Spacing and orientation requirements of GcvA-binding sites 3 and 2 and the Lrp-binding region for gcvT::lacZ expression in Escherichia coli

Microbiology

Volumn 144, Issue 5, 1998, Pages 1417-1422

  Stauffer, Lorraine T ^a   Stauffer, George V ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

GcvA protein; gcvT::lacZ; Glycine cleavage; Lrp protein; Regulation

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; GLYCINE; INOSINE; RNA POLYMERASE;

ARTICLE; BACTERIAL GENE; BINDING SITE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE ACTIVATION; GENE EXPRESSION; GENE FUSION; GENE REPRESSION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PROTEIN INTERACTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0031748313     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-144-5-1417     Document Type: Article

References (26)

1. Calvo, J. M. & Matthews, R. G. (1994). The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli. Microbiol Rev 58, 466-490.
2. Casadaban, M. J. (1976). Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bactcriophage lambda and Mu. J Mol Biol 104, 541-555.
3. Ghrist, A. C. & Stauffer, G. V. (1995). Characterization of the Escherichia coli gcvR gene encoding a negative regulator of gcv expression. J Bacteriol 177, 4980-4984.
4. Kilstrup, M., Meng, L. M., Neuhard, J. & Nygaard, P. (1989). Genetic evidence for a repressor of synthesis of cytosine deaminase and purine biosynthesis enzymes in Escherichia coli. J Bacteriol 171, 2124-2127.
5. Lin, R., D'Ari, R. & Newman, E. B. (1992). λ placMu insertions in genes of the leucine regulon: extension of the regulon to genes not regulated by leucine. J Bacteriol 174, 1948-1955.
6. Miller, J. H. (1992). A Short Course in Bacterial Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
7. Mudd, S. H. & Cantoni, G. L. (1964). Biological transmethylation, methyl-group neogenesis and other 'one-carbon' metabolic reactions dependent upon tetrahydrofolic acid. In Comprehensive Biochemistry, pp. 1-47. Edited by M. Florkin & E. H. Stotz. Amsterdam: Elsevier.
8. Panasenko, S. M., Cameron, J. R., Davis, R. W. & Lehman, I. R. (1977). Five hundredfold overproduction of DNA ligase after induction of a hybrid lambda lysogen constructed in vitro. Science 196, 188-189.
9. Plamann, M. D., Rapp, W. D. & Stauffer, G. V. (1983). Escherichia coli K12 mutants defective in the glycine cleavage enzyme system. Mol Gen Genet 192, 15-20.
10. Platko, J. V., Willins, D. A. & Calvo, J. M. (1990). The ilvIH operon of Escherichia coli is positively regulated. J Bacteriol 172, 4563-4570.
11. Rolfes, R. J. & Zalkin, H. (1988). Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. J Biol Chem 263, 19653-19661.
12. Sacco, M., Ricca, E., Marasco, R., Paradiso, R. & Felice, M. D. (1993). A stereospecific alignment between the promoter and the cis-acting sequence is required for Lrp-dependent activation of ilvIH transcription in Escherichia coli. FEMS Microbiol Lett 107, 331-336.
13. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
14. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74, 5463-5467.
15. Sarkar, G. & Sommer, S. S. (1990). The 'megaprimer' method of site-directed mutagenesis. Biotechniques 8, 404-407.
16. Shimada, K., Weisberg, R. A. & Gottesman, M. E. (1972). Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol 63, 483-503.
17. Stauffer, L. T. & Stauffer, G. V. (1994). Characterization of the gcv control region from Escherichia coli. J Bacteriol 176, 6159-6164.
18. Stauffer, L. T., Ghrist, A. & Stauffer, G. V. (1993). The Escherichia coli gcvT gene encoding the T-protein of the glycine cleavage enzyme system. DNA Seq-J DNA Seq Mapping 3, 339-346.
19. Stauffer, L. T., Fogarty, S. J. & Stauffer, G. V. (1994). Characterization of the Escherichia coli gcv operon. Gene 142, 17-22.
20. Urbanowski, M. L. & Stauffer, G. V. (1986). Autoregulation by tandem promoters of the Salmonella typhimurium LT2 metJ gene. J Bacteriol 165, 740-745.
21. Vogel, H. J. & Bonner, D. M. (1956). Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem 218, 97-106.
22. Wang, Q. & Calvo, J. M. (1993). Lrp, a global regulatory protein of Escherichia coli, binds co-operatively to multiple sites and activates transcription of ilvIH. J Mol Biol 229, 306-318.
23. Wiese, D. E., Ernsting, B. R., Blumenthal, R. M. & Matthews, R. G. (1997). A nucleoprotein activation complex between the leucine-responsive regulatory protein and DNA upstream of the gltBDF operon in Escherichia coli. J Mol Biol 270, 152-168.
24. Wilson, R. L., Stauffer, L. T. & Stauffer, G. V. (1993a). Roles of the GcvA and PurR proteins in negative regulation of the Escherichia coli glycine cleavage enzyme system. J Bacteriol 175, 5129-5134.
25. Wilson, R. L., Steiert, P. S. & Stauffer, G. V. (1993b). Positive regulation of the Escherichia coli glycine cleavage enzyme system. J Bacteriol 175, 902-904.
26. Wilson, R. L., Urbanowski, M. L. & Stauffer, G. V. (1995). DNA binding sites of the LysR-type regulator GcvA in the gcv and gcvA control regions of Escherichia coli. J Bacteriol 177, 4940-4946.