Radiation induced chromosome aberrations and clonogenic survival in human lymphoblastoid cell lines with different p53 status

Strahlentherapie und Onkologie

Volumn 175, Issue 6, 1999, Pages 289-292

  Geiger, Constanze ^a   Weber, Klaus J ^a,b   Wenz, Frederik ^a,b,c  

^a Laboratory for Molecular and Cellular Radiobiology   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

Chromosome aberrations; Human lymphoblastoid cell lines; Irradiation; P53; TK6

Indexed keywords

DEMECOLCINE; PROTEIN P53;

APOPTOSIS; ARTICLE; CELL SURVIVAL; CHROMOSOME ABERRATION; CLONOGENESIS; CONTROLLED STUDY; GENE MUTATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; LYMPHOBLASTOID CELL; PROTEIN EXPRESSION; RADIATION INJURY; RADIOSENSITIVITY; TUMOR SUPPRESSOR GENE;

EID: 0033038562     PISSN: 01797158     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02743582     Document Type: Article

References (24)

1. Abend M, van Deuningen D. Bedeutung apoptotischer Prozesse für die Strahlentherapie. Teil I. Strahlenther Onkol 1998;174:156-66.
2. Abend M, van Beuningen D. Bedeutung apoptotischer Prozesse für die Strahlentherapie. Teil II. Strahlenther Onkol 1998;174:212-6.
3. Blank KR, Rudoltz MS, Kao CD, et al. The molecular regulation of apoptosis and implications for radiation oncology. Int J Radiat Biol 1997; 71:455-66.
4. Cornford MM, Bedford JS. A quantitative comparison of potentially lethal damage repair and the rejoining of interphase chromosome breaks in low passage normal human fibroblasts. Radiat Res 1987;111:385-405.
5. Havre PA, Yuan J, Hedrick L, et al. p53 inactivation by HPV16 E6 results in increased mutagenesis in human cells. Cancer Res 1995 5:4420-4.
6. Huang H, Li CY, Little JB. Abrogation of p53 function by transfection of HPV16 E6 gene does not enhance resistance of human tumour cells to ionizing radiation. Int J Radial Biol 1996;70:151-60.
7. Kastan MB, Onyekwere O, Sidransky D, et al. Participation of p53 protein in the cellular response to DNA damage. Cancer Res 1991;51: 6304-11.
8. Kuerbitz SJ, Plunkett BS, Walsh WF, et al. Wild-type p53 is a cell cycle checkpoint determinant following irradiation. Proc Natl Acad Sei USA 1992;89:7491-5.
9. Levy JL, Virolainen M, Defend! V. Human lymphoblastoid lines from lymph node and spleen. Cancer 1968;22:517-24.
10. Li CY, Nagasawa H, Dahlberg WK, et al. Diminished capacity for p53 in mediating a radiation-induced G, arrest in established human tumor cell lines. Oncogene 1995;! 1:1885-92.
11. Li CY, Nagasawa H, Tsang NM, et al. Radiation-induced irreversible G(0)/G(1) block is abolished in human diploid fibroblasts transfected with the human papilloma virus E6 gene: Implication of the p53-Cip1/WAFl pathway. Int J Oncol 1995;6:233-6.
12. Little JB, Nagasawa H, Keng PC, et al. Absence of radiation-induced G, arrest in 2 closely related human lymphoblastoid cell lines that differ in p53 status. J Biol Chem 1995270:11033-6.
13. Nagasawa H, Li CY, Maki CG, et al. Relationship between radiationinduced G, phase arrest and p53 function in human tumor cells. Cancer Res 1995;55:1842-6.
14. Savage JRK. Classification and relationship of induced chromosomal structural changes. J Med Genet 1975;12:103-22.
15. Scheffner M, Werness BA, Huibregtse JM, et al. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes degradation of p53. Cell 1990;63:1129-36.
16. Schwartz JL, Jordan R, Sedita BA, et al. Different sensitivity to cell killing and chromosome mutation induction by gamma rays in 2 human lymphoblastoid cell lines derived from a single donor: Possible role of apoptosis. Mutagenesis 1995;10:227-33.
17. Schwartz JL, Jordan R. Selective elimination of human lymphoid cells with unstable chromosome aberrations by p53-dependent apoptosis. Carcinogen 1997;18:201-5.
18. Slichenmyer WJ, Nelson VG, Slebos RJ, et al. Loss of a p53-associated G, checkpoint does not decrease cell survival following DNA damage. Cancer Res 1993;53:4164-8.
19. Tsang NM, Nagasawa H, Li CY, et al. Abrogation of p53 function by transfection of HPV16 E6 gene enhances the resistance of human diploid fibroblasts to ionizing radiation. Oncogene 1995;10:2403-8.
20. Wenz F, Yu Y, Nagasawa H, et al. Lack of uncoupling of S phase and mitosis in p53(-) human lymphoblastoid cell lines after irradiation. Radiat Res 1997;148:129-34.
21. Wenz F, Azzam EA, Little JB. The response of proliferating cell nuclear antigen (PCNA) to ionizing radiation is p53 dependent. Radiat Res 1998; 149:32-10.
22. Xia F, Wang X, Wang YH, et al. Altered p53 status correlates with differences in sensitivity to radiation-induced mutation and apoptosis in 2 closely related human lymphoblast lines. Cancer Res 1995:55:12-5.
23. Yu Y, Li CY, Little JB. Abrogation of p53 function by HPV16 E6 gene delays apoptosis and enhances mutagenesis but does not alter radiosensitivity in TK6 human lymphoblastoid cells. Oncogene 1997;14:1661-7.
24. Zhen W, Denault CM, Loviscek K, et al. The relative radiosensitivity of TK6 and WI-L2-NS lymphoblastoid cells derived from a common source is primarily determined by their p53 mutational status. Mutât Res 1995; 346:85-92.