Detection of Cryptosporidium parvum oocysts in faeces: Comparison of conventional coproscopical methods and the polymerase chain reaction

Veterinary Parasitology

Volumn 61, Issue 1-2, 1996, Pages 5-13

  Webster, K A ^a   Smith, H V ^b   Giles, M ^a   Dawson, L ^b   Robertson, L J ^b  

^a VETERINARY LABORATORIES AGENCY   (United Kingdom)

^b NHS GREATER GLASGOW AND CLYDE   (United Kingdom)

Author keywords

Cryptosporidium parvum; Diagnosis protozoa; Immunofluorescence; Immunomagnetic peptide separation; Polymerase chain reaction (PCR)

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; COW; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; DIAGNOSTIC ACCURACY; FECES ANALYSIS; IMMUNOFLUORESCENCE; NONHUMAN; OOCYST; POLYMERASE CHAIN REACTION;

ANIMALIA; BOVINAE; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; PROTOZOA;

EID: 0030053202     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/0304-4017(95)00811-X     Document Type: Article

References (27)

1. Allen, A.V.H. and Ridley, D.S., 1970. Further observations on the formol ether concentration technique for faecal parasites. J. Clin. Pathol., 23: 545.
2. Anusz, K.Z., Mason, P.H., Riggs, M.W. and Ferryman, L.E., 1990. Detection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme-linked immunosorbent assay. J. Clin. Microbiol., 28: 2770-2774.
3. Baxby, D., Blundell, N. and Hart, C.A., 1984. The development and performance of a simple, sensitive method for the detection of Cryptosporidium oocysts in faeces. J. Hyg., 93 (2): 317-323.
4. Bej, A.K. and Mahbubani, M.H., 1992. Applications of the polymerase chain reaction in environmental microbiology. PCR Methods Appl., 1: 151-159.
5. Casemore, D.P, Armstrong, M., Jackson, B., Nichols, G. and Thom, B.T., 1984. Screening for Cryptosporidium in stools. Lancet 1 (8379): 734-735.
6. Chapman, P.A., Rush, B.A. and McLauchlin, J., 1990. An enzyme immunoassay for detecting Cryptosporidium in faecal and environmental samples. J. Med. Microbiol., 32(4): 233-237.
7. Crawford, F.G. and Vermund, S.H., 1988. Human cryptosporidiosis. CRC Crit. Rev. Microbiol., 16 (2): 113-159.
8. Current, W.L. and Garcia, L.S., 1991. Cryptosporidiosis. Clin. Microbiol. Rev., 4: 325-358.
9. Greenson, J.K., Belitsos, P.C., Yardley, J.H. and Bartlett, J.G., 1991. AIDS enteropathy: occult enteric infections and duodenal mucosal alterations in chronic diarrhea. Ann. Intern. Med., 114: 366-372.
10. Hardingham, J.E., Kotasek, D., Farmer, B., Butler, R.N., Mi, J-X., Sage, R.E. and Dobrovic, A., 1993. Immunobead-PCR: a technique for the detection of circulating tumor cells using immunomagnetic beads and the polymerase chain reaction. Cancer Res., 53: 3455-3458.
11. Hedrum, A., Lundeberg, J., Påhlson, C. and Uhlén, M., 1992. Immunomagnetic recovery of Chlamydia trachomatis from urine with subsequent colorimetric DNA detection. PCR Methods Appl., 2: 167-171.
12. Kotler, P.D., Francisco, A., Clayton, F., Scholes, J.V. and Orenstein, J.M., 1990. Small intestinal injury and parasitic diseases in AIDS. Ann. Intern. Med., 113: 444-449.
13. Laxer, M.A., Timblin, B.K. and Patel, R.J., 1991. DNA sequences for the specific detection of Cryptosporidium parvum by the polymerase chain reaction. Am. J. Trop. Med. Hyg., 45: 688-694.
14. McLaughlin, J., Casemore, D.P., Harrison, T.B., Gerson, J., Samuel, D and Taylor, A.G., 1987. Identification of Cryptosporidium oocysts by use of monoclonal antibody. Lancet, i: 51.
15. Parker, J.F.W. and Smith, H.V., 1994. The recovery of Cryptosporidium spp. oocysts from water samples by immunomagnetic separation. Trans. R. Soc. Trop. Med Hyg., 88 (1): 25.
16. Pohlit, H.M, Paas, W. and Von Boehemar, H., 1979. Haptenisation of viable biological carriers.In: I. Lefkovits and B. Pernis (Editors), Immunological Methods. Academic Press, London, New York, pp. 181-194.
17. Robert, B., Ginter, A., Collard, A. and Coppe, P., 1990. Diagnosis of bovine cryptosporidiosis by enzyme-linked immunosorbent assay. Vet. Parasitol., 37: 1-8.
18. Roberts, W.G., Green, H.R., Ma, J., Carr, M. and Ginsberg, A.M., 1989. Prevalence of cryptosporidiosis in patients undergoing endoscopy; evidence for asymptomatic carrier state. Am. J. Med., 87: 537-539.
19. Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989. Molecular Cloning: a Laboratory Manual. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, NY.
20. Scott, C.A.., Smith, H.V. and Gibbs, H.A., 1994. Excretion of Cryptosporidium parvum in a herd of beef suckler cows. Vet. Rec., 12: 172.
21. Sheather, A.L., 1923. The detection of intestinal protozoan and mange parasites by a flotation technique. J. Comp. Pathol., 36: 266-275.
22. Tzipori, S., 1988. Cryptosporidiosis in perspective. Adv. Parasitol., 27: 63-129.
23. Tzipori, S., Angus, K.W., Gray, E.W. and Campbell, I., 1980. Vomiting and diarrhea associated with cryptosporidial infection. N. Engl. J. Med., 303: 818.
24. Ungureanu, E.M. and Dontu, G.E., 1992. A new staining technique for the identification of Cryptosporidium oocysts in faecal smears. Trans. R. Soc. Trop. Med Hyg., 86 (6): 638.
25. Weber, R., Bryan, R.T., Bishop, H.S., Wahlquist, S.P., Sullivan, J.J. and Juranek, D.D., 1991. Threshold of detection of Cryptosporidium oocysts in human stool specimens: evidence for low sensitivity in current diagnostic methods. J. Clin. Microbiol., 29: 1323-1327.
26. Weber, R., Bryan, R.T. and Juranek, D.D., 1992. Improved stool concentration procedure for the detection of Cryptosporidium oocysts in fecal specimens. J. Clin. Microbiol., 30: 2869-2873.
27. Webster, K.A., Pow, J.D.E., Giles, M., Catchpole, J. and Woodward, M.J., 1993. Detection of Cryptosporidium parvum using a specific polymerase chain reaction. Vet. Parasitol., 50: 35-44.