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Volumn 35, Issue 6 SUPPL., 1999, Pages 1356-1367

DNA probes and a receptor-binding assay for detection of Pseudo-nitzschia (Bacillariophyceae) species and domoic acid activity in cultured and natural samples

Author keywords

Diatoms; Domoic acid; Pseudo nitzschia; Receptor binding assay; rRNA probe; Sandwich hybridization; Whole cell hybridization

Indexed keywords

BACTERIUM CULTURE; BACTERIUM DETECTION; BATCH CELL CULTURE; DNA PROBE; DOMOIC ACID; HYBRIDIZATION; RECEPTOR BINDING ASSAY; RIBOSOMAL RNA; SANDWICH HYBRIDIZATION; TOXIN;

EID: 0033378906     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1529-8817.1999.3561356.x     Document Type: Article
Times cited : (114)

References (49)
  • 1
    • 0004234123 scopus 로고
    • National Academy Press, Washington, D.C., 432 pp
    • Ahmed, F. E. [Ed.] 1991. Seafood Safety. National Academy Press, Washington, D.C., 432 pp.
    • (1991) Seafood Safety
    • Ahmed, F.E.1
  • 3
    • 0001605425 scopus 로고    scopus 로고
    • Bloom dynamics and physiology and domoic acid-prodcing Pseudo-nitzschia species
    • Anderson, D. M., Hallegraeff, G. M. & Cembella, A. D. [Eds.] NATO Advanced Study Institute Series. Springer-Verlag, Heidelberg
    • Bates, S. S., Garrison, D. L. & Horner, R. A. 1998. Bloom dynamics and physiology and domoic acid-prodcing Pseudo-nitzschia species. In Anderson, D. M., Hallegraeff, G. M. & Cembella, A. D. [Eds.] The Physiological Ecology of Harmful Algal Blooms. NATO Advanced Study Institute Series. Springer-Verlag, Heidelberg, pp. 267-92.
    • (1998) The Physiological Ecology of Harmful Algal Blooms , pp. 267-292
    • Bates, S.S.1    Garrison, D.L.2    Horner, R.A.3
  • 4
    • 0003059341 scopus 로고
    • Discrimination between domoic-acid-producing and nontoxic forms of diatom Pseudo-nitzschia pungens using immunofluorescence
    • Bates, S. S., Leger, C., Keafer, B. A. & Anderson, D. M. 1993. Discrimination between domoic-acid-producing and nontoxic forms of diatom Pseudo-nitzschia pungens using immunofluorescence. Mar. Ecol. Prog. Ser. 100:185-95.
    • (1993) Mar. Ecol. Prog. Ser. , vol.100 , pp. 185-195
    • Bates, S.S.1    Leger, C.2    Keafer, B.A.3    Anderson, D.M.4
  • 5
    • 0342644530 scopus 로고    scopus 로고
    • Application of ribosomal RNA-targeted probes to detect pseudonitzschia multiseries and P. pungens in Atlantic Canadian waters
    • Bates, S. S., Scholin, C. A., Ferguson, M. & Leger, C. 1999. Application of ribosomal RNA-targeted probes to detect Pseudonitzschia multiseries and P. pungens in Atlantic Canadian waters. Can. Tech. Rep. Fish. Aquatic Sci. 2261:63-7.
    • (1999) Can. Tech. Rep. Fish. Aquatic Sci. , vol.2261 , pp. 63-67
    • Bates, S.S.1    Scholin, C.A.2    Ferguson, M.3    Leger, C.4
  • 7
    • 0028164011 scopus 로고
    • Diatom aggregates from the open ocean
    • Buck, K. R. & Chavez, F. P. 1994. Diatom aggregates from the open ocean. J. Plankton Res. 16:1449-57.
    • (1994) J. Plankton Res. , vol.16 , pp. 1449-1457
    • Buck, K.R.1    Chavez, F.P.2
  • 8
    • 0028173710 scopus 로고
    • Evaluation of affirm VP microbial identification test for Gardnerella vaginalis and Trichomonas vaginalis
    • Briselden, A. M. & Hillier, S. L. 1994. Evaluation of Affirm VP microbial identification test for Gardnerella vaginalis and Trichomonas vaginalis. J. Clin. Microbiol. 32:148-52.
    • (1994) J. Clin. Microbiol. , vol.32 , pp. 148-152
    • Briselden, A.M.1    Hillier, S.L.2
  • 10
    • 0024507744 scopus 로고
    • Phylogenetic stains: Ribosomal RNA-based probes for identification of single cells
    • Delong, E. F., Wickham, G. S. & Pace, N. R. 1989. Phylogenetic stains: ribosomal RNA-based probes for identification of single cells. Science 243:1360-3.
    • (1989) Science , vol.243 , pp. 1360-1363
    • Delong, E.F.1    Wickham, G.S.2    Pace, N.R.3
  • 11
    • 0028222640 scopus 로고
    • Genetic relatedness of toxic and nontoxic isolates of the marine pennate diatom Pseudonitzschia (Bacillariophyceae): Phylogenetic analysis of 18s rRNA sequences
    • Douglas, D. J., Landry, D. & Douglas, S. E. 1994. Genetic relatedness of toxic and nontoxic isolates of the marine pennate diatom Pseudonitzschia (Bacillariophyceae): phylogenetic analysis of 18S rRNA sequences. Nat. Toxins 2:166-74.
    • (1994) Nat. Toxins , vol.2 , pp. 166-174
    • Douglas, D.J.1    Landry, D.2    Douglas, S.E.3
  • 12
    • 0021103332 scopus 로고
    • Post-transcriptional regulation of ribosome formation in the nucleus of regenerating rat liver
    • Dudov, K. P. & Debeva, M. B. 1983. Post-transcriptional regulation of ribosome formation in the nucleus of regenerating rat liver. Biochem. J. 210:183-92.
    • (1983) Biochem. J. , vol.210 , pp. 183-192
    • Dudov, K.P.1    Debeva, M.B.2
  • 13
    • 0019002059 scopus 로고
    • Regulation of RNA metabolism in Tetrahymena pyrifromis. I. Nutritional shift-down
    • Eckert, W. A. & Kaffenberger, W. 1980. Regulation of RNA metabolism in Tetrahymena pyrifromis. I. Nutritional shift-down. Eur. J. Cell Biol. 21:53-62.
    • (1980) Eur. J. Cell Biol. , vol.21 , pp. 53-62
    • Eckert, W.A.1    Kaffenberger, W.2
  • 14
    • 0000279976 scopus 로고    scopus 로고
    • Pseudo-nitzschia species isolated from Galician waters: Toxicity, DNA content and lectin binding assay
    • Reguera, B., Blanco, J., Fernández, M. L. & Wyatt, T. [Eds.] Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Grafisant, Santiago de Compostella, Spain
    • Fraga, S., Alverez, J. M., Míguez, A., Fernández, L. M., Costas, E. & López-Rodas, V. 1998. Pseudo-nitzschia species isolated from Galician waters: toxicity, DNA content and lectin binding assay. In Reguera, B., Blanco, J., Fernández, M. L. & Wyatt, T. [Eds.] Harmful Algae. Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Grafisant, Santiago de Compostella, Spain. pp. 270-73.
    • (1998) Harmful Algae , pp. 270-273
    • Fraga, S.1    Alverez, J.M.2    Míguez, A.3    Fernández, L.M.4    Costas, E.5    López-Rodas, V.6
  • 15
    • 0001914227 scopus 로고
    • Culture of phytoplankton for feeding marine inverterbrate animals
    • Smith, W. L. & Chanely, M. H. [Eds.] Plenum, New York
    • Guillard, R. R. L. 1975. Culture of phytoplankton for feeding marine inverterbrate animals. In Smith, W. L. & Chanely, M. H. [Eds.] Culture of Marine Inverterbrate Animals. Plenum, New York, pp. 29-60.
    • (1975) Culture of Marine Inverterbrate Animals , pp. 29-60
    • Guillard, R.R.L.1
  • 16
    • 0003071492 scopus 로고
    • Nitzschia and Fragilariopis species studied in light and electron microscopes. I. Some marine species of the groups Nitzshia and Lanceolateae
    • Hasle, G. R. 1964. Nitzschia and Fragilariopis species studied in light and electron microscopes. I. some marine species of the groups Nitzshia and Lanceolateae. Skr. Nor. Vidensk-Akad. I. Mat.-Naturvidensk Kl. Ny. Ser. 16:1-46.
    • (1964) Skr. Nor. Vidensk-Akad. I. Mat.-Naturvidensk Kl. Ny. Ser. , vol.16 , pp. 1-46
    • Hasle, G.R.1
  • 17
    • 0003071490 scopus 로고
    • Nitzschia and Fragilariopsis species studied in light and electron microscopes. II. the group Pseudonitzschia
    • _ 1965. Nitzschia and Fragilariopsis species studied in light and electron microscopes. II. the group Pseudonitzschia. Skr. Nor. Vidensk-Akad. I. Mat.-Naturvidensk. Kl. Ny. Ser. 18:1-45.
    • (1965) Skr. Nor. Vidensk-Akad. I. Mat.-Naturvidensk. Kl. Ny. Ser. , vol.18 , pp. 1-45
  • 18
    • 0001003352 scopus 로고
    • The distribution of Nitzschia seriata Cleve and allied species
    • _ 1972. The distribution of Nitzschia seriata Cleve and allied species. Nova Hedwigia Beih. 39:171-90.
    • (1972) Nova Hedwigia Beih , vol.39 , pp. 171-190
  • 19
    • 0000388577 scopus 로고
    • Nomenclatural notes on marine planktonic diatoms: The family Bacillariaceae
    • _ 1993. Nomenclatural notes on marine planktonic diatoms: the family Bacillariaceae. Nova Hedwigia Beih. 106:315-22.
    • (1993) Nova Hedwigia Beih , vol.106 , pp. 315-322
  • 20
    • 0028312441 scopus 로고
    • Pseudo-nitzschia as a genus distinct from Nitzschia (Bacillariophyceae)
    • _ 1994. Pseudo-nitzschia as a genus distinct from Nitzschia (Bacillariophyceae). J. Phytol. 30:1036-9.
    • (1994) J. Phytol. , vol.30 , pp. 1036-1039
  • 21
    • 0000621376 scopus 로고
    • Pseudo-nitzschia pungens and P. multiseries (Bacillariophyceae): Nomenclatural history, morphology, and distribution
    • _ 1995. Pseudo-nitzschia pungens and P. multiseries (Bacillariophyceae): nomenclatural history, morphology, and distribution. J. Phycol. 30:428-35.
    • (1995) J. Phycol. , vol.30 , pp. 428-435
  • 22
    • 0001642127 scopus 로고
    • Diatom cleaning and mounting for light and electron microscopy
    • Hasle, G. R. & Fryxell, G. A. 1970. Diatom cleaning and mounting for light and electron microscopy. Am. Microsc. Soc. Trans. 89: 460-74.
    • (1970) Am. Microsc. Soc. Trans. , vol.89 , pp. 460-474
    • Hasle, G.R.1    Fryxell, G.A.2
  • 23
    • 0029749953 scopus 로고    scopus 로고
    • A review of Pseudo-nitzschia, with special reference to the Skagerrak, North Atlantic, and adjacent waters
    • Hasle, G. R., Lange, C. B. & Syvertsen, E. E. 1996. A review of Pseudo-nitzschia, with special reference to the Skagerrak, North Atlantic, and adjacent waters. Helgol. Meeresunters. 50: 131-75.
    • (1996) Helgol. Meeresunters. , vol.50 , pp. 131-175
    • Hasle, G.R.1    Lange, C.B.2    Syvertsen, E.E.3
  • 24
    • 0343078703 scopus 로고
    • Domoic acid in western Washington waters
    • Horner, R. A. & Postel, J. R. 1983. Domoic acid in western Washington waters. J. Shellfish Res. 12:141-2.
    • (1983) J. Shellfish Res. , vol.12 , pp. 141-142
    • Horner, R.A.1    Postel, J.R.2
  • 25
    • 0028890050 scopus 로고
    • Vital contribution to dissolved DNA in the marine environment as determined by differential centrifugation and kingdom probing
    • Jiang, S. C. & Paul, J. H. 1995. Vital contribution to dissolved DNA in the marine environment as determined by differential centrifugation and kingdom probing. Appl. Environ. Microbiol. 61: 317-25.
    • (1995) Appl. Environ. Microbiol. , vol.61 , pp. 317-325
    • Jiang, S.C.1    Paul, J.H.2
  • 26
    • 0027338308 scopus 로고
    • Estimating growth rate of slowly growing marine bacteria from RNA content
    • Kemp, P. F., Lee, S. & LaRoche, J. 1993. Estimating growth rate of slowly growing marine bacteria from RNA content. Appl. Environ. Microbiol. 59:2594-601.
    • (1993) Appl. Environ. Microbiol. , vol.59 , pp. 2594-2601
    • Kemp, P.F.1    Lee, S.2    LaRoche, J.3
  • 28
    • 0029974564 scopus 로고    scopus 로고
    • Development and field application of a quantitative method for examining natural assemblages of protists with oligonucleotide probes
    • Lim, E. L., Caron, D. A. & DeLong, E. F. 1996. Development and field application of a quantitative method for examining natural assemblages of protists with oligonucleotide probes. Appl. Environ. Microbiol. 62:1416-23.
    • (1996) Appl. Environ. Microbiol. , vol.62 , pp. 1416-1423
    • Lim, E.L.1    Caron, D.A.2    DeLong, E.F.3
  • 29
    • 0028186309 scopus 로고
    • Domoic acid, the toxic amino acid responsible for amnesic shellfish poisoning, now in Pseudo-nitzschia seriata (Bacillariophyceae) in Europe
    • Lundholm, N., Skov, J., Pocklington, R. & Moestrup, Ø. 1994. Domoic acid, the toxic amino acid responsible for amnesic shellfish poisoning, now in Pseudo-nitzschia seriata (Bacillariophyceae) in Europe. Phycologia 33:475-8.
    • (1994) Phycologia , vol.33 , pp. 475-478
    • Lundholm, N.1    Skov, J.2    Pocklington, R.3    Moestrup, Ø.4
  • 30
    • 0000472819 scopus 로고
    • Pseudo-nitzschia pungens and P. multiseries (Bacillariophyceae): Nuclear ribosomal DNAs and species differences
    • Manhart, J. R., Fryxell, G.A., Villac, C. & Segura, L. Y. 1995. Pseudo-nitzschia pungens and P. multiseries (Bacillariophyceae): nuclear ribosomal DNAs and species differences. J. Phycol. 31: 421-7.
    • (1995) J. Phycol. , vol.31 , pp. 421-427
    • Manhart, J.R.1    Fryxell, G.A.2    Villac, C.3    Segura, L.Y.4
  • 31
    • 0029818160 scopus 로고    scopus 로고
    • Identification of cultured Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted probes
    • Miller, P. E. & Scholin, C. A. 1996. Identification of cultured Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted probes. J. Phycol. 32:646-55.
    • (1996) J. Phycol. , vol.32 , pp. 646-655
    • Miller, P.E.1    Scholin, C.A.2
  • 32
    • 0031805455 scopus 로고    scopus 로고
    • Identification and enumeration of cultured and wild Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes and filter-based whole cell hybridization
    • _ 1998. Identification and enumeration of cultured and wild Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes and filter-based whole cell hybridization. J. Phycol. 34:371-82.
    • (1998) J. Phycol. , vol.34 , pp. 371-382
  • 33
    • 0027264175 scopus 로고
    • Use of rRNA fluorescence in situ hybridization for measuring activity of single cells in young and established biofilms
    • Poulsen, L. K., Ballard, G. & Stahl, D. A. 1993. Use of rRNA fluorescence in situ hybridization for measuring activity of single cells in young and established biofilms Appl. Environ. Microbiol. 59: 1354-60.
    • (1993) Appl. Environ. Microbiol. , vol.59 , pp. 1354-1360
    • Poulsen, L.K.1    Ballard, G.2    Stahl, D.A.3
  • 34
    • 0001936052 scopus 로고    scopus 로고
    • Domoic acid producing Pseudo-nitzshia species educed by whole cell DNA probe-based and immunochemical assays
    • Reguera, B., Blanco, J., Fernández, M. L. & Wyatt, T. [Eds.] Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Grafisant, Santiago de Compostella, Spain
    • Rhodes, L., Scholin, C., Garthwaite, I., Haywood, A. J. & Thomas, A. 1998. Domoic acid producing Pseudo-nitzshia species educed by whole cell DNA probe-based and immunochemical assays. In Reguera, B., Blanco, J., Fernández, M. L. & Wyatt, T. [Eds.] Harmful Algae, Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Grafisant, Santiago de Compostella, Spain, pp. 274-7.
    • (1998) Harmful Algae , pp. 274-277
    • Rhodes, L.1    Scholin, C.2    Garthwaite, I.3    Haywood, A.J.4    Thomas, A.5
  • 35
    • 0028043883 scopus 로고
    • Assessment of the state of individual bacterial cells by hybridization with a ribosomal RNA targeted fluorescently labeled oligonucleotide probe
    • Ruimy, R., Breittmayer, V., Boivin, V. & Christen, R. 1994. Assessment of the state of individual bacterial cells by hybridization with a ribosomal RNA targeted fluorescently labeled oligonucleotide probe FEMS Microbiol. Ecol. 15:207-14.
    • (1994) FEMS Microbiol. Ecol. , vol.15 , pp. 207-214
    • Ruimy, R.1    Breittmayer, V.2    Boivin, V.3    Christen, R.4
  • 36
    • 0002936309 scopus 로고    scopus 로고
    • Development of nucleic acid probe-based diagnostics for identifying and enumerating harmful algal bloom species
    • Anderson, D. M., Hallegraeff, G. M. & Cembella A. D. [Eds.] NATO Advanced Study Institute Series. Springer-Verlag, Heidelberg
    • Scholin, C. A. 1998. Development of nucleic acid probe-based diagnostics for identifying and enumerating harmful algal bloom species. In Anderson, D. M., Hallegraeff, G. M. & Cembella A. D. [Eds.] The Physiological Ecology of Harmful Algal Blooms. NATO Advanced Study Institute Series. Springer-Verlag, Heidelberg, pp. 337-49.
    • (1998) The Physiological Ecology of Harmful Algal Blooms , pp. 337-349
    • Scholin, C.A.1
  • 37
    • 0000896288 scopus 로고    scopus 로고
    • Detection and quantification of HAB species using antibody and DNA probes: Progress to date and future research objectives
    • Reguera, B., Blanco, J., Fernandez, M. L. & Wyatt, T. [Eds.] Xunta de Galicia and Intergovernmental Oceanographic Commision of UNESCO, Grafisant, Santiago de Gompostella, Spain
    • Scholin C. A. & Anderson, D. M. 1998. Detection and quantification of HAB species using antibody and DNA probes: progress to date and future research objectives. In Reguera, B., Blanco, J., Fernandez, M. L. & Wyatt, T. [Eds.] Harmful Algae. Xunta de Galicia and Intergovernmental Oceanographic Commision of UNESCO, Grafisant, Santiago de Gompostella, Spain. pp. 253-7.
    • (1998) Harmful Algae , pp. 253-257
    • Scholin, C.A.1    Anderson, D.M.2
  • 38
    • 0000397393 scopus 로고    scopus 로고
    • Identification of Pseudo-nitzschia australis using rRNA-targeted probes in whole cell and sandwich hybridization formats
    • Scholin, C. A., Buck, K. R., Britschgi, T., Cangelosi, G. & Chavez, F. P. 1996. Identification of Pseudo-nitzschia australis using rRNA-targeted probes in whole cell and sandwich hybridization formats. Psycologia. 35:190-7.
    • (1996) Psycologia. , vol.35 , pp. 190-197
    • Scholin, C.A.1    Buck, K.R.2    Britschgi, T.3    Cangelosi, G.4    Chavez, F.P.5
  • 39
    • 33746499241 scopus 로고    scopus 로고
    • Detection and quantification of Pseudo-nitzschia australis in cultured and natural populations using LSUrRNA-targeted probes
    • Scholin, C. A., Miller, P. E., Buck, K. R. & Chavez F. P. 1997. Detection and quantification of Pseudo-nitzschia australis in cultured and natural populations using LSUrRNA-targeted probes. Limnol. Oceanogr. 42:1265-72.
    • (1997) Limnol. Oceanogr. , vol.42 , pp. 1265-1272
    • Scholin, C.A.1    Miller, P.E.2    Buck, K.R.3    Chavez, F.P.4
  • 41
    • 0003120762 scopus 로고
    • Domoic acid and amnesic shelllish poisoning - A review
    • Todd, E. C. D. 1993. Domoic acid and amnesic shelllish poisoning - a review. J. Food Protect. 56:69-83.
    • (1993) J. Food Protect. , vol.56 , pp. 69-83
    • Todd, E.C.D.1
  • 43
    • 0031081035 scopus 로고    scopus 로고
    • A microplate receptor assay for the amnesic shellfish poisoning toxin, domoic acid, utilizing a cloned glutamate receptor
    • Van Dolah, F. M., Leighfield, T. A, Haynes, B. L., Hampson, D. R. & Ramsdell, J. S. 1997. A microplate receptor assay for the amnesic shellfish poisoning toxin, domoic acid, utilizing a cloned glutamate receptor. Anal. Biochem. 245:102-5.
    • (1997) Anal. Biochem. , vol.245 , pp. 102-105
    • Van Dolah, F.M.1    Leighfield, T.A.2    Haynes, B.L.3    Hampson, D.R.4    Ramsdell, J.S.5
  • 44
    • 0025994366 scopus 로고
    • The use of oligonucleotide probes in chaotrope-based hybridization solutions
    • Van Ness, J. & Chen, L. 1991. The use of oligonucleotide probes in chaotrope-based hybridization solutions. Nucleic Acids Res. 19:5143-51.
    • (1991) Nucleic Acids Res. , vol.19 , pp. 5143-5151
    • Van Ness, J.1    Chen, L.2
  • 46
    • 0001590092 scopus 로고
    • Pseudo-nitzschia australis Frenguelli and related species from the west coast of the USA: Occurrence and domoic acid production
    • Villac, M. C., Roelke, D. L., Chavez, F. P., Cifuentes, L. A. & Fryxell, G.A. 1993. Pseudo-nitzschia australis Frenguelli and related species from the west coast of the USA: occurrence and domoic acid production. J. Shellfish Res. 12:457-65.
    • (1993) J. Shellfish Res. , vol.12 , pp. 457-465
    • Villac, M.C.1    Roelke, D.L.2    Chavez, F.P.3    Cifuentes, L.A.4    Fryxell, G.A.5
  • 48
    • 0000039173 scopus 로고
    • Domoic acid intoxication of brown pelicans (Pelecanus occidentalis) in California
    • Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M. Toxic Elsevier, New York
    • Work, T. M., Beale, A. M., Fritz, L., Quilliam, M. A., Silver, M. A., Buck, K. R. & Wright, J. L. C. 1993. Domoic acid intoxication of brown pelicans (Pelecanus occidentalis) in California. In Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M. Toxic Marine Phytoplankton. Elsevier, New York, pp. 643-9.
    • (1993) Marine Phytoplankton , pp. 643-649
    • Work, T.M.1    Beale, A.M.2    Fritz, L.3    Quilliam, M.A.4    Silver, M.A.5    Buck, K.R.6    Wright, J.L.C.7
  • 49
    • 0029859948 scopus 로고    scopus 로고
    • Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies
    • Zheng, D., Alm, E. W., Stahl, D. A. & Raskin, L. 1996. Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies. Appl. Eviron. Microbiol. 62:4504-13
    • (1996) Appl. Eviron. Microbiol. , vol.62 , pp. 4504-4513
    • Zheng, D.1    Alm, E.W.2    Stahl, D.A.3    Raskin, L.4


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