A functional assay for centromere-associated sister chromatid cohesion

Science

Volumn 285, Issue 5425, 1999, Pages 254-257

  Megee, Paul C ^a   Koshland, Douglas ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CENTROMERE; DNA DETERMINATION; DNA SEQUENCE; FUNGAL GENETICS; MINICHROMOSOME; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SISTER CHROMATID;

CENTROMERE; CHROMATIDS; CHROMOSOMES, FUNGAL; CONSERVED SEQUENCE; DNA, FUNGAL; G1 PHASE; GREEN FLUORESCENT PROTEINS; IN SITU HYBRIDIZATION, FLUORESCENCE; KINETOCHORES; LUMINESCENT PROTEINS; MITOSIS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE;

FUNGI; MYXOGASTRIA; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 0033538436     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5425.254     Document Type: Article

References (21)

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10. Centromere cassettes contained a 0.3-kb Bam HI CEN3 fragment [D. Koshland et al., Cell 48, 801 (1987)] and a 1.1-kb Hind III fragment encoding URA3, flanked by R recombinase target sites [H. Araki et al., J. Mol. Biol. 225, 25 (1992)]. Centromere cassettes were subcloned into the Bam HI site of pDK222 [D. Koshland, J. C. Kent, L. H. Hartwell, Cell 40, 393 (1985)]. For GFP-tagged minichromosomes, an 11-kb tetracycline operator (tetO) DNA fragment encoding 224 tet repressor-GFP fusion protein (tetR- GFP) binding sites (3) was subdoned into the Xho I site in pDK222-based vectors.
11. Centromere cassettes contained a 0.3-kb Bam HI CEN3 fragment [D. Koshland et al., Cell 48, 801 (1987)] and a 1.1-kb Hind III fragment encoding URA3, flanked by R recombinase target sites [H. Araki et al., J. Mol. Biol. 225, 25 (1992)]. Centromere cassettes were subcloned into the Bam HI site of pDK222 [D. Koshland, J. C. Kent, L. H. Hartwell, Cell 40, 393 (1985)]. For GFP-tagged minichromosomes, an 11-kb tetracycline operator (tetO) DNA fragment encoding 224 tet repressor-GFP fusion protein (tetR-GFP) binding sites (3) was subdoned into the Xho I site in pDK222-based vectors.
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21. Supported in part by NIH grant GM41718. We thank M. K. Raghuraman and R. Ciosk for plasmids and C.-M. Fan, V. Guacci, P. Meluh, and members of the Koshland Laboratory for valuable comments on the manuscript. P.C.M. was the recipient of a Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation Fellowship DRG-1335.