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The identification of TRX2 and TRR1 as targets of both Yap1 and the Pos9/Skn7 response regulator and demonstration that Pos9/Skn7 binds the TRX2 promoter directly and independently of Yap1
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Cytoplasmic localisation of Yap1 is mediated by the Crm1 (Xpo1) nuclear export factor. The carboxy-terminal CRD sequence of Yap1 functions as a nuclear export sequence which is recognised by Crm1. The interaction between Crm1 and the CRD in vivo is reduced in response to oxidative stress.
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This paper shows that Yap1 activation occurs primarily at the level of nuclear localisation. The oxidative-stress-dependent relocalisation of Yap1 from the cytoplasm to the nucleus was found to be dependent on the carboxy-terminal CRD. Moreover, the CRD is sufficient to impart regulated nuclear localisation to a heterologous transcription factor.
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This paper shows that two different segments of Yap1 are required for oxidative-stress-dependent transcriptional activation and that these two domains function differentially depending on the nature of the oxidant used to generate the stress.
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