Solution properties of the free and DNA-bound Runt domain of AML1

European Journal of Biochemistry

Volumn 261, Issue 1, 1999, Pages 251-260

  Wolf Watz, Magnus ^a   Xiao Qi, Xie ^c   Holm, Magnus ^b   Grundström, Thomas ^c   Härd, Torleif ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b YALE UNIVERSITY   (United States)

^c NONE

Author keywords

DNA complex; NMR spectroscopy; Purification; Runt domain; Unstructured N terminal

Indexed keywords

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; ELECTROPHORETIC MOBILITY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY;

BASE SEQUENCE; CIRCULAR DICHROISM; CORE BINDING FACTOR ALPHA 2 SUBUNIT; CYSTEINE; DNA; DNA PRIMERS; DNA-BINDING PROTEINS; DROSOPHILA PROTEINS; GENE EXPRESSION; HUMANS; INCLUSION BODIES; MAGNETIC RESONANCE SPECTROSCOPY; MUTAGENESIS, SITE-DIRECTED; NUCLEAR PROTEINS; OXIDATION-REDUCTION; PEPTIDE FRAGMENTS; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTO-ONCOGENE PROTEINS; RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS;

EID: 0033118989     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00269.x     Document Type: Article

References (45)

1. 1. Kagoshima, H., Akamutsu, Y., Ito, Y. & Shigesada, K. (1996) Functional dissection of the α and β subunits of transcription factor PEBP2 and the redox susceptibility of its DNA binding activity. J. Biol. Chem. 271, 33074-33082.
2. 2. Kania, M.A., Bonner, A.S., Duffy, J.B. & Gergen, J.P. (1990) The Drosophila segmentation gene runt encodes a novel nuclear regulatory protein that is also expressed in the developing nervous system. Genes Dev. 4, 1701-1713.
3. 3. Duffy, J.B., Kania, M.A. & Gergen, J.P. (1991) Expression and function of the Drosophila gene runt in early stages of neural development. Development 113, 1223-1230.
4. 4. Gergen, J.P. & Wieschaus, E.F. (1985) The localized requirements for a gene affecting segmentation in Drosophila: analysis of larvae mosaic for runt. Dev. Biol. 109, 321-335.
5. 5. Duffy, J.B. & Gergen, J.P. (1991) The Drosophila segmentation gene runt acts as a position-specific numerator element necessary for the uniform expression of the sex-determining gene sex-lethal. Genes Dev. 5, 2176-2187.
6. 6. Bae, S.C., Yamaguchi-Iwai, Y., Ogawa, E., Maruyama, M., Inuzuka, M., Kagoshima, H., Shigesada, K., Satake, M. & Ito, Y. (1993) Isolation of PEBP2αB cDNA representing the mouse homolog of human acute myeloid leukemia gene, AML1. Oncogene 8, 809-814.
7. 7. Ogawa, E., Maruyama, M., Kagoshima, H., Inuzuka, M., Lu, J., Satake, M., Shigesada, K. & Ito, Y. (1993) PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AMLI gene. Proc. Natl Acad. Sci. USA 90, 6859-6863.
8. 8. Levanon, D., Negreanu, V., Bernstein, Y., Bar-Am., I., Aviv, L. & Groner, Y. (1994) AML1, AML2, and AML3, the human members of the runt domain gene-family. cDNA structure, expression, and chromosomal localization. Genomics 23, 425-432.
9. 9. Bae, S-C., Takahashi, E., Zhang, Y.W., Ogawa, E., Shigesada, K., Namba, Y., Satake, M. & Ito, Y. (1995) Cloning mapping and expression of PEBP2αC, a third gene encoding the mammalian runt domain. Gene 159, 245-248.
10. 10. Wang, S., Wang, Q., Crute, B.E., Melinkova, I.N., Keller, S.R. & Speck, N.A. (1993) Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. Mol. Cell. Biol. 13, 3324-3339.
11. 11. Ogawa, E., Inuzuka, M., Maruyama, M., Satake, M., Naito-Fujimoto, M., Ito, Y. & Shigesada, K. (1993) Molecular cloning and characterization of PEBP2β, the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2α. Virology 194, 314-331.
12. 12. Kagoshima, H., Shiegesada, K., Satake, M., Ito, Y., Miyoshi, H., Ohki, M., Pepling, M. & Gergen, P. (1993) The runt domain identifies a new family of heteromeric transcriptional regulators. Trends Genet. 9, 338-341.
13. 13. Ducy, P., Zhang, R., Geoffroy, V., Ridall, A.L. & Karsenty, G. (1997) Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell 89, 747-754.
14. 14. Komori, T., Yagi, H., Nomura, S., Yamaguchi, A., Sasaki, K., Deguchi, K., Shimizu, Y., Bronson, R.T., Gao, Y-H., Inada, M., Sato, M., Okamoto, R., Kitamura, Y., Yoshiki, S. & Kishimoto, T. (1997) Targeted disruption of Cbfα1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89, 755-764.
15. 15. Otto, F., Thornell, A.P., Crompton, T., Denzel, A., Gilmour, K.C., Rosewell, I.R., Stamp, G.W.H., Beddington, R.S.P., Mundlos, S., Olsen, B.R., Selby, P.B. & Owen, M.J. (1997) Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell 89, 765-771.
16. 16. Liu, P., Tarle', S.A., Hajra, A., Claxton, D.F., Marlton, P., Freedman, M., Siciliano, M.J. & Collins F.S. (1993) Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia. Science 261, 1041-1044.
17. 17. Nucifora, G. & Rowley, J.D. (1995) AML1 and the 8; 21 and 3; 21 translocations in acute and chronic myeloid leukemia. Blood 86, 1-14.
18. 18. Romana, S.P., Poirel, H., Leconiat, M., Flexor, M-A., Mauchauffe', M., Jonveaux, P., Macintyre, E.A., Berger, R. & Bernard, O.A. (1995) High frequency of t(12;21) in childhood B-lineage acute lympho-blastic leukemia. Blood 86, 4263-4269.
19. 19. Shurtleff, S.A., Buijs, A., Behm, F.G., Rubnitz, J.E., Raimondi, S.C., Hancock, M.L., Chan, G.C-F., Pui, C-H., Grosveld, G. & Downing, J.R. (1995) TEL/AML1 fusion resulting from a cryptic t(12;21) is the most common genetic lesion in pediatric ALL and defines a subgroup of patients with an excellent prognosis. Leukemia 9, 1985-1989.
20. 20. Okuda, T., van Deursen, J., Hiebert, S.W., Grosveld, G. & Downing, J.R. (1996) AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis. Cell 84, 321-330.
21. 21. Wang, Q., Stacy, T., Miller, J.D., Lewis, A.F., Gu, T-L., Huang, X., Bushweller, J.H., Bories, J-C., Alt, F.W., Ryan, G., Liu, P.P., Wynshaw-Boris, A., Binder, M., Marin-Padilla, M., Sharpe, A.H. & Speck, N.A. (1996) The CBFβ subunit is essential for CBFα2 (AML1) function in vivo. Cell 87, 697-708.
22. 22. Wang, Q., Stacy, T., Binder, M., Mari'n-Padilla, M., Sharpe, A.H. & Speck, N.A. (1996) Disruption of the Cbfα2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis. Proc. Natl Acad. Sci. USA 93, 3444-3449.
23. 23. Sasaki, K., Yagi, H., Bronson, R.T., Tominaga, K., Matsunashi, T., Deguchi, K., Tani, Y., Kishimoto, T. & Komori, T. (1996) Absence of fetal liver hematopoiesis in mice deficient in transcriptional coactivator core binding factor β. Proc. Natl Acad. Sci. USA 93, 12359-12363.
24. 24. Thornell, A., Hallberg, B. & Grundström, T. (1991) Binding of SL3-3 enhancer factor 1 transcriptional activators to viral and chromosomal enhancer sequences. J. Virol. 65, 42-50.
25. 25. Thornell, A., Hallberg, B. & Grundström, T. (1988) Differential protein binding in lymphocytes to a sequence in the enhancer of the mouse retrovirus SL3-3. Mol. Cell. Biol. 8, 1625-1637.
26. 26. Melinkova, I.N., Crute, B.E., Wang, S. & Speck, N.A. (1993) Sequence specificity of the core-binding factor. J. Virol. 67, 2408-2411.
27. 27. Crute, B.E., Lewis, A.F., Wu, Z., Bushweller, J.H. & Speck, N.A. (1996) Biochemical and biophysical properties of the core-binding factor α2 (AML1) DNA-binding domain. J. Biol. Chem. 271, 26251-26260.
28. 28. Guan, K. & Dixon, J.E. (1991) Eukaryotic proteins expressed in Eschericia coli: an improved thrombin cleavage and purification procedure with glutathione S-transferase. Anal. Biochem. 192, 1-6.
29. 29. Macura, S. & Ernst, R.R. (1980) Elucidation of cross relaxation in liquids by two-dimensional NMR spectroscopy. Mol. Phys. 41, 95-117.
30. 30. States, D.J., Haberkorn, R.A. & Ruben, D.J. (1982) A two-dimensional nuclear Overhauser experiment with pure absorption phase in four quadrants. J. Magn. Reson. 48, 286-292.
31. 31. Kay, L.E., Keifer, P. & Saarinen, T. (1992) Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity. J. Am. Chem. Soc. 114, 10663-10665.
32. 32. Talluri, S. & Wagner, G. (1996) An optimized 3D NOESY-HSQC. J. Magn. Reson. 112, 200-205.
33. 33. Marion, D., Ikura, M., Tschudin, R. & Bax, A. (1989) Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteins. J. Magn. Reson. 85, 393-399.
34. 34. Johnson, W.C. Jr (1990) Protein secondary structure and circular dichroism: a practical guide. Proteins 7, 205-214.
35. 35. Levanon, D., Eisenstein, M. & Groner, Y. (1998) Site-directed mutagenesis supports a three-dimensional model of the runt domain. J. Mol. Biol. 277, 509-512.
36. 36. Davies, J.F., II, Almassay, R.J., Hostomska, Z., Ferre, R.A. & Hostomsky, Z. (1994) 2.3 Å crystal structure of the catalytic domain of DNA polymerase β. Cell 76, 1123-1133.
37. 37. Kanno, T., Kanno, Y., Chen, L-F., Ogawa, E., Kim, W-Y. & Ito, Y. (1998) Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2/core binding factor a subunit revealed in the presence of the β subunit. Mol. Cell. Biol. 18, 2444-2454.
38. 38. Hupp, T.R., Meek, D.W., Midgley, C.A. & Lane, D.P. (1992) Regulation of the specific DNA binding function of p53. Cell 71, 875-886.
39. 39. Lieberman, P.M., Schmidt, M.C., Kao, C.C. & Berk, A.J. (1991) Two distinct domains in the yeast transcription factor IID and evidence for a TATA box-induced conformational change. Mol. Cell. Biol. 11, 63-74.
40. 40. Sánchez-Garcià, I., Osada, H., Forster, A. & Rabbitts, T.H. (1993) The cysteine-rich LIM domains inhibit DNA binding by the associated homeodomain in IsI-I. EMBO 12, 4243-4250.
41. 41. Skalicky, J.J., Donaldson, L.W., Petersen, J.M., Graves, B.J. & McIntosh, L.P. (1996) Structural coupling of inhibitory regions flanking the ETS domain of murine Ets-1. Protein Sci. 5, 296-309.
42. 42. Petersen, J.M., Skalicky, J.J., Donaldson, L.W., McIntosh, L.P., Alber, T. & Graves, B.J. (1995) Modulation of transcription factor Ets-1 DNA binding: DNA-induced unfolding of an α helix. Science 269, 1866-1869.
43. 43. Wotton, D., Ghysdael, J., Wang, S., Speck, N.A. & Owen, M.J. (1994) Cooperative binding of Ets-1 and core binding factor to DNA. Mol. Cell. Biol. 14, 840-850.
44. 44. Batchelor, A.H., Piper, D.E., de Charles la Brousse, F., McKnight, S.L. & Wolberger, C. (1998) The structure of GABPα/β: an ETS domain-ankyrin repeat heterodimer bound to DNA. Science 279, 1037-1041.
45. 45. Newman, M., Strzelecka, T., Dorner, L.F., Schildkraut, I. & Aggarwal, A.K. (1995) Structure of Bam HI endonuclease bound to DNA: partial folding and unfolding on DNA binding. Science 269, 656-663.