-
5
-
-
0029845117
-
-
T. Takahashi, I. D. Forsythe, T. Tsujimoto, M. Barnes-Davies, K. Onodera, Science 274, 594 (1996).
-
(1996)
Science
, vol.274
, pp. 594
-
-
Takahashi, T.1
Forsythe, I.D.2
Tsujimoto, T.3
Barnes-Davies, M.4
Onodera, K.5
-
6
-
-
0029921401
-
-
S. R. Ikeda, Nature 380, 255 (1996); 5. Herlitze et al., ibid., p. 258.
-
(1996)
Nature
, vol.380
, pp. 255
-
-
Ikeda, S.R.1
-
7
-
-
0029921401
-
-
S. R. Ikeda, Nature 380, 255 (1996); S. Herlitze et al., ibid., p. 258.
-
Nature
, pp. 258
-
-
Herlitze, S.1
-
8
-
-
0029973079
-
-
R. Shigemoto et al., ibid. 381, 523 (1996); R. Shigemoto et al., J. Neurosci. 17, 7503 (1997).
-
(1996)
Nature
, vol.381
, pp. 523
-
-
Shigemoto, R.1
-
9
-
-
0030821474
-
-
R. Shigemoto et al., ibid. 381, 523 (1996); R. Shigemoto et al., J. Neurosci. 17, 7503 (1997).
-
(1997)
J. Neurosci.
, vol.17
, pp. 7503
-
-
Shigemoto, R.1
-
10
-
-
0031035665
-
-
J. A. Saugstad, J. M. Kinzie, M. M. Shinohara, T. P. Sergerson, G. L. Westbrook, Mol. Pharmacol. 51, 119 (1997).
-
(1997)
Mol. Pharmacol.
, vol.51
, pp. 119
-
-
Saugstad, J.A.1
Kinzie, J.M.2
Shinohara, M.M.3
Sergerson, T.P.4
Westbrook, G.L.5
-
11
-
-
0027955170
-
-
To generate sequences encoding the COOH-terminal region of mGluR 7 [N. Okamoto et al., J. Biol. Chem. 269, 1231 (1994)], we used oligonucleotides complementary to the codons of amino acids 851 to 857 (HPELNVQ; gagattccaccctgaactcaatgtccag) and 910 to 916 (YNNLVIstop; ctgtcgacttagataaccaggttattata) in a standard PCR on mouse brain cDNA at annealing temperatures of 52°C (26). This generated two products corresponding to cDNAs encoding the known COOH-terminal region of mGluR 7 (mGluR 7A) and a recently reported splice variant [P. J. Flor et al., Neuropharmacology 36, 153 (1997)] containing an additional 81-base pair insertion (mGluR 7B). Full-length mGluR 7A-ΔCaM and truncated mGluR 7A tail sequences were also generated by PCR using a similar procedure. All amplification products were verified by automated DNA sequencing.
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 1231
-
-
Okamoto, N.1
-
12
-
-
0030996238
-
-
To generate sequences encoding the COOH-terminal region of mGluR 7 [N. Okamoto et al., J. Biol. Chem. 269, 1231 (1994)], we used oligonucleotides complementary to the codons of amino acids 851 to 857 (HPELNVQ; gagattccaccctgaactcaatgtccag) and 910 to 916 (YNNLVIstop; ctgtcgacttagataaccaggttattata) in a standard PCR on mouse brain cDNA at annealing temperatures of 52°C (26). This generated two products corresponding to cDNAs encoding the known COOH-terminal region of mGluR 7 (mGluR 7A) and a recently reported splice variant [P. J. Flor et al., Neuropharmacology 36, 153 (1997)] containing an additional 81-base pair insertion (mGluR 7B). Full-length mGluR 7A-ΔCaM and truncated mGluR 7A tail sequences were also generated by PCR using a similar procedure. All amplification products were verified by automated DNA sequencing.
-
(1997)
Neuropharmacology
, vol.36
, pp. 153
-
-
Flor, P.J.1
-
16
-
-
0345332319
-
-
Affinity measurements were done by fluorescence spectroscopy using dansylated calmodulin
-
Affinity measurements were done by fluorescence spectroscopy using dansylated calmodulin.
-
-
-
-
19
-
-
0021360003
-
-
P. C. Leung, W. A. Taylor, J. H. Wang, C. L. Tipton, J. Biol. Chem. 259, 2742 (1984).
-
(1984)
J. Biol. Chem.
, vol.259
, pp. 2742
-
-
Leung, P.C.1
Taylor, W.A.2
Wang, J.H.3
Tipton, C.L.4
-
20
-
-
0345332316
-
-
unpublished data
-
V. O'Connor, unpublished data.
-
-
-
O'Connor, V.1
-
21
-
-
0028600610
-
-
2+ did not affect the binding of Gβγ to GST-7A. After repeated washing, bound proteins were eluted with 30 mM glutathione or SDS sample buffer. Immunoblots were done with antiserum 7 (Gβ-common) or antiserum 11 (Gα-common) (29).
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 32077
-
-
Jockers, R.1
-
22
-
-
0028142193
-
-
2+ did not affect the binding of Gβγ to GST-7A. After repeated washing, bound proteins were eluted with 30 mM glutathione or SDS sample buffer. Immunoblots were done with antiserum 7 (Gβ-common) or antiserum 11 (Gα-common) (29).
-
(1994)
Methods Enzymol.
, vol.327
, pp. 254
-
-
Mumby, S.M.1
Linder, M.E.2
-
23
-
-
0345332315
-
-
2+ did not affect the binding of Gβγ to GST-7A. After repeated washing, bound proteins were eluted with 30 mM glutathione or SDS sample buffer. Immunoblots were done with antiserum 7 (Gβ-common) or antiserum 11 (Gα-common) (29)
-
2+ did not affect the binding of Gβγ to GST-7A. After repeated washing, bound proteins were eluted with 30 mM glutathione or SDS sample buffer. Immunoblots were done with antiserum 7 (Gβ-common) or antiserum 11 (Gα-common) (29).
-
-
-
-
26
-
-
0025788797
-
-
2, 20 mM glucose, and 10 mM Hepes, adjusted to pH 7.4 with NaOH. Drug application was performed as described (20). Postsynaptic glutamate responses were elicited by the direct application of 100 μM glutamate
-
2, 20 mM glucose, and 10 mM Hepes, adjusted to pH 7.4 with NaOH. Drug application was performed as described (20). Postsynaptic glutamate responses were elicited by the direct application of 100 μM glutamate.
-
(1991)
Proc. Natl. Acad. Sci. U.S.A.
, vol.88
, pp. 7834
-
-
Bekkers, J.M.1
Stevens, C.F.2
-
28
-
-
0344901257
-
-
data not shown
-
S. Boehm, data not shown.
-
-
-
Boehm, S.1
-
29
-
-
0029863890
-
-
S. R. Bradley, A. I. Levey, S. M. Hersch, P. J. Conn, J. Neurosci. 16, 2044 (1996).
-
(1996)
J. Neurosci.
, vol.16
, pp. 2044
-
-
Bradley, S.R.1
Levey, A.I.2
Hersch, S.M.3
Conn, P.J.4
-
30
-
-
0030174952
-
-
N. K. Thomas, D. I. Jane, H. W. Tse, J. C. Watkins, Neuropharmacology 35, 637 (1996).
-
(1996)
Neuropharmacology
, vol.35
, pp. 637
-
-
Thomas, N.K.1
Jane, D.I.2
Tse, H.W.3
Watkins, J.C.4
-
33
-
-
0344038419
-
-
Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
-
-
-
Ala, A.1
Cys, C.2
Asp, D.3
Glu, E.4
Phe, F.5
Gly, G.6
His, H.7
Ile, I.8
Lys, K.9
Leu, L.10
Met, M.11
Asn, N.12
Pro, P.13
Gln, Q.14
Arg, R.15
Ser, S.16
Thr, T.17
Val, V.18
Trp, W.19
Tyr, Y.20
more..
-
35
-
-
0345332307
-
-
unpublished data
-
O. El Far, unpublished data.
-
-
-
El Far, O.1
-
36
-
-
0028972629
-
-
C. Nanoff, T. Mitterauer, F. Roka, M. Hohenegger, M. Freissmuth, Mol. Pharmacol. 48, 806 (1995).
-
(1995)
M. Freissmuth, Mol. Pharmacol.
, vol.48
, pp. 806
-
-
Nanoff, C.1
Mitterauer, T.2
Roka, F.3
Hohenegger, M.4
-
37
-
-
0028987847
-
-
P. F. Chapman, B. G. Frenguelli, A. Smith, C. M. Chen, A. J. Silva, Neuron 14, 591 (1995).
-
(1995)
Neuron
, vol.14
, pp. 591
-
-
Chapman, P.F.1
Frenguelli, B.G.2
Smith, A.3
Chen, C.M.4
Silva, A.J.5
-
38
-
-
0032189766
-
-
X. M. Xia et al., Nature 395, 503 (1998).
-
(1998)
Nature
, vol.395
, pp. 503
-
-
Xia, X.M.1
-
39
-
-
0033551361
-
-
A. Lee et al., Nature 399, 155 (1999).
-
(1999)
Nature
, vol.399
, pp. 155
-
-
Lee, A.1
-
40
-
-
0031036195
-
-
M. Scanziani, P. A. Salin, K. E. Vogt, R. C. Malenka, R. A. Nicoll, Nature 385, 630 (1997).
-
(1997)
Nature
, vol.385
, pp. 630
-
-
Scanziani, M.1
Salin, P.A.2
Vogt, K.E.3
Malenka, R.C.4
Nicoll, R.A.5
-
41
-
-
0344038417
-
-
Supported by Deutsche Forschungsgemeinschaft (H.B.), Fonds der Chemischen Industrie (H.B.), the Human Frontier Science Program Organization (HFSPO; H.B.), and the Austrian Science Foundation (S.B., M.F., and C.N.). O.E.F. was supported by a HFSPO postdoctoral fellowship, and E.B.C. by a grant from the European Community
-
We thank R. Shigemoto for mGluR 7A antisera, S. Nakanishi for cDNA, A. Niehuis, A. Motejlek, and E. Wischmeyer for experimental assistance, and M. Baier and M. Reitz for secretarial help. Supported by Deutsche Forschungsgemeinschaft (H.B.), Fonds der Chemischen Industrie (H.B.), the Human Frontier Science Program Organization (HFSPO; H.B.), and the Austrian Science Foundation (S.B., M.F., and C.N.). O.E.F. was supported by a HFSPO postdoctoral fellowship, and E.B.C. by a grant from the European Community.
-
-
-
Shigemoto, R.1
Nakanishi, S.2
Niehuis, A.3
Motejlek, A.4
Wischmeyer, E.5
Baier, M.6
Reitz, M.7
|