4314.
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