Regulation of connexin32 and connexin43 gene expression by DNA methylation in rat liver cells

Carcinogenesis

Volumn 20, Issue 3, 1999, Pages 401-406

  Piechocki, Marie P ^a   Burk, Robert D ^b   Ruch, Randall J ^a  

^a MEDICAL COLLEGE OF OHIO   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONNEXIN 32; CONNEXIN 43; GAP JUNCTION PROTEIN; LUCIFERASE;

ANIMAL CELL; ARTICLE; DNA METHYLATION; DNA TRANSFECTION; EPITHELIUM CELL; GENE CONTROL; HEPATOMA CELL; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RAT; SOUTHERN BLOTTING;

EID: 0033029594     PISSN: 01433334     EISSN: None     Source Type: Journal    
DOI: 10.1093/carcin/20.3.401     Document Type: Article

References (33)

1. Loewenstein, W.R. (1979) Junctional intercellular communication and the control of growth. Biochim. Biophys. Acta, 560, 1-65.
2. Trosko, J.E. and Ruch, R.J. (1998) Cell-cell communication in carcinogencsis. Front. Biosci., 3, d208-d236.
3. Bruzzone, R., White, T.W. and Paul, D.L. (1996) Connections with connexins: the molecular basis of direct intercellular signaling. Eur. J. Biochem., 238, 1-27.
4. Paul, D.L. (1986) Molecular cloning of cDNA for rat liver gap junction protein. J. Cell Biol., 103, 123-134.
5. Zhang, J.-T. and Nicholson, B.J. (1989) Sequence and tissue distribution of a second protein of hepatic cap junctions, Cx26, as deduced from its cDNA. J. Cell Biol., 109, 3391-3401.
6. Neveu, M.J., Hully, J.R., Babcock, K.L., Hertzberg, E.L., Nicholson, B.J., Paul, D.L. and Pitot, H.C. (1994) Multiple mechanisms are responsible for altered expression of gap junction genes during oncogenesis in rat liver. J. Cell Sci., 107, 83-95.
7. Janssen-Timmen, U., Traub, O., Dermietzel, R., Rabes, H.M. and Willecke, K. (1986) Reduced number of gap junctions in rat hepatocarcinomas detected by monoclonal antibody. Cancinogenesis, 7, 1475-1482.
8. Fitzgerald, D.J., Mesnil, M., Oyamada, M., Tsuda, H., Ito, N. and Yamasaki, H. (1989) Changes in gap junction protein (connexin 32) gene expression during rat liver carcinogenesis. J. Cell Biochem., 41, 97-102.
9. Ren, P., De Feijter, A.W., Paul, D.L. and Ruch, R.J. (1994) Enhancement of liver cell gap junction protein expression by glucocorticoids. Carcinogenesis, 15, 1807-1814.
10. Matesic, D.F., Rupp, H.L., Bonney, W.J., Ruch, R.J. and Trosko, J.E. (1994) Changes in gap junction permeability, phosphorylation, and number mediated by phorbol ester and non-phorbol ester tumor promoters. Mol. Carcinogen., 10, 226-236.
11. Beyer, E.C., Paul, D.L. and Goodenough, D.A. (1987) Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J. Cell Biol., 105, 2621-2629.
12. Blin, N. and Stafford, D.W. (1976) A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res., 3, 2303-2310.
13. Miller, T., Dahl, G. and Werner, R. (1988) Structure of a gap junction gene: rat connexin-32. Biosci. Rep., 8, 455-464.
14. Bai, S., Spray, D.C. and Burk, R.D. (1993) Identification of proximal and distal regulatory elements of the rat connexin32 gene. Biochim. Biophys. Acta, 1216, 197-204.
15. Yu, W., Dahl, G. and Werner, R. (1994) The connexin43 gene is responsive to oestrogen. Proc. R. Soc. Lond. B Biol. Sci., 255, 125-132.
16. Selden, R.F. (1987) Transfection using DEAE-Dextran. In Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (eds) Current Protocols in Molecular Biology. Greene Publishing and Wiley Interscience, New York, NY, pp. 9.2.1-9.2.6.
17. Wood, K.V. (1990) Firefly luciferase: a new tool for molecular biologists. Promega Notes, 28, 1-3.
18. An, G., Hidaka, K. and Siminovitch, L. (1982) Expression of bacterial β-galactosidase in animal cells. Mol. Cell. Biol., 2, 1628-1632.
19. Neuhaus, I.M., Dahl, G. and Werner, R. (1995) Use of alternate promoters for tissue-specific expression of the gene coding for connexin32. Gene, 158, 257-262.
20. Sohl, G., Gillen, C., Bosse, F., Gleichmann, M., Muller, H.W. and Willecke, K. (1996) A second alternative transcript of the gap junction gene connexin32 is expressed in murine Schwann cells and modulated in injured sciatic nerve. Eur. J. Cell Biol., 69, 267-275.
21. Tate, P.H. and Bird, A.P. (1993) Effects of DNA methylation on DNA binding proteins and gene expression. Curr. Biol., 3, 226-231.
22. Eden, S. and Cedar, H. (1994) Role of DNA methylation in the regulation of trancription. Curr. Biol., 4, 225-259.
23. Tajima, S. and Suetake, I. (1998) Regulation and function of DNA methylation in vertebrates. J. Biochem., 123, 993-999.
24. Lu, S. and Davies, P.J.A. (1997) Regulation of the expression of the tissue transglutaminase gene by DNA methylation. Proc. Natl Acad. Sci. USA, 94, 4692-4697.
25. Okuse, K., Matsuoka, I. and Kurihara, K. (1997) Tissue-specific methylation in the essential promoter element of the tyrosine hydroxylase gene. Mol. Brain Res., 46, 197-207.
26. Merlo, A., Herman, J.G., Mao, L., Lee, D.J., Gabrielson, E., Burger, P.C., Baylin, S.B. and Sidransky, D. (1995) 5′ CpG island methylation is associated with transcriptional silencing of the tumor suppressor p16/ CDKN2/MTS1 in human cancers. Nature Med., 1, 686-692.
27. Ferguson, A.T., Lapidus, R.G., Baylin, S.B. and Davidson, N.E. (1995) Demethylation of the estrogen receptor gene in estrogen-negative breast cancer cells can reactivate estrogen receptor gene expression. Cancer Res., 55, 2279-2283.
28. Kautiainen, T.L. and Jones, P.A. (1986) DNA methyltransferase levels in tumorigenic and nontumorigenic cells in culture. J. Biol. Chem., 261, 1594-1598.
29. Laird, P.W., Jackson-Grusby, L., Fazeli, A., Dickinson, S.L., Jung, W. E., Li, E., Weinberg, R.A. and Jaenisch, R. (1995) Suppression of intestinal neoplasia by DNA hypomethylation. Cell, 81, 197-205.
30. Counts, J.L. and Goodman, J.I. (1995) Alterations in DNA methylation may play a variety of roles in carcinogenesis. Cell, 83, 13-15.
31. Umezawa, A., Yamamoto, H., Rhodes, K, Klemsz, M.J., Maki, R.A. and Oshima, R.G. (1997) Methylation of an ETS site in the intron enhancer of the keratin 18 gene participates in tissue-specific repression. Mol. Cell. Biol, 17, 4885-4894.
32. Kren, B.T., Kumar, N.M., Wang, S.-Q., Gilula, N.B. and Steer, C.J. (1993) Differential regulation of multiple gap junction transcripts and proteins during rat liver regeneration. J. Cell Biol., 123, 707-718.
33. Lefebvre, D.L., Piersanti, M., Bai, X.-H., Chen, Z.-Q. and Lye, S.J. (1995) Myometrial transcriptional regulation of the gap junction gene, connexin43. Reprod. Fertil. Dev., 7, 603-611.