Internal targeting signal of the BCS1 protein: A novel mechanism of import into mitochondria

EMBO Journal

Volumn 15, Issue 3, 1996, Pages 479-487

  Fölsch, Heike ^a   Guiard, Bernard ^b   Neupert, Walter ^a   Stuart, Rosemary A ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b CENTRE DE GÉNÉTIQUE MOLÉCULAIRE   (France)

Author keywords

BCS1; Inner membrane proteins; Membrane insertion; Mitochondrial targeting signals; Saccharomyces cerevisiae

Indexed keywords

MEMBRANE PROTEIN; RNA POLYMERASE; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; MEMBRANE TRANSPORT; MITOCHONDRIAL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING;

SACCHAROMYCES CEREVISIAE;

EID: 0030042248     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00380.x     Document Type: Article

References (29)

1. Berthold, J., Bauer, M.F., Schneider, H.C., Klaus, C., Dietmeier, K., Neupert, W. and Brunner, M. (1995) The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell, 81, 1085-1093.
2. Blom, J., Kübrich, M., Rassow, J., Voos, W., Dekker, P.J., Maarse, A.C., Meijer, M. and Pfanner, N. (1993) The essential yeast protein MIM44 (encoded by MPII) is involved in an early step of preprotein translocation across the mitochondrial inner membrane. Mol. Cell. Biol., 13, 7364-7371.
3. Blom, J., Dekker, P.J.T. and Meijer, M. (1995) Functional and physical interactions of MIM components of the yeast mitochondrial inner membrane import machinery. Eur. J. Biochem., 332, 309-314.
4. Böhni, P., Daum, G. and Schatz, G. (1983) Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in the cleavage of mitochondrial precursor proteins. J. Biol. Chem., 258, 4937-4943.
5. Boyd, D. and Beckwith, J. (1990) The role of charged amino acids in the localization of secreted and membrane proteins. Cell, 62, 1031-1033.
6. Bradford, M.M. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
7. Brunner, M., Klaus, C. and Neupert, W. (1994) The mitochondrial processing peptidase. In von Heijne, G. (ed.), Signal Peptidases. R.G. Landes, Austin, TX, pp. 73-86.
8. Cao, G. and Dalbey, R.E. (1994) Translocation of N-terminal tails across the plasma membrane. EMBO J., 13, 4662-4669.
9. 1-ATPase β subunit precursor functions as a mitochondrial import signal. J. Cell Biol., 102, 523-533.
10. Fourie, A.M., Sambrook, J.F. and Gething, M.-J.H. (1994) Common and divergent peptide binding specificities of hsp70 molecular chaperones. J. Biol. Chem., 269, 30470-30478.
11. 2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism. Cell, 69, 809-822.
12. Herrmann, J.M., Fölsch, H., Neupert, W. and Stuart, R.A. (1994) Isolation of yeast mitochondria and study of mitochondrial protein translation. In Celis, D.E. (ed.), Cell Biology: A Laboratory Handbook. Academic Press, San Diego, CA, Vol. 1, pp. 538-544.
13. Herrmann, J.M., Koll, H., Cook, R.A., Neupert, W. and Stuart, R.A. (1995) Topogenesis of cytochrome oxidase subunit II: mechanisms of protein export from the mitochondrial matrix. J. Biol. Chem., 270, 27079-27086.
14. Hochuli, E., Bannwarth, W., Döbeli, H., Gentz, R. and Stüber, D. (1988) Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology. 6, 1321-1325.
15. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
16. Maarse, A.C., Blom, J., Grivell, L.A. and Meijer, M. (1992) MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria. EMBO J., 11, 3619-3628.
17. Nobrega, F.G., Nobrega, M.P. and Tzagoloff, A. (1992) BCS1, a novel gene required for the expression of functional Rieske iron-sulfur protein in Saccharomyces cerevisiae. EMBO J., 11, 3821-3829.
18. Pelham, H.R.B. and Jackson, R.J. (1976) An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem., 67, 247-256.
19. o-ATPase subunit 9: export from the matrix requires ΔpH across the inner membrane and matrix ATP. EMBO J., 14, 3445-3451.
20. Scherer, P.E., Manning-Krieg, U., Jenö, P., Schatz, G. and Horst, M. (1992) Identification of a 45-kDa protein at the protein import site of the yeast mitochondrial inner membrane. Proc. Natl Acad. Sci. USA, 89, 11930-11934.
21. Schleyer, M., Schmidt, B. and Neupert, W. (1982) Requirement of a membrane potential for the posttranslational transfer of proteins into mitochondria. Eur. J. Biochem., 125, 109-116.
22. Schmidt, B., Wachter, E., Sebald, W. and Neupert, W. (1984) Processing peptidase of Neurospora mitochondria: Two-step cleavage of imported ATPase subunit 9. Eur. J. Biochem., 144, 581-588.
23. Schneider, H.C., Berthold, J., Bauer, M.F., Dietmeier, K., Guiard, B., Brunner, M. and Neupert, W. (1994) Mitochondrial Hsp70/MIM44 complex facilitates protein import. Nature, 371, 768-774.
24. Segui-Real, B., Kispal, G., Lill, R. and Neupert, W. (1993) Functional independence of the protein translocation machineries in mitochondrial outer and inner membranes: passage of preproteins through the inter membrane space. EMBO J., 12, 2211-2218.
25. Stuart, R.A., Cyr, D.M., Craig, E.A. and Neupert, W. (1994a) Mitochondrial molecular chaperones: their role in protein translocation. Trends Biochem. Sci., 19, 87-92.
26. Stuart, R.A., Gruhler, A., van der Klei, I., Guiard, B., Koll, H. and Neupert, W. (1994b) The requirement of matrix ATP for the import of precursor proteins into the mitochondrial matrix and intermembrane space. Eur. J. Biochem., 220, 9-18.
27. Ungermann, C., Neupert, W. and Cyr, D.M. (1994) The role of Hsp70 in conferring unidirectionality on protein translocation into mitochondria. Science, 266, 1250-1253.
28. von Heijne, G. (1986) Mitochondrial targeting sequences may form amphiphilic helices. EMBO J., 5, 1335-1342.
29. von Heijne, G. (1989) Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues. Nature, 341, 456-458.