Novel series of plasmid vectors for gene inactivation and expression analysis in group A streptococci (GAS)

Gene

Volumn 177, Issue 1-2, 1996, Pages 137-147

  Podbielski, Andreas ^a   Spellerberg, Barbara ^a   Woischnik, Markus ^a   Pohl, Barbara ^a   Lütticken, Rudolf ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Allelic replacement; DNA recombination; Mutagenesis; Reporter gene; Shuttle vector; Streptococcus pyogenes

Indexed keywords

ALKALINE PHOSPHATASE; CHLORAMPHENICOL; ERYTHROMYCIN; FLUORESCENT DYE; KANAMYCIN; RECOMBINANT DNA; SPECTINOMYCIN; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENETICS; DNA INTEGRATION; EXPRESSION VECTOR; GENE EXPRESSION; GENE REPRESSION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RECOMBINANT PLASMID; REPORTER GENE; RESTRICTION SITE; SHUTTLE VECTOR; STREPTOCOCCUS GROUP A; TRANSCRIPTION TERMINATION;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS GROUP A; STREPTOCOCCUS PYOGENES; STREPTOCOCCUS SP. 'GROUP A';

EID: 0030600488     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(96)84178-3     Document Type: Article

References (49)

1. Adams, C.W. and Hatfield, G.W. (1984) Effects of promoter strength and growth conditions on copy number of transcription-fusion vectors. J. Biol. Chem. 259, 7399-7403.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1990) Current Protocols in Molecular Biology. Greene Publishing Associates and Wiley-Interscience, New York.
3. Brehm, J., Salmond, G. and Minton, N. (1987) Sequence of the adenine methylase gene of the Streptococcus faecalis plasmid pAMβ1. Nucleic Acids Res. 15, 3177.
4. Burdett, V. (1990) Nucleotide sequence of the tet(M) gene of Tn916. Nucleic Acids Res. 18, 6137.
5. Caparon, M.G. and Scott, J.R. (1991) Genetic manipulation of pathogenic streptococci. Methods Enzymol. 204, 556-586.
6. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. and Prasher, D.C. (1994) Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
7. Chang, C.N., Kuang, Q.J. and Chen, E.Y. (1986) Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli. Gene 44, 121-125.
8. Chaussee, M.S., Gerlach, D., Yu, C.E. and Ferretti, J.J. (1993) Inactivation of the streptococcal erythrogenic toxin B gene (speB) in Streptococcus pyogenes. Infect. Immun. 61, 3719-3723.
9. Chen, J.D. and Morrison, D.A. (1987) Cloning of Streptococcus pneumoniae DNS fragments in Escherichia coli requires vectors protected by strong transcriptional terminators. Gene 55, 179-187.
10. Claverys, J.P., Dintilhac, A., Pestova, E.V., Martin, B. and Morrison, D.A. (1995) Construction and evaluation of new drug-resistance cassettes for gene disruption mutagenesis in Streptococcus pneumoniae, using an ami test platform. Gene 164, 123-128.
11. Courtney, H.S., Bronze, M.S., Dale, J.B. and Hasty, D.L. (1994) Analysis of the role of M24 protein in group A streptococcal adhesion and colonization by use of omega-interposon mutagenesis. Infect. Immun. 62, 4868-4873.
12. DeAngelis, P.L., Papaconstantinou, J. and Weigel, P.H. (1993) Isolation of a Streptococcus pyogenes gene locus that directs hyaluronan biosynthesis in acapsular mutants and in heterologous bacteria. J. Biol. Chem. 268, 14568-14571.
13. Dillard, J.P. and Yother, J. (1991) Analysis of Streptococcus pneumoniae sequences cloned into Escherichia coli: effect of promoter strength and transcription terminators. J. Bacteriol. 173, 5105-5109.
14. Fenno, J.C., Shaikh, A. and Fives-Taylor, P. (1993) Characterization of allelic replacement in Streptococcus parasanguis: transformation and homologous recombination in a 'nontransformable' streptococcus. Gene 130, 81-90.
15. Fogg, G.C., Gibson, C.M. and Caparon, M.G. (1994) The identification of rofA, a positive-acting regulatory component of prtF expression: use of an mγδ-based shuttle mutagenesis strategy in Streptococcus pyogenes. Mol. Microbiol. 11, 671-684.
16. Geist, R.T., Okada, N. and Caparon, M.G. (1993) Analysis of Streptococcus pyogenes promoters by using novel Tn916-based shuttle vectors for the construction of transcriptional fusions to chloramphenicol acetyltransferase. J. Bacteriol. 175, 7561-7570.
17. Horinouchi, S. and Weisblum, B. (1982) Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J. Bacteriol. 150, 815-825.
18. Husmann, L.K., Scott, J.R., Lindahl, G. and Stenberg, L. (1995) Expression of the Arp protein, a member of the M protein family, is not sufficient to inhibit phagocytosis of Streptococcus pyogenes. Infect. Immun. 63, 345-348.
19. Israelsen, H. and Hansen, E.B. (1993) Insertion of transposon Tn917 derivatives into the Lactococcus lactis subsp. lactis chromosome. Appl. Environ. Microbiol. 59, 21-26.
20. Ji, Y., McLandsborough, L., Kondagunta, A. and Cleary, P.P. (1996) C5a peptidase alters clearance and trafficking of group A streptococci by infected mice. Infect. Immun. 64, 503-510.
21. Kremer, L., Baulard, A., Estaquier, J., Poulain-Godefroy, O. and Locht C. (1995) Green fluorescent protein as a new expression marker in mycobacteria. Mol. Microbiol. 17, 913-922.
22. LeBlanc, D.J., Lee, L.N. and Inamine, J.M. (1991) Cloning and nuceleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Enterococcus faecalis. Antimicrob. Agent. Chemother. 35, 1804-1810.
23. Leonard, B.A.B., Podbielski, A., Hedberg, P.J. and Dunny, G.M. (1996) Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system (Opp) to import sex pheromone pCF10 for induction of conjugation. Proc. Natl. Acad. Sci. USA 93, 260-264.
24. Lovett, P.S. (1990) Translational attenuation as the regulator of inducible cat genes. J. Bacteriol. 172, 1-6.
25. Macrina, F.L., Virgili, S.S. and Scott, C.L. (1978) Extrachromosomal gene systems in Streptococcus mutans. Adv. Exp. Med. Biol. 107, 859-868.
26. McLandsborough, L.A. and Cleary, P.P. (1995) Insertional inactivation of virR in Streptococcus pyogenes M49 demonstrates that VirR functions as a positive regulator of ScpA, FcRA, OF, and M protein. FEMS Microbiol. Lett. 128, 45-52.
27. McLaughlin, R.E. and Ferretti, J.J. (1985) Electrotransformation of streptococci. In: Nickoloff, J.A. (ed.), Electroporation protocols for microorganisms. Humana Press Inc., Totowa, NJ, pp. 185-193.
28. Morrison, D.A. and Jaurin, B. (1990) Streptococcus pneumoniae possesses canonical Escherichia coli (sigma 70) promoters. Mol. Microbiol. 4, 1143-1152.
29. Norgren, M., Caparon, M.G. and Scott, J.R. (1989) A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4. Infect. Immun. 57, 3846-3850.
30. Pearce, B.J., Yin, Y.B. and Masure, H.R. (1993) Genetic identification of exported proteins in Streptococcus pneumoniae. Mol. Microbiol. 9, 1037-1050.
31. Perez-Casal, J., Caparon, M.G. and Scott, J.R. (1992) Introduction of the emm6 gene into an emm-deleted strain of Streptococcus pyogenes restores its ability to resist phagocytosis. Res. Microbiol. 143, 549-558.
32. Perez-Casal, J., Price, J.A., Maguin, E. and Scott, J.R. (1993a) An M protein with a single C repeat prevents phagocytosis of Streptococcus pyogenes: use of a temperature-sensitive shuttle vector to deliver homologous sequences to the chromosome of S. pyogenes. Mol. Microbiol. 8, 809-819.
33. Perez-Casal, J.F., Dillon, H.F., Husmann, L.K., Graham, B. and Scott, J.R. (1993b) Virulence of two Streptococcus pyogenes strains (types M1 and M3) associated with toxic-shock-like syndrome depends on an intact mry-like gene. Infect. Immun. 61, 5426-5430.
34. Perez-Casal, J., Okada, N., Caparon, M.G. and Scott, J.R. (1995) Role of the conserved C-repeat region of the M protein of Streptococcus pyogenes. Mol. Microbiol. 15, 907-916.
35. Podbielski, A., Peterson, J.A. and Cleary, P. (1992) Surface protein-CAT reporter fusions demonstrate differential gene expression in the vir regulon of Streptococcus pyogenes. Mol. Microbiol. 6, 2253-2265.
36. Podbielski, A., Flosdorff, A. and Weber-Heynemann, J. (1995) The group A streptococcal virR49 gene controls expression of four structural vir regulon genes. Infect. Immun. 63, 9-20.
37. Podbielski, A., Schnitzler, N., Beyhs, P. and Boyle, M.D.P. (1996a) M-related protein (Mrp) contributes to group A streptococcal resistance to phagocytosis by human granulocytes. Mol. Microbiol. 19, 429-441.
38. Podbielski, A., Pohl, B., Woischnik, M., Körner, C., Schmidt, K.H., Rozdzinski, E. and Leonard, B.A.B. (1996b) Molecular characterization of the group A Streptococcal (GAS) oligopeptide permease (Opp) and its effect on cysteine protease production. Mol. Microbiol. 22, in press.
39. Pozzi, G., Oggioni, M.R., Manganelli, R. and Plevani, P. (1991) Genetic manipulation of streptococci by chromosomal integration of recombinant DNA. In: Dunny, G.M., Cleary, P.P. and McKay, L.L. (Eds.), Genetics and molecular biology of streptococci, lactococci, and enterococci. American Society for Microbiology, Washington, DC, pp. 59-61.
40. Rouch, D.A., Byrne, M.E., Kong, Y.C. and Skurray, R.A. (1987) The aacA-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis. J. Gen. Microbiol. 133, 3039-3052.
41. Sabelnikov, A.G., Greenberg, B. and Lacks, S.A. (1995) An extended-10 promoter alone directs transcription of the Dpn11 operon of Streptococcus pneumoniae. J. Mol. Biol. 250, 144-155.
42. Shibl, A.M., Ramadan, M.A. and Tawfik, A.K.F. (1994) Postantibiotic effect of roxithromycin on streptolysin O production, hydrophobicity, and bactericidal activity of PMNL by Streptococcus pyogenes. Diagn. Microbiol. Infect. Dis. 20, 7-11.
43. Simon, D. and Ferretti, J.J. (1991) Electrotransformation of Streptococcus pyogenes with plasmid and linear DNA. FEMS Microbiol. Lett. 82, 219-224.
44. Steiner, K. and Malke, H. (1995) Transcription termination of the streptokinase gene: bidirectionality and efficiency in homologous and heterologous hosts. Mol. Gen. Genet. 246, 374-380.
45. Tao, L., LeBlanc, D.J. and Ferretti, J.J. (1992) Novel streptococcal-integration shuttle vectors for gene cloning and inactivation. Gene 120, 105-110.
46. Trieu-Cuot, P., Carlier, C., Poyart-Salmeron, C. and Courvalin, P. (1990) A pair of mobilizable shuttle vectors conferring resistance to spectinomycin for molecular cloning in Escherichia coli and in gram-positive bacteria. Nucleic Acids Res. 18, 4296.
47. Weisblum, B. (1995) Insights into erythromycin action from studies of its activity as inducer of resistance. Antimicrob. Agent. Chemother. 39, 797-805.
48. Wessels, M.R., Moses, A.E., Goldberg, J.B. and DiCesare, T.J. (1991) Hyaluronic acid capsule is a virulence factor for mucoid group A streptococci. Proc. Natl. Acad. Sci. USA 88, 8317-8321.
49. Zhou, C.Y., Yang, Y. and Jong, A.Y. (1990) Miniprep in ten minutes. Bio Techniques 8, 172-173.