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1
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0029444559
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Surrogate cells or Trojan horses
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The discovery of liposomes •The author who is the founder of the liposome research field gives an autobiographical history of liposome science
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Bangham AD. Surrogate cells or Trojan horses. The discovery of liposomes. Bioessays 1995;17:1081-1088. •The author who is the founder of the liposome research field gives an autobiographical history of liposome science.
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(1995)
Bioessays
, vol.17
, pp. 1081-1088
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Bangham, A.D.1
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2
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0001029147
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Preparation of giant vesicles by external AC electric fields: Kinetics and applications
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•A homogeneous population of perfectly spherical and unilamellar GLs are formed reproducibly by the swelling of phospholipids under the influence of an AC electric field. This broadly applicable method overcomes problems of non-reproducibility and low yield in the preparation of GLs
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Angelova MI, Soleau S, Meleard P, Faucon J, Bothorel P: Preparation of giant vesicles by external AC electric fields: kinetics and applications. Prog Colloid Polym Sci 1992;89:127-131. •A homogeneous population of perfectly spherical and unilamellar GLs are formed reproducibly by the swelling of phospholipids under the influence of an AC electric field. This broadly applicable method overcomes problems of non-reproducibility and low yield in the preparation of GLs.
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(1992)
Prog Colloid Polym Sci
, vol.89
, pp. 127-131
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Angelova, M.I.1
Soleau, S.2
Meleard, P.3
Faucon, J.4
Bothorel, P.5
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3
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0030051845
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Shape change and physical properties of giant phospholipid vesicles prepared in the presence of an AC electric field
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Mathivet L., Cribier S., Devaux P.F. Shape change and physical properties of giant phospholipid vesicles prepared in the presence of an AC electric field. Biophys J. 70:1996;1112-1121.
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Biophys J
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Mathivet, L.1
Cribier, S.2
Devaux, P.F.3
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4
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0029819082
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Preparation of giant myelin vesicles and proteoliposomes to register ionic channels
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Regueiro P., Monreal J., Diaz R.S., Sierra F. Preparation of giant myelin vesicles and proteoliposomes to register ionic channels. J Neurochem. 67:1996;2146-2154.
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(1996)
J Neurochem
, vol.67
, pp. 2146-2154
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Regueiro, P.1
Monreal, J.2
Diaz, R.S.3
Sierra, F.4
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5
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0029752766
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Preparation of giant liposomes in physiological conditions and their characterization under an optical microscope
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••A new method for reproducible preparation of GLs under physiological ionic conditions is developed by introducing prehydration and is investigated for lipid compositions. The unilamellarity of GLs is clearly shown by the quantitative measurements of images in phase-contrast and fluorescence microscopy as well as by the determination of elastic modulus
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Akashi K, Miyata H, Itoh H, Kinosita K Jr. Preparation of giant liposomes in physiological conditions and their characterization under an optical microscope. Biophys J 1996;71:3242-3250. ••A new method for reproducible preparation of GLs under physiological ionic conditions is developed by introducing prehydration and is investigated for lipid compositions. The unilamellarity of GLs is clearly shown by the quantitative measurements of images in phase-contrast and fluorescence microscopy as well as by the determination of elastic modulus.
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(1996)
Biophys J
, vol.71
, pp. 3242-3250
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Akashi, K.1
Miyata, H.2
Itoh, H.3
Kinosita K., Jr.4
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6
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0031806924
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Formation of giant liposomes promoted by divalent cations: Critical role of electrostatic repulsion
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2+ are demonstrated to promote the formation of GLs
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2+ are demonstrated to promote the formation of GLs.
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(1998)
Biophys J
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Akashi, K.1
Miyata, H.2
Itoh, H.3
Kinosita K., Jr.4
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7
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0029957224
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Rapid preparation of giant unilamellar vesicles
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•A rapid evaporation method is developed that produces unilamellar GLs within only 2 min in various buffers. This method is anticipated to be broadly applicable for studying labile biomolecules in GLs
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Moscho A, Orwar O, Chiu DT, Modi BP, Zare RN. Rapid preparation of giant unilamellar vesicles. Proc Natl Acad Sci USA 1996;93:11443-11447. •A rapid evaporation method is developed that produces unilamellar GLs within only 2 min in various buffers. This method is anticipated to be broadly applicable for studying labile biomolecules in GLs.
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(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 11443-11447
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Moscho, A.1
Orwar, O.2
Chiu, D.T.3
Modi, B.P.4
Zare, R.N.5
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8
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0021755796
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Transformation pathways of liposomes
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••This work is the first systematic demonstration of GL transformations. GLs are found to transform sequentially in a well-defined manner through one of several transformation pathways. The driving force for the transformations is found to be osmotic pressure that decrease the liposome volume, thereby altering the surface-to-volume ratio
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Hotani H. Transformation pathways of liposomes. J Mol Biol 1984;178:113-120. ••This work is the first systematic demonstration of GL transformations. GLs are found to transform sequentially in a well-defined manner through one of several transformation pathways. The driving force for the transformations is found to be osmotic pressure that decrease the liposome volume, thereby altering the surface-to-volume ratio.
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(1984)
J Mol Biol
, vol.178
, pp. 113-120
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Hotani, H.1
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9
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0025937302
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Shape transitions and shape stability of giant phospholipid vesicles in pure water induced by area-to-volume changes
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Kas J., Sackmann E. Shape transitions and shape stability of giant phospholipid vesicles in pure water induced by area-to-volume changes. Biophys J. 60:1991;825-844.
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Biophys J
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Kas, J.1
Sackmann, E.2
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10
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0027517911
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Budding and fission of vesicles
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Dobereiner H.G., Kas J., Noppl D., Sprenger I., Sackmann E. Budding and fission of vesicles. Biophys J. 65:1993;1396-1403.
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(1993)
Biophys J
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Dobereiner, H.G.1
Kas, J.2
Noppl, D.3
Sprenger, I.4
Sackmann, E.5
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11
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0026651919
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Shape changes of giant liposomes induced by an asymmetric transmembrane distribution of phospholipids
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•Asymmetry of lipid composition between the outer and inner leaflets in lipid bilayer membranes is shown to be the important factor in determining liposome shape. The alteration of phospholipid transmembrane distribution causes the shape change of GLs
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Farge E, Devaux PF. Shape changes of giant liposomes induced by an asymmetric transmembrane distribution of phospholipids. Biophys J 1992;61:347-357. •Asymmetry of lipid composition between the outer and inner leaflets in lipid bilayer membranes is shown to be the important factor in determining liposome shape. The alteration of phospholipid transmembrane distribution causes the shape change of GLs.
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(1992)
Biophys J
, vol.61
, pp. 347-357
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Farge, E.1
Devaux, P.F.2
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12
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0029122152
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Influence of transbilayer area asymmetry on the morphology of large unilamellar vesicles
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Mui B.L., Dobereiner H.G., Madden T.D., Cullis P.R. Influence of transbilayer area asymmetry on the morphology of large unilamellar vesicles. Biophys J. 69:1995;930-941.
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Biophys J
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, pp. 930-941
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Mui, B.L.1
Dobereiner, H.G.2
Madden, T.D.3
Cullis, P.R.4
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14
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0031837977
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Dynamic excitations in membranes induced by optical tweezers
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••The authors give a thorough phenomenology in transformation of GLs caused by irradiation with a laser tweezers. A variety of dynamic instabilities and shape transformations are described, including pearling instability, expulsion of vesicles, and the formation of tubules. Their physical picture of the laser-membrane interaction is based on the generation of tension in the bilayer and the loss of surface area
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Bar-Ziv R, Moses E, Nelson P. Dynamic excitations in membranes induced by optical tweezers. Biophys J 1998;75:294-320. ••The authors give a thorough phenomenology in transformation of GLs caused by irradiation with a laser tweezers. A variety of dynamic instabilities and shape transformations are described, including pearling instability, expulsion of vesicles, and the formation of tubules. Their physical picture of the laser-membrane interaction is based on the generation of tension in the bilayer and the loss of surface area.
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(1998)
Biophys J
, vol.75
, pp. 294-320
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Bar-Ziv, R.1
Moses, E.2
Nelson, P.3
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15
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0027591979
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The mechanics of axially symmetric liposomes
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•Based on the finite-deformation mechanical theory of axisymmetric thin shells, the authors analyze video images of GL transformations when their interior volume decreases steadily
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Pamplona DC, Calladine CR. The mechanics of axially symmetric liposomes. J Biomech Eng 1993;115:149-159. •Based on the finite-deformation mechanical theory of axisymmetric thin shells, the authors analyze video images of GL transformations when their interior volume decreases steadily.
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(1993)
J Biomech Eng
, vol.115
, pp. 149-159
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Pamplona, D.C.1
Calladine, C.R.2
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Aspects of the mechanics of lobed liposomes
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Pamplona D.C., Calladine C.R. Aspects of the mechanics of lobed liposomes. J Biomech Eng. 118:1996;482-488.
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J Biomech Eng
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Pamplona, D.C.1
Calladine, C.R.2
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17
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0027408212
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Tension-stabilized pores in giant vesicles: Determination of pore size and pore line tension
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•GLs held on a micropipette are subjected to electric pulses, resulting in the formation of pores in the liposome membrane. By adjusting the membrane tension, the pore lifetime can be varied from tenths of a second to several seconds
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Zhelev DV, Needham D. Tension-stabilized pores in giant vesicles: determination of pore size and pore line tension. Biochim Biophys Acta 1993;1147:89-104. •GLs held on a micropipette are subjected to electric pulses, resulting in the formation of pores in the liposome membrane. By adjusting the membrane tension, the pore lifetime can be varied from tenths of a second to several seconds.
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(1993)
Biochim Biophys Acta
, vol.1147
, pp. 89-104
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Zhelev, D.V.1
Needham, D.2
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0030972503
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Dynamically stabilized pores in bilayer membranes
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Moroz J.D., Nelson P. Dynamically stabilized pores in bilayer membranes. Biophys J. 72:1997;2211-2216.
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Biophys J
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Moroz, J.D.1
Nelson, P.2
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19
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0032477865
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Opening-up of liposomal membranes by talin
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••A cytoskeletal submembranous protein, talin, is found to make stable holes in GLs. A liposome opens a big hole, subsequently transforms into a cup-shaped one and finally into a lipid bilayer sheet. These shape changes are reversed by protein dilution. Talin is localized mainly along the membrane verges. This finding refutes the established dogma that all lipid bilayer membranes inevitably form closed vesicles and suggests that talin is a useful tool for manipulating liposome topology
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Saitoh A, Takiguchi K, Tanaka Y, Hotani H. Opening-up of liposomal membranes by talin. Proc Natl Acad Sci USA 1998;95:1026-1031. ••A cytoskeletal submembranous protein, talin, is found to make stable holes in GLs. A liposome opens a big hole, subsequently transforms into a cup-shaped one and finally into a lipid bilayer sheet. These shape changes are reversed by protein dilution. Talin is localized mainly along the membrane verges. This finding refutes the established dogma that all lipid bilayer membranes inevitably form closed vesicles and suggests that talin is a useful tool for manipulating liposome topology.
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(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 1026-1031
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Saitoh, A.1
Takiguchi, K.2
Tanaka, Y.3
Hotani, H.4
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20
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0025204589
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Dynamic features of microtubules as visualized by dark-field microscopy
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•This is the first demonstration of liposome transformation caused by cytoskeleton growth. A spherical liposome transforms into a rugby-ball shape due to the mechanical force generated by MT polymerization, and finally into a characteristic shape consisting of a central ellipsoid and two straight tubes. Living cell-like shaped GLs are also developed
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Hotani H, Miyamoto H. Dynamic features of microtubules as visualized by dark-field microscopy. Adv Biophys 1990;26:135-156. •This is the first demonstration of liposome transformation caused by cytoskeleton growth. A spherical liposome transforms into a rugby-ball shape due to the mechanical force generated by MT polymerization, and finally into a characteristic shape consisting of a central ellipsoid and two straight tubes. Living cell-like shaped GLs are also developed.
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(1990)
Adv Biophys
, vol.26
, pp. 135-156
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Hotani, H.1
Miyamoto, H.2
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21
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0031372605
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Mechanics of microtubule-based membrane extension
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Fygenson D.K., Marko J.F., Libchaber A. Mechanics of microtubule-based membrane extension. Phys Rev Lett. 79:1997;4497-4500.
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Phys Rev Lett
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Fygenson, D.K.1
Marko, J.F.2
Libchaber, A.3
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0000579527
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Vesicle deformation by microtubules: A phase diagram
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Emsellem V., Cardoso O., Tabeling P. Vesicle deformation by microtubules: a phase diagram. Phys Rev E. 58:1998;4807-4810.
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Emsellem, V.1
Cardoso, O.2
Tabeling, P.3
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23
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0032545175
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Morphological transformation of liposomes caused by assembly of encapsulated tubulin and determination of shape by microtubule-associated proteins (MAPs)
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•Bipolar GLs are found to transform into monopolar shapes by fluctuation of the central sphere toward one of the ends without shortening of the tube portion. MAPs mediate binding between MTs and the liposomal membrane, and thereby prevent this transformation
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Kaneko T, Itoh TJ, Hotani H. Morphological transformation of liposomes caused by assembly of encapsulated tubulin and determination of shape by microtubule-associated proteins (MAPs). J Mol Biol 1998;284:1671-1681. •Bipolar GLs are found to transform into monopolar shapes by fluctuation of the central sphere toward one of the ends without shortening of the tube portion. MAPs mediate binding between MTs and the liposomal membrane, and thereby prevent this transformation.
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(1998)
J Mol Biol
, vol.284
, pp. 1671-1681
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Kaneko, T.1
Itoh, T.J.2
Hotani, H.3
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24
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0000256477
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Theoretical analysis of the formation of membrane microtubes on axially strained vesicles
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••The authors theoretically analyze the formation of membrane tubes caused by a force applied at the liposome poles. Equilibrium shapes are obtained by seeking the minimum of local and non-local bending energies of the membrane
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Bozic B, Sventina S, Zeks B. Theoretical analysis of the formation of membrane microtubes on axially strained vesicles. Phys Rev E 1997;55:5834-5842. ••The authors theoretically analyze the formation of membrane tubes caused by a force applied at the liposome poles. Equilibrium shapes are obtained by seeking the minimum of local and non-local bending energies of the membrane.
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Phys Rev e
, vol.55
, pp. 5834-5842
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Bozic, B.1
Sventina, S.2
Zeks, B.3
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25
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0344348825
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Vesicle deformation by an axial load: From elongated shapes to tethered vesicles
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•The transformation phenomenon of a GL from spherical to bipolar shape is analyzed by general mathematical methods to obtain shapes, including their stabilities. The authors investigate the force-extension relation of a liposome, and demonstrate how the stretched liposome transforms into a bipolar shape. Beautiful microscopic images of this transformation are shown using micropipettes
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Heinrich V, Bozic B, Svetina S, Zeks B. Vesicle deformation by an axial load: from elongated shapes to tethered vesicles. Biophys J 1999;76:2056-2071. •The transformation phenomenon of a GL from spherical to bipolar shape is analyzed by general mathematical methods to obtain shapes, including their stabilities. The authors investigate the force-extension relation of a liposome, and demonstrate how the stretched liposome transforms into a bipolar shape. Beautiful microscopic images of this transformation are shown using micropipettes.
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(1999)
Biophys J
, vol.76
, pp. 2056-2071
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Heinrich, V.1
Bozic, B.2
Svetina, S.3
Zeks, B.4
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26
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0032373447
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Theoretical analysis of shape transformations of liposomes caused by microtubule assembly
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••Using a mathematical model based on the notion of the minimum bending energy of the liposome membrane, the authors investigate mechanical aspects of the shape transformation of liposomes caused by MT growth. Numerical analysis gives a series of shapes which are similar to those observed in experiments. To obtain the bipolar shape, the inside positive pressure is found to be indispensable in this theoretical model
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Umeda T, Nakajima H, Hotani H. Theoretical analysis of shape transformations of liposomes caused by microtubule assembly. J Phys Soc Jpn 1998;67:682-688. ••Using a mathematical model based on the notion of the minimum bending energy of the liposome membrane, the authors investigate mechanical aspects of the shape transformation of liposomes caused by MT growth. Numerical analysis gives a series of shapes which are similar to those observed in experiments. To obtain the bipolar shape, the inside positive pressure is found to be indispensable in this theoretical model.
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J Phys Soc Jpn
, vol.67
, pp. 682-688
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Umeda, T.1
Nakajima, H.2
Hotani, H.3
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27
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0026482154
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Morphological changes in liposomes caused by polymerization of encapsulated actin and spontaneous formation of actin bundles
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•Polymerization of the encapsulated G-actin into F-actin in GLs causes the subsequent shape changes of spherical GL into dumbbell or disk shape. Polarization microscopy shows that F-actins spontaneously form thick bundles in the GLs and that the bundles lay contiguous to the periphery of the liposome
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Miyata H, Hotani H. Morphological changes in liposomes caused by polymerization of encapsulated actin and spontaneous formation of actin bundles. Proc Natl Acad Sci USA 1992;89:11547-11551. •Polymerization of the encapsulated G-actin into F-actin in GLs causes the subsequent shape changes of spherical GL into dumbbell or disk shape. Polarization microscopy shows that F-actins spontaneously form thick bundles in the GLs and that the bundles lay contiguous to the periphery of the liposome.
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(1992)
Proc Natl Acad Sci USA
, vol.89
, pp. 11547-11551
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Miyata, H.1
Hotani, H.2
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0028136029
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Transformation of actin-encapsulating liposomes induced by cytochalasin D
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Miyata H., Kinosita K. Jr. Transformation of actin-encapsulating liposomes induced by cytochalasin D. Biophys J. 67:1994;922-928.
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Biophys J
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Miyata, H.1
Kinosita K., Jr.2
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Shape changes of self-assembled actin bilayer composite membranes
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Hackl W., Barmann M., Sackmann E. Shape changes of self-assembled actin bilayer composite membranes. Phys Rev L. 80:1998;1786-1789.
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Morphogenesis of liposomes encapsulating actin depends on the type of actin-crosslinking
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•The encapsulated F-actins form bundles or gels, depending on the type of actin-crosslinking protein that is co-encapsulated, causing various transformations of GLs. This work reveals that the manner of crosslinking (i.e. the distance and angular flexibility between adjacent crosslinked F-actins) is essential for morphogenesis
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Honda M, Takiguchi K, Ishikawa S, Hotani H. Morphogenesis of liposomes encapsulating actin depends on the type of actin-crosslinking. J Mol Biol 1999;287:293-300. •The encapsulated F-actins form bundles or gels, depending on the type of actin-crosslinking protein that is co-encapsulated, causing various transformations of GLs. This work reveals that the manner of crosslinking (i.e. the distance and angular flexibility between adjacent crosslinked F-actins) is essential for morphogenesis.
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J Mol Biol
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Honda, M.1
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Micropipette manipulation technique for the monitoring of pH-dependent membrane lysis as induced by the fusion peptide of influenza virus
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Soltesz S.A., Hammer D.A. Micropipette manipulation technique for the monitoring of pH-dependent membrane lysis as induced by the fusion peptide of influenza virus. Biophys J. 68:1995;315-325.
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Soltesz S.A., Hammer D.A. Lysis of large unilamellar vesicles induced by analogs of the fusion peptide of influenza virus hemagglutinin. J Colloid Interface Sci. 186:1997;399-409.
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Outer leaflet-packing defects promote poly(ethylene glycol)-mediated fusion of large unilamellar vesicles
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The mechanism of detergent solubilization of liposomes and protein-containing membranes
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•This work suggests that detergent solubilization proceeds by a combination of (1) a transbilayer attack followed by a flip-flop of detergent molecules, and (2) extraction of membrane components directly by detergent micelles
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Kragh-Hansen U, le Maire M, Moller JV. The mechanism of detergent solubilization of liposomes and protein-containing membranes. Biophys J 1998;75:2932-2946. •This work suggests that detergent solubilization proceeds by a combination of (1) a transbilayer attack followed by a flip-flop of detergent molecules, and (2) extraction of membrane components directly by detergent micelles.
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Giant liposomes: A model system in which to obtain patch-clamp recordings of ionic channels
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•This early work shows that GLs containing acetylcholine receptors can be used to monitor the activity of ionic channels using patch-clamp techniques
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Riquelme G, Lopez E, Garcia-Segura LM, Ferragut JA, Gonzalez-Ros JM. Giant liposomes: a model system in which to obtain patch-clamp recordings of ionic channels. Biochemistry 1990;29:11215-11222. •This early work shows that GLs containing acetylcholine receptors can be used to monitor the activity of ionic channels using patch-clamp techniques.
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•This work demonstrates the possibility of using GLs for direct visualization of the progress of a chemical reaction. Microinjection of snake venom phospholipase A2 inside a GL results in a slow and constant decrease in its size, until it eventually disappears
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Wick R, Angelova MI, Walde P, Luisi PL. Microinjection into giant vesicles and light microscopy investigation of enzyme-mediated vesicle transformations. Chem Biol 1996;3:105-111. •This work demonstrates the possibility of using GLs for direct visualization of the progress of a chemical reaction. Microinjection of snake venom phospholipase A2 inside a GL results in a slow and constant decrease in its size, until it eventually disappears.
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Sensitive force technique to probe molecular adhesion and structural linkages at biological interfaces
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•A GL pressurized by micropipette suction is used as a mechanical transducer. Pressure control of the tension tunes the sensitivity of force from 0.01 up to 1000 pN. This method is anticipated to be broadly applicable for the determination of molecular adhesion force
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Evans E, Ritchie K, Merkel R. Sensitive force technique to probe molecular adhesion and structural linkages at biological interfaces. Biophys J 1995;68:2580-2587. •A GL pressurized by micropipette suction is used as a mechanical transducer. Pressure control of the tension tunes the sensitivity of force from 0.01 up to 1000 pN. This method is anticipated to be broadly applicable for the determination of molecular adhesion force.
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