Chemical cleavage of the overexpressed mitochondrial F1β precursor with CNBr: A new strategy to construct an import-competent preprotein

Biochemical Journal

Volumn 341, Issue 1, 1999, Pages 95-103

  Pavlov, Pavel F ^a   Moberg, Per ^a   Zhang, Xiao Ping ^a   Glaser, Elzbieta ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

Avidin biotin technique; Cytochrome bc1 complex; Escherichia coli overexpression; Plant mitochondria; Processing; Protein import

Indexed keywords

ENZYME PRECURSOR; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE;

AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME DEGRADATION; ENZYME ISOLATION; ENZYME PURIFICATION; ION EXCHANGE CHROMATOGRAPHY; MITOCHONDRION; PRIORITY JOURNAL; RESPIRATORY CHAIN;

AVIDIN; BINDING, COMPETITIVE; BIOLOGICAL TRANSPORT; BIOTINYLATION; CYANOGEN BROMIDE; ELECTRON TRANSPORT COMPLEX III; ESCHERICHIA COLI; METALLOENDOPEPTIDASES; MITOCHONDRIA; PEPTIDE FRAGMENTS; PLANTS, TOXIC; PROTEIN BINDING; PROTEIN PRECURSORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTON-TRANSLOCATING ATPASES; RECOMBINANT PROTEINS; SOLANUM TUBEROSUM; SPINACIA OLERACEA; TOBACCO;

ESCHERICHIA COLI; NICOTIANA PLUMBAGINIFOLIA; SOLANUM TUBEROSUM;

EID: 0033166869     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3410095     Document Type: Article

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