13C isotopomer model for estimation of anaplerotic substrate oxidation via acetyl-CoA

American Journal of Physiology - Endocrinology and Metabolism

Volumn 271, Issue 4 34-4, 1996, Pages

  Jeffrey, F Mark H ^a   Storey, Charles J ^b   Sherry, A Dean ^b   Malloy, Craig R ^b  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b NONE

Author keywords

anaplerosis; carbon 13 nuclear magnetic resonance; isotopomer analysis; tricarboxylic acid cycle

Indexed keywords

ACETYL COENZYME A; CARBON 13; MALIC ACID; OXALOACETIC ACID; PROPIONIC ACID; ASPARTIC ACID; CARBON; GLUTAMIC ACID; OXALOACETIC ACID DERIVATIVE; PROPIONIC ACID DERIVATIVE;

ANIMAL TISSUE; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CITRIC ACID CYCLE; CONTROLLED STUDY; ISOLATED HEART; NONHUMAN; OXIDATION; PRIORITY JOURNAL; RAT; ANIMAL; BIOLOGICAL MODEL; MALE; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OXIDATION REDUCTION REACTION; SPRAGUE DAWLEY RAT;

ACETYL COENZYME A; ANIMALS; ASPARTIC ACID; CARBON ISOTOPES; CITRIC ACID CYCLE; GLUTAMIC ACID; MAGNETIC RESONANCE SPECTROSCOPY; MALE; MODELS, BIOLOGICAL; OXALOACETATES; OXIDATION-REDUCTION; PROPIONATES; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0030265866     PISSN: 01931849     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpendo.1996.271.4.e788     Document Type: Article

References (29)

1. Bunger, R. Compartmented pyruvate in perfused working heart. Am. J. Physiol. 249 (Heart Circ. Physiol. 18): H439-H449, 1985.
2. 13C NMR spectroscopy and nonlinear least squares analysis. J. Biol. Chem. 270: 7999-8008, 1995
3. 13C NMR study. J. Biol. Chem. 269: 26025-2633, 1994.
4. Crawford, J. M., and J. J. Blum. Quantitative analysis of flux along the gluconeogenic, glycolytic and pentose phosphate pathways under reducing conditions in hepatocytes isolated from fed rats. Biochem. J. 212: 585-598, 1983.
5. Davis, E. J., and J. H. Quastel. The effects of short-chain fatty acids and starvation on the metabolism of glucose and lactate by the perfused guinea pig heart. Can. J. Biochem. 42: 1605-1621, 1964.
6. de Groot M. J., P. H. Willemsen, W. A. Coumans, M. van Bilsen, and G. J. van der Vusse. Lactate-induced stimulation of myocardial triacylglycerol turnover. Biochim. Biophys. Acta 1006: 111-115, 1989.
7. Frenkel, E. P., and R. L. Kitchens. Applicability of an enzymatic quantitation of methylmalonic, propionic, and acetic acids in normal and megaloblastic states. Blood 49: 125-137, 1977.
8. Hiltunen, J. K., and E. J. Davis. The disposition of citric acid cycle intermediates by isolated rat heart mitochondria Biochim. Biophys. Acta 678: 115-121, 1981.
9. 13C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism Trends Biochem. Sci. 16: 5-10, 1991.
10. Jessen, M. E., T. E. Kovarik, F. M. Jeffrey, A. D. Sherry, C. J. Storey, R. Y. Chao, W. S. Ring, and C. R. Malloy. Effects of amino acids on substrate selection, anaplerosis, and left ventricular function in the ischemic reperfused rat heart. J. Clin. Invest. 92: 831-839, 1993.
11. 13C isotopomers. Biochemistry 32: 12240-12244, 1993.
12. 14C-labeled substrates. Am. J. Physiol. 248 (Regulatory Integrative Comp. Physiol. 17): R391-R399, 1985.
13. Katz, J., and N. Grunnet. Estimation of metabolic pathways in steady state in vitro. Rates of tricarboxylic acid and pentose cycles. In: Techniques in Metabolic Research, edited by H. L. Kornberg. Amsterdam: Elsevier/North-Holland, 1979, p. 1-18.
14. 13C-labeled lactate. J. Biol. Chem. 268: 25509-25521, 1993.
15. Latipaa, P. M., K. J. Peuhkurinen, J. K. Hiltunen, and I. E. Hassinen. Regulation of pyruvate dehydrogenase during infusion of fatty acids of varying chain lengths in the perfused rat heart. J Mol. Cell. Cardiol. 17: 1161-1171, 1985.
16. Magnusson, I., W. C. Schumann, G. E. Bartsch, V. Chandramouli, K. Kumaran, J. Wahren, and B. R. Landau. Noninvasive tracing of Krebs cycle metabolism in liver. J. Biol. Chem. 266: 6975-6984, 1991.
17. 13C NMR spectroscopy. FEBS Lett. 212: 58-62, 1987.
18. 13C NMR spectroscopy. J. Biol. Chem 263: 6964-6971, 1988.
19. 13C isotope isomers. Am. J. Physiol. 259 (Heart Circ. Physiol 28): H987-H995, 1990.
20. 13C NMR under non-steady-state conditions. Biochemistry 29: 6756-6761, 1990.
21. Martin, G., M. F. Chauvin, S. Dugelay, and G. Baverel. Non-steady state model applicable to NMR studies for calculating flux rates in glycolysis, gluconeogenesis, and citric acid cycle. J. Biol. Chem. 269: 26034-26039, 1994.
22. +-dependent malic enzyme and cell renewal in Sprague-Dawley rat tissues. J. Biol. Chem 255: 3849-3854, 1980.
23. Neider, J. A., and R. Mead. A simplex method for function minimization. Comput. J. 7: 308-313, 1965.
24. Peuhkurinen, K. J. Accumulation and disposal of tricarboxylic acid cycle intermediates during propionate oxidation in the isolated perfused rat heart. Biochim. Biophys. Acta 721: 124-134, 1982.
25. Peuhkurinen, K. J., J. K. Hiltunen, and I. E. Hassinen. Metabolic compartmentation of pyruvate in the isolated perfused rat heart. Biochem. J. 210: 193-198, 1983.
26. Press, W. H., B. P. Flannery, S. A. Teukolsky, and W. T. Vetterling. Root finding and nonlinear sets of equations. In: Numerical Recipes in C. The Art of Scientific Computing. Cambridge, UK: Cambridge Univ. Press, 1988, p. 255-289.
27. Rognstad, R., and J. Katz. Gluconeogenesis in the kidney cortex. Quantitative estimation of carbon flow. J. Biol. Chem. 247: 6047-6054, 1972.
28. 13C n. m. r. spectroscopy. Biochem. J. 254: 593-598, 1988.
29. Sundqvist, K. E., K. J. Peuhkurinen, J. K. Hiltunen, and I. E. Hassinen. Effect of acetate and octanoate on tricarboxylic acid cycle metabolite disposal during propionate oxidation inthe perfused rat heart. Biochim. Biophys. Acta 801: 429-436, 1984.