Role for the leucine-responsive regulatory protein (Lrp) as a structural protein in regulating the Escherichia coli gcvTHP operon

Microbiology

Volumn 145, Issue 3, 1999, Pages 569-576

  Stauffer, Lorraine T ^a   Stauffer, George V ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

DNA bending; Gcv operon; GcvA; IHF; Lrp

Indexed keywords

BACTERIAL PROTEIN; GLYCINE CLEAVAGE SYSTEM; REGULATOR PROTEIN; STRUCTURAL PROTEIN;

ARTICLE; ESCHERICHIA COLI; GENE CONTROL; NONHUMAN; OPERON; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0033026251     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-145-3-569     Document Type: Article

References (39)

1. Borst, D. L., Blumenthal, R. M. & Matthews, R. G. (1996). Use of an in vivo titration method to study a global regulator: effect of varying Lrp levels on expression of gltBDF in Escherichia coli. J Bacteriol 178, 6904-6912.
2. Bracco, L., Kotlarz, D., Kolb, A., Diekmann, S. & Buc, H. (1989). Synthetic curved DNA sequences can act as transcriptional activators in Escherichia coli. EMBO J 8, 4289-4296.
3. Calvo, J. M. & Matthews, R. G. (1994). The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli. Microbiol Rev 58, 466-490.
4. Casadaban, M. J. (1976). Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol 104, 541-555.
5. Dethiollaz, S., Eichenberger, P. & Geiselmann, J. (1996). Influence of DNA geometry on transcriptional activation in Escherichia coli. EMBO J 15, 5449-5458.
6. Friedman, D. I. (1988). Integration host factor: a protein for all reasons. Cell 55, 545-554.
7. Gartenberg, M. R. & Crothers, D. M. (1991). Synthetic DNA bending sequences increase the rate of in vitro transcription initiation at the Escherichia coli lac promoter. J Mol Biol 219, 217-230.
8. Ghrist, A. C. & Stauffer, G. V. (1995a). Characterization of the Escherichia coli gcvR gene encoding a negative regulator of gcv expression. J Bacteriol 177, 4980-4984.
9. Ghrist, A. C. & Stauffer, G. V. (1995b). The Escherichia coli glycine transport system and its role in the regulation of the glycine cleavage enzyme system. Microbiology 141, 133-140.
10. Kikuchi, G. (1973). The glycine cleavage system: composition, reaction mechanism, and physiological significance. Mol Cell Biochem 1, 169-187.
11. Kilstrup, M., Meng, L. M., Neuhard, J. & Nygaard, P. (1989). Genetic evidence for a repressor of synthesis of cytosine deaminase and purine biosynthesis enzymes in Escherichia coli. J Bacteriol 171, 2124-2127.
12. Kim, J., Zwieb, C., Wu, C. & Adhya, S. (1989). Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector. Gene 85, 15-23.
13. 70 that result in defects in phage λ repressor-stimulated transcription. J Bacteriol 176, 2991-2998.
14. Lin, R., D'Ari, R. & Newman, E. B. (1992). λ placMu insertions in genes of the leucine regulon: extension of the regulon to genes not regulated by leucine. J Bacteriol 174, 1948-1955.
15. Miller, J. H. (1992). A Short Course in Bacterial Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
16. Mudd, S. H. & Cantoni, G. L. (1964). Biological transmethylation, methyl-group neogenesis and other 'one-carbon' metabolic reactions dependent upon tetrahydrofolic acid. In Comprehensive Biochemistry, pp. 1-47. Edited by M. M. Florkin & E. H. Stotz. Amsterdam: Elsevier Publishing Company.
17. Murakami, K., Owens, J. T., Belyaeva, T. A., Meares, C. F., Busby, S. J. W. & Ishihama, A. (1997). Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors. Proc Natl Acad Sci USA 94, 11274-11278.
18. Newman, E. B., Lin, R. T. & D'Ari, R. (1996). The leucine/Lrp regulon. In Escherichia coli and Salmonella: Cellular and Molecular Biology, pp. 1513-1525. Edited by F. C. Neidhardt and others. Washington, DC: American Society for Microbiology.
19. Panasenko, S. M., Cameron, J. R., Davis, R. W. & Lehman, I. R. (1977). Five hundredfold overproduction of DNA ligase after induction of a hybrid lambda lysogen constructed in vitro. Science 196, 188-189.
20. Perez-Martin, J. & de Lorenzo, V. (1997). Clues and consequences of DNA bending in transcription. Annu Rev Microbiol 51, 593-628.
21. Plamann, M. D., Rapp, W. D. & Stauffer, G. V. (1983). Escherichia coli K12 mutants defective in the glycine cleavage enzyme system. Mol Gen Genet 192, 15-20.
22. Platko, J. V., Willins, D. A. & Calvo, J. M. (1990). The ilvIH operon of Escherichia coli is positively regulated. J Bacteriol 172, 4563-4570.
23. Rolfes, R. J. & Zalkin, H. (1988). Escherichia coli gene purR encoding a repressor protein for purine nucleoride synthesis. J Biol Chem 263, 19653-19661.
24. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
25. Sarkar, G. & Sommer, S. S. (1990). The 'megaprimer' method of site-directed mutagenesis. BioTechniques 8, 404-407.
26. Shimada, K., Weisberg, R. A. & Gottesman, M. E. (1972). Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol 63, 483-503.
27. Stauffer, L. T. & Stauffer, G. V. (1994). Characterization of the gcv control region from Escherichia coli. J Bacteriol 176, 6159-6164.
28. Stauffer, L. T. & Stauffer, G. V. (1998a). Spacing and orientation requirements of GcvA-hinding sites 3 and 2 and the Lrp-binding region for gcvT::lacZ expression in Escherichia coli. Microbiology 144, 1417-1422.
29. Stauffer, L. T. & Stauffer, G. V. (1998b). Roles for GcvA binding sites 3 and 2 and the Lrp-binding region in gcvT ::lacZ expression in Escherichia coli. Microbiology 144, 2865-2872.
30. Stauffer, L. T., Ghrist, A. & Stauffer, G. V. (1993). The Escherichia coli gcvT gene encoding the T-protein of the glycine cleavage enzyme system. DNA Seq-J DNA Seq Mapping 3, 339-346.
31. Stauffer, L. T., Fogarty, S. J. & Stauffer, G. V. (1994). Characterization of the Escherichia coli gcv operon. Gene 142, 17-22.
32. Urbanowski, M. L. & Stauffer, G. V. (1986). Autoregulation by tandem promoters of the Salmonella typhimurium LT2 met J gene. J Bacteriol 165, 740-745.
33. van der Vliet, P. C. & Verrijzer, C. P. (1993). Bending of DNA by transcription factors. BioEssays 15, 25-32.
34. Vogel, H. J. & Bonner, D. M. (1956). Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem 218, 97-106.
35. Wilson, R. L., Stauffer, L. T. & Stauffer, G. V. (1993a). Roles of the GcvA and PurR proteins in negative regulation of the Escherichia coli glycine cleavage enzyme system. J Bacteriol 175, 5129-5134.
36. Wilson, R. L., Steiert, P. S. & Stauffer, G. V. (1993b). Positive regulation of the Escherichia coli glycine cleavage enzyme system. J Bacteriol 175, 902-904.
37. Wilson, R. L., Urbanowski, M. L. & Stauffer, G. V. (1995). DNA binding sites of the LysR-type regulator GcvA in the gcv and gcvA control regions of Escherichia coli. J Bacteriol 177, 4940-4946.
38. Xin, W. & Feiss, M. (1993). Function of IHF in λ DNA packaging. I. Identification of the strong binding site for integration host factor and the locus for intrinsic bending in cosB. J Mol Biol 230, 492-504.
39. Xu, S. & Feiss, M. (1991). Structure of the bacteriophage λ cohesive end site: genetic analysis of the site (cosN) at which nicks are introduced by terminase. J Mol Biol 220, 281-292.