Oxysterol-induced cell death in U937 and HepG2 cells at reduced and normal serum concentrations

European Journal of Nutrition

Volumn 38, Issue 6, 1999, Pages 255-262

  O'Callaghan, Y C ^a   Woods, J A ^a   O'Brien, N M ^a  

^a UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

Apoptosis; Cell death; HepG2 cells; Oxysterol; U937 cells

Indexed keywords

25 HYDROXYCHOLESTEROL; 7BETA HYDROXYCHOLESTEROL; CHOLESTEROL; DNA FRAGMENT; ETHIDIUM BROMIDE; FLUORESCEIN DIACETATE; HOE 33342; OXYSTEROL;

APOPTOSIS; ARTICLE; CELL DEATH; CELL PROLIFERATION; CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; HUMAN; HUMAN CELL;

APOPTOSIS; CELL COUNT; CELL DIVISION; CELL SURVIVAL; CULTURE MEDIA; DNA FRAGMENTATION; ELECTROPHORESIS, AGAR GEL; FETAL BLOOD; FLOW CYTOMETRY; HUMANS; HYDROXYCHOLESTEROLS; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; TUMOR CELLS, CULTURED; U937 CELLS;

ANIMALIA;

EID: 0033504017     PISSN: 14366207     EISSN: None     Source Type: Journal    
DOI: 10.1007/s003940050075     Document Type: Article

References (27)

1. Smith LL, Johnson BH (1989) Biological activities of oxysterols. Free Radical Bio Med 7:285-332
2. Guardiola F, Codony R, Addis PB, Rafecas M, Boatella J (1996) Biological effects of oxysterols: current status. Food Chem Toxicol 34:193-211
3. Paniangvait P, King AJ, Jones AD, German BG (1995) Cholesterol oxides in foods of animal origin. J Food Sci 60:1159-1174
4. Lizard G, Deckert V, Dubrez L, Moisant M, Gambert P, Lagrost L (1996) Induction of apoptosis in endothelial cells treated with cholesterol oxides. Am J Pathol 148:1625-1638
5. Tsai LS, Hudson CA (1985) Cholesterol oxides in commercial dry egg products: quantification. J Food Sci 50:229-237
6. Zhang WB, Addis PB, Krick TP (1991) Quantification of 5α-cholestane-3β,5,6β-triol and other cholesterol oxidation products in fast food french fried potatoes. J Food Sci 56:716-718
7. Saucier SE, Kandutsch AA, Gayen AK, Swahn DK, Spencer TA (1989) Oxysterol regulators of 3-hydroxy-3-methylglutaryl-CoA reductase in liver. J Biol Chem 264:6863-6869
8. Christ M, Luu B, Mejia JE, Moosbrugger I, Bischoff P (1993) Apoptosis induced by oxysterols in murine lymphoma cells and in normal thymocytes. Immunology 78:455-460
9. Linseisen J, Wolfram G (1998) Origin, metabolism and adverse health effects of cholesterol oxidation products. Fett/Lipid 100:211-218
10. Smith LL, Smart VB, Made Gowda NM (1986) Mutagenic sterol hydroperoxides. Mutat Res 161:39-48
11. Woods JA, O'Brien NM (1998) Inveŝ tigation of the potential genotoxicity of cholesterol oxidation products in two mammalian fibroblast cell lines. Nutr Cancer 31:192-198
12. Hwang PL (1991) Biological activities of oxygenated sterols: physiological and pathological implications. Bioeŝ says 13:583-589
13. Thompson CB (1995) Apoptosis in the pathogenesis and treatment of disease. Science 267:1456-1462
14. Aupeix K, Weltin D, Mejia JE, Christ M, Marchai J, Freyssinet J, Bischoff P (1995) Oxysterol induced apoptosis in human monocytic cell lines. Immunobiology 194:415-428
15. Lizard G, Gueldry S, Sordet O, Monier S, Athias A, Miguet C, Bessede G, Lemaire S, Solary E, Gambert P (1998) Glutathione is implied in the control of 7-ketocholesterol-induced apoptosis, which is associated with radical oxygen species production. FASEB J. 12: 1651-1663
16. Hietter H, Trifilieff E, Richert L, Beck J, Luu B, Ourisson G (1984) Antagonistic action of cholesterol towards the toxicity of hydroxysterols on cultured hepatoma cells. Biochem Biophys Res Comm 120:657-664
17. Mowles JM (1990) Mycoplasma Detection. In: Pollard JW, Walker JM (eds) Methods in Molecular Biology Vol. V: Animal Cell Culture, Humana Press, Clifton, NJ, pp 65-74
18. Strauss GHS (1991) Non-random cell killing in cryopreservation: implications for performance of the battery of leukocyte tests (BLT). I. Toxic and immunotoxic effects. Mutat Res 252:1-15
19. Dubrez L, Savoy I, Hamman A, Solary E (1996) Pivotal role of a DEVD-sensitive step in etoposide-induced and Fas-mediated apoptotic pathways. EMBO J 15:5504-5512
20. Swat W, Ignatowicz L, Kisielow P (1991) Detection of apoptosis of immature CD4+8+ thymocytes by flow cytometry. J Immunol Methods 137: 79-87
21. Nordmann P, Diez-Ibanez M, Chessebeuf-Padieu M, Luu B, Mack G, Mersels M (1989) Toxic effects of 7β-hydroxycholesterol on rat liver primary cultures, epithelial lines and cocultures. Cell Biol Toxicol 5:261-270
22. Bakos JT, Johnson BH, Thompson EB (1993) Oxysterol-induced cell death in human leukemic T-cells correlates with oxysterol binding protein occupancy and is independent of glucocorticoid-induced apoptosis. J Steroid Biochem 46:415-426
23. Ayala-Torres S, Johnson BH, Thompson BE (1994) Oxysterol sensitive and resistant lymphoid cells: correlation with regulation of cellular nucleic acid binding protein mRNA. J Steroid Biochem 48:307-315
24. Lund EG, Kerr TA, Sakai J, Li W-P, Russell DW (1998) cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesise a potent oxysterol regulator of lipid metabolism. J Biol Chem 273:34316-34327
25. Janowski BA, Willy PJ, Devi TR, Falck JR Mangelsdorf DJ (1996) An oxysterol signalling pathway mediated by the nuclear receptor LXR alpha. Nature 383:728-731
26. Harada K, Ishibashi S, Miyashita T, Osuga J, Yagyu H, Ohashi K, Yazaki Y, Yamada N (1997) Bcl-2 protein inhibits oxysterol-induced apoptosis through suppressing CPP32-mediated pathway. FEBS Letters 411:63-66
27. Lizard G, Lemarie S, Monier S, Gueldry S, Néel D, Gambert P (1997) Induction of apoptosis and of interleukin-1β secretion by 7β-hydroxycholesterol and 7-ketocholesterol: Partial inhibition by Bcl-2 overexpression. FEBS Letters 419:276-280