Use of a single 13C NMR resonance of glutamate for measuring oxygen consumption in tissue

American Journal of Physiology - Endocrinology and Metabolism

Volumn 277, Issue 6 40-6, 1999, Pages

  Jeffrey, F Mark H ^a,b   Reshetov, Alexander ^c   Storey, Charles J ^a   Carvalho, Rui A ^a   Sherry, A Dean ^a,d   Malloy, Craig R ^a,b  

^a Mary Nell Ralph B Rogers M ^*  (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c INTEL CORPORATION   (United States)

^d UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

13C isotopomer analysis; Citric acid cycle flux; Heart metabolism; Nuclear magnetic resonance

Indexed keywords

2 OXOGLUTARIC ACID; ACETIC ACID; GLUCOSE; GLUTAMIC ACID; OXYGEN; PYRUVIC ACID; ACETIC ACID DERIVATIVE; CARBON; PROTON;

ACCURACY; ANIMAL TISSUE; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CITRIC ACID CYCLE; CONTROLLED STUDY; HEART MUSCLE OXYGEN CONSUMPTION; MODEL; NONHUMAN; PRIORITY JOURNAL; RAT; ANIMAL; DRUG EFFECT; HEART MUSCLE; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; ORGAN CULTURE TECHNIQUE; OXYGEN CONSUMPTION; PHYSIOLOGY; REGRESSION ANALYSIS;

ACETATES; ANIMALS; CARBON ISOTOPES; CITRIC ACID CYCLE; GLUCOSE; GLUTAMIC ACID; MAGNETIC RESONANCE SPECTROSCOPY; MYOCARDIUM; ORGAN CULTURE TECHNIQUES; OXYGEN CONSUMPTION; PROTONS; PYRUVIC ACID; RATS; REGRESSION ANALYSIS;

EID: 0033372933     PISSN: 01931849     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpendo.1999.277.6.e1111     Document Type: Article

References (35)

1. 13C NMR studies in perfused rat hearts. J. Biol. Chem. 258: 13785-13794, 1983.
2. 13C NMR spectroscopy and nonlinear least squares analysis. J. Biol. Chem. 270: 7999-8008, 1995.
3. 13C-edited NMR spectroscopy. J. Cereb. Blood Flow Metab. 10: 170-179, 1990.
4. 13C]glucose. J. Neurochem. 63: 1377-1385, 1994.
5. Hindmarsh, A. C. ODEPACK, a systematized collection of ODE solvers. In: Scientfic Computing, edited by R. S. Stepleman. Amsterdam: North-Holland, 1983, p. 55-64.
6. 13C isotopomer model for estimation of anaplerotic substrate oxidation via acetyl-CoA. Am. J. Physiol. 271 (Endocrinol. Metab. 34): E788-E799, 1996.
7. Johnson, M. L. Why, when, and how biochemists should use least squares. Anal. Biochem. 206: 215-225, 1992.
8. 13C NMR measurements of hepatocellular tricarboxylic acid cycle flux. J. Biol. Chem. 273: 12187-12194, 1998.
9. Lewandowski, E. D. Nuclear magnetic resonance evaluation of metabolic and respiratory support of work load in intact rabbit hearts. Circ. Res. 70: 576-582, 1992.
10. 13C NMR spectra of intact hearts under conditions of varied metabolite enrichment. Magn. Reson. Med. 19: 186-190, 1991.
11. Lewandowski, E. D., X. Yu, K. F. LaNoue, L. T. White, C. Doumen, and J. M. O'Donnell. Altered metabolite exchange between subcellular compartments in intact postischemic rabbit hearts. Circ. Res. 81: 165-175, 1997.
12. Lowry, O. H., and J. V. Passonneau. Acollection of metabolite assays. In: A Flexible System of Enzymatic Analysis. New York: Academic, 1972, p. 147-218.
13. Lowry, O. H., and J. V. Passonneau. Typical fluorometric procedures for metabolic assays. In: A Flexible System, of Enzymatic Analysis. New York: Academic, 1972, p. 82.
14. 2 consumption in the heart. Magma 4: 35-46, 1996.
15. 13C NMR spectroscopy. J. Biol. Chem. 263: 6964-6971, 1988.
16. 13C isotope isomers. Am. J. Physiol. 259 (Heart Circ. Physiol. 28): H987-H995, 1990.
17. 13C NMR under non-steady-state conditions. Biochemistry 29: 6756-6761, 1990.
18. 1H spectra. Magn. Reson. Med. 37: 651-657, 1997.
19. Mason, G. F., R. Gruetter, D. L. Rothman, K. L. Behar, R. G. Shulman, and E. J. Novotny. Simultaneous determination of the rates of the TCA cycle, glucose utilization, α-ketoglutarate/ glutamate exchange, and glutamine synthesis in human brain by NMR. J. Cereb. Blood Flow Metab. 15: 12-25, 1995.
20. Mason, G. F., D. L. Rothman, K. L. Behar, and R. G. Shulman. NMR determination of the TCA cycle rate and α-ketoglutarate/glutamate exchange rate in rat brain. J. Cereb. Blood Flow Metab. 12: 434-447, 1992.
21. Neider, J. A., and R. Mead. A simplex method for function minimization. Computer J. 7: 308-313, 1965.
22. Nuutinen, E. M., K. J. Peuhkurinen, E. P. Pietilainen, J. K. Hiltunen, and I. E. Hassinen. Elimination and replenishment of tricarboxylic acid-cycle intermediates in myocardium. Biochem. J. 194: 867-875, 1981.
23. O'Donnell, J. M., C. Doumen, K. F. LaNoue, L. T. White, X. Yu, N. M. Alpert, and E. D. Lewandowski. Dehydrogenase regulation of metabolite oxidation and efflux from mitochondria in intact hearts. Am. J. Physiol. 274 (Heart Circ. Physiol. 43): H467-H476, 1998.
24. Press, W. H., B. P. Flannery, S. A. Teukolsky, and W. T. Vetterling. Modeling of data. In: Numerical Recipes in C. The Art of Scientific Computing. Cambridge, UK: Cambridge Univerity Press, 1988, p. 517-565.
25. Randle, P. J., P. J. England, and R. M. Denton. Control of the tricarboxylate cycle and its interactions with glycolysis during acetate utilization in rat heart. Biochem. J. 117: 677-695, 1970.
26. 13C NMR. Magn. Reson. Med. 30: 262-266, 1993.
27. 14C-labeled citrate cycle acids. Eur. J. Biochem. 17: 350-363, 1970.
28. Sherry, A. D., P. Zhao, A. J. Wiethoff, F. M. Jeffrey, and C. R. Malloy. Effects of aminooxyacetate on glutamate compartmentation and TCA cycle kinetics in rat hearts. Am. J. Physiol. 274 (Heart Circ. Physiol. 43): H591-H599, 1998.
29. Straume, M., and M. L. Johnson. Monte Carlo method for determining complete confidence probability distributions of estimated model parameters. Methods Enzymol. 210: 117-129, 1992.
30. Weiss, R. G., S. T. Gloth, R. Kalil-Filho, V. P. Chacko, M. D. Stern, and G. Gerstenblith. Indexing tricarboxylic acid cycle flux in intact hearts by carbon-13 nuclear magnetic resonance. Circ. Res. 70: 392-408, 1992.
31. Weiss, R. G., R. Kalil-Filho, A. Herskowitz, V. P. Chacko, M. Litt, M. D. Stern, and G. Gerstenblith. Tricarboxylic acid cycle activity in postischemic rat hearts. Circulation 87: 270-282, 1993.
32. 13C-glutamate labelling by the tricarboxylic acid cycle in intact rat hearts. Biochim. Biophys. Acta 1243: 543-548, 1995.
33. 13C NMR spectra: theoretical analysis and practical considerations. Am. J. Physiol. 272 (Cell Physiol. 41): C2037-C4248, 1997.
34. Yu, X., L. T. White, N. M. Alpert, and E. D. Lewandowski. Subcellular metabolite transport and carbon isotope kinetics in the intramyocardial glutamate pool. Biochemistry 35: 6963-6968, 1996.
35. 13C NMR spectra: metabolic flux, regulation, and compartmentation in hearts. Biophys. J. 69: 2090-2102, 1995.