Knock-out of the neural death effector domain protein PEA-15 demonstrates that its expression protects astrocytes from TNFα-induced apoptosis

Journal of Neuroscience

Volumn 19, Issue 19, 1999, Pages 8244-8251

  Kitsberg, Daniel ^d,f   Formstecher, Etienne ^a   Fauquet, Mireille ^a   Kubes, Miroslav ^a,b   Cordier, Jocelyne ^a   Canton, Brigitte ^a   Pan, GuoHua ^c,e   Rolli, Malvyne ^a   Glowinski, Jacques ^a   Chneiweiss, Hervé ^a  

^a PSL Research University   (France)

^b INSTITUTE OF VIROLOGY   (Slovakia)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

^e GENENTECH INC   (United States)

^f Alamone Laboratories   (Israel)

Author keywords

Apoptosis; Astrocytes; Caspase; Death effector domain; FADD; PEA 15; TNF

Indexed keywords

ADAPTOR PROTEIN; CASPASE; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; APOPTOSIS; ARTICLE; ASTROCYTE; CELL PROTECTION; CELL SURVIVAL; EMBRYO; GENE DELETION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION;

EID: 0033216124     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.19-19-08244.1999     Document Type: Article

References (40)

1. Alnemri ES, Livingston DJ, Nicholson DW, Salvesen G, Thornberry NA, Wong WW, Yuan J (1996) Human ICE/CED-3 protease nomenclature. Cell 87:171.
2. Araujo H, Danzinger N, Cordier J, Glowinski J, Chneiweiss H (1993) Characterization of PEA-15, a major substrate for protein kinase C in astrocytes. J Biol Chem 268:5911-5920.
3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1992) Short Protocols in Molecular Biology. New York: Greene and Wiley.
4. Becher B, D'Souza SD, Troutt AB, Antel JP (1998) Fas expression on human fetal astrocytes without susceptibility to fas-mediated cytotoxicity. Neuroscience 84:627-634.
5. Bertin J, Armstrong RC, Ottilie S, Martin DA, Wang Y, Banks S, Wang G-H, Senkevich TG, Alnemri ES, Moss B, Lenardo MJ, Tomaselli KJ, Cohen JI (1997) Death effector domain-containing herpesvirus and poxvirus proteins inhibit both Fas-and TNFR1-induced apoptosis. Proc Natl Acad Sci USA 94:1172-1176.
6. Birman S, Cordier J, Glowinski J, Chneiweiss H (1989) CyclicAMP dependent protein kinase in mouse striatal neurones and astrocytes in primary culture: development, subcellular distribution and stimulation of endogenous phosphorylation. Neurochem Int 14:25-34.
7. Boldin M, Goncharov TM, Goltsev YV, Wallach D (1996) Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO1-and TNF receptor-induced cell death. Cell 85:803-815.
8. Choi DW (1988) Glutamate neurotoxicity and diseases of the nervous system. Neuron 1:623-634.
9. Condorelli G, Vigliotta G, lavarone C, Crauso M, Tocchetti CG, Andreozzi F, Cafieri A, Tecce MF, Formisano P, Beguinot L, Beguinot F (1998) PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus. EMBO J 17:3858-3866.
10. Danziger N, Yokoyama M, Jay T, Cordier J, Glowinski J, Chneiweiss H (1995) Cellular expression, developmental regulation, and phylogenic conservation of PEA15: the major substrate for protein kinase C in astrocytes. J Neurochem 64:1016-1025.
11. Deng CX, Wynshaw-Boris A, Shen MM, Daugherty C, Ornitz DM, Leder P (1994) Murine FGFR-1 is required for early postimplantation growth and axial organization. Genes Dev 8:3045-3057.
12. Dopp J, Mackenzie-Graham A, Otero G, Merrill J (1997) Differential expression, cytokine modulation and specific functions of type-1 and type-2 tumor necrosis factor receptors in rat glia. J Neuroimmunol 75:104-112.
13. Eberstadt M, Huang B, Chen Z, Meadows RP, Ng S-C, Zheng L, Lenardo MJ, Fesik SW (1998) NMR structure and mutagenesis of the FADD(Mort1) death-effector domain. Nature 392:941-945.
14. Estelles A, Yokoyama M, Nothias F, Vincent J, Glowinski J, Vernier P, Chneiweiss H (1996) The major astrocytic phosphoprotein PEA-15 is encoded by two mRNAs conserved on their full length in mouse and human. J Biol Chem 271:14800-14806.
15. Fraser A, Evans G (1996) A license to kill. Cell 85:781-784.
16. Garrels JI (1979) Two-dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines. J Biol Chem 254:7961-7977.
17. Golstev YV, Kovalenko AV, Arnold E, Varfoloveev EE, Brodianski VM, Wallach D (1997) CASH, a novel caspase homologue with death effector domains. J Biol Chem 272:19641-19644.
18. Han DKM, Chaudhary PM, Wright M, Friedman C, Trask B, Riedel RT, Baskin DG, Schwartz ST, Hood L (1997) MRIT, a novel death-effector domain-containing protein, interacts with caspases and BclXL, and initiates cell death. Proc Natl Acad Sci USA 94:11333-11338.
19. Hu S, Vincenz C, Ni J, Gentz R, Dixit V (1997) I-FLICE, a novel inhibitor of tumor necrosis factor-1 and CD-95-induced apoptosis. J Biol Chem 272:17255-17257.
20. Inohara N, Koseki T, Hu Y, Chen S, Nunez G (1997) CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis. Proc Natl Acad Sci USA 94:10717-10722.
21. Irmler M, Thome M, Hahme M, Schneider P, Hofman K, Steiner V, Bodmer J-L, Schöter M, Burns K, Mattman C, Rimoldi D, French LE, Tschopp J (1997) Inhibition of death receptor signals by cellular FLIP. Nature 388:190-195.
22. Keane RW, Srinivasan A, Foster LM, Testa M-P, Ord T, Nonner D, Wang H-G, Reed JC, Bredesen DE, Kayalar C (1997) Activation of CPP32 during apoptosis of neurons and astrocytes. J Neurosci Res 48:168-180.
23. Kubes M, Cordier J, Glowinski J, Girault J-A, Chneiweiss H (1998) Endothelin induces a calcium-dependent phosphorylation of PEA-15 in intact astrocytes. J Neurochem 71:1307-1314.
24. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680-685.
25. Merrill JE, Benveniste ET (1996) Cytokines in inflammatory brain lesions: helpful and harmful. Trends Neurosci 19:331-338.
26. Moyse E, Michel D (1996) Analyses of apoptosis-associated DNA fragmentation in vivo during neurodegeneration in the peripheral olfactory system in adult mammals. In: Neuromethods: apoptosis (Poirier J, ed), pp 135-161. Totowa, NJ: Humana.
27. Muzio M, Chinnaiyan AM, Kischkel FC, O'Rourke K, Shevchenko A, Ni J, Scaffidi C, Bretz JD, Zhang M, Gentz R, Mann M, Krammer PH, Peter M, Dixit VM (1996) FLICE, a novel FADD-homologous ICE/ Ced3-like protease, is recruited to the CD95 (Fas/APO1) death-inducing signaling complex. Cell 85:817-827.
28. Ramos J, Kojima TJ, Hughues PE, Fenczik CA, Ginsberg M (1998) The death effector domain of PEA-15 is involved in its regulation of integrin activation. J Biol Chem 273:33897-33900.
29. Rzigalinski BA, Liang S, McKinney JS, Willoughby KA, Ellis EF (1997) Effect of Ca2+ on in vitro astrocyte injury. J Neurochem 68: 289-296.
30. Rösl F (1992) A simple and rapid method for detection of apoptosis in human cells. Nucleic Acids Res 20:5243.
31. Scaffidi C, Schmitz I, Krammer PH, Peter ME (1999) The role of c-FLIP in modulation of the CD95-induced apoptosis. J Biol Chem 274:1541-1548.
32. Shu HB, Halpin DR, Goeddel D (1997) Casper is a FADD-and caspase-related inducer of apoptosis. Immunity 6:751-763.
33. Srinivasula SM, Ahamad M, Ottilie S, Bullrich F, Banks S, Wang Y, Fernandez-Alnemri T, Croce CM, Litwak G, Tomaselli KJ, Alnemri A (1997) FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis. J Biol Chem 272:18542-18545.
34. St. Pierre BA, Merrill JE, Dopp JM (1996) Effects of cytokines on CNS cells: glia. In: Cytokines and the CNS (Merrill R, Benveniste E, eds), pp 151-168. Boca Raton: CRC.
35. Stegh AH, Schickling O, Ehret A, Scaffidi C, Peterhänsel C, Hofmann TG, Grummt I, Kramer PH, Peter ME (1998) DEDD, a novel death effector domain-containing protein, targeted to the nucleolus. EMBO J 17:5974-5986.
36. Thome M, Schneider P, Hofman K, Fickenscher H, Meinl E, Neipel F, Mattman C, Burns K, Bodmer J-L, Schöter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J (1997) Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 386:517-521.
37. Tybulewicz VL, Crawford CE, Jackson PK, Bronson RT, Mulligan RC (1991) Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogene. Cell 65:1153-1163.
38. Vincenz C, Dixit V (1997) Fas-associated death domain protein interleukin-1β-converting enzyme 2 (FLICE2), an ICE/Ced3 homologue, is proximally involved in CD95-and p55-mediated death signaling. J Biol Chem 272:6578-6583.
39. Wallach D (1997a) Cell death induction by TNF: a matter of self control. Trends Biochem Sci 22:107-109.
40. Wallach D (1997b) Placing death under control. Nature 388:123-126.