Atomic force microscopy of the submolecular architecture of hydrated ocular mucins

Biophysical Journal

Volumn 77, Issue 1, 1999, Pages 533-541

  McMaster, T J ^a   Berry, M ^a   Corfield, A P ^a   Miles, M J ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MUCIN;

ARTICLE; ATOMIC FORCE MICROSCOPY; EYE; HUMAN; HUMAN TISSUE; MOLECULAR DYNAMICS; PROTEIN CONFORMATION; PROTEIN GLYCOSYLATION;

EID: 0033014971     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(99)76910-9     Document Type: Article

References (51)

1. Allen, A. 1983. Mucus - a protective secretion of complexity. Trends Biochem. Sci. 8:169-173.
2. Allison, D. P., P. S. Kerper, M. J. Doktycz, J. A. Spain, M. Modrich, F. W. Larimer, T. Thundat, and R. J. Warmack. 1996. Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules. Proc. Natl. Acad. Sci. USA. 93:8826-8829.
3. Baker, A. A. 1998. High resolution AFM of polysaccharides. Ph.D. thesis. University of Bristol.
4. Baker, A. A., W. Helbert, J. Sugiyama, and M. J. Miles. 1997. High-resolution atomic force microscopy of native Valonia cellulose microcrystals. J. Struct. Biol. 119:129-138.
5. Berry, M., R. B. Ellingham, and A. P. Corfield. 1996. Polydispersity of normal human conjunctival mucins. Invest. Ophthalmol. Vis. Sci. 37: 2559-2571.
6. Bezanilla, M., S. Manne, D. E. Laney, Y. L. Lyubchenko, and H. G. Hansma. 1995. Adsorption of DNA to mica, silylated mica, and minerals - characterization by atomic force microscopy. Langmuir. 11: 655-659.
7. Buisine, M. P., J. L. Desseyn, N. Porchet, P. Degand, A. Laine, and J. P. Aubert. 1998. Genomic organization of the 3′-region of the human MUC5AC mucin gene: additional evidence for a common ancestral gene for the 11p15.5 mucin gene family. Biochem. J. 332:729-738.
8. Carlstedt, I., and J. R. Davies. 1997. Glycoconjugates facing the outside world. Biochem. Soc. Trans. 25:214-219.
9. Carlstedt, I., A. Hermann, H. Karlsson, J. Sheehan, L.-A. Fransson, and G. C. Hansson. 1993. Characterization of two different glycosylated domains from the insoluble mucin complex of rat small intestine. J. Biol. Chem. 268:18771-18781.
10. Carlstedt, I., H. Lindgren, J. K. Sheehan, U. Ulmsten, and L. Wingerup. 1983. Isolation and characterization of human cervical mucus glycoproteins. J. Biochem. 211:13-22.
11. Carlstedt, I., J. K. Sheehan, A. P. Corfield, and J. T. Gallagher. 1985. Mucous glycoproteins: a gel of a problem. Essays Biochem. 20:40-75.
12. Cherny, D. I., A. Fourcade, F. Svinarchuk, P. E. Nielsen, C. Malvy, and E. Delain. 1998. Analysis of various sequence specific triplexes by electron and atomic force microscopies. Biophys. J. 74:1015-1023.
13. Corfield, A. P., S. D. Carrington, S. J. Hicks, M. Berry, and R. B. Ellingham. 1997. Ocular mucins: purification, metabolism and function. Prog. Ret. Eye Res. 16:627-656.
14. Coury, J. E., L. McFail-Isom, L. Dean Williams, and L. A. Bottomley. 1996. A novel assay for drug-DNA binding mode, affinity and exclusion number: scanning force microscopy. Proc. Natl. Acad. Sci. USA. 93: 12283-12286.
15. Debailleul, V., A. Laine, G. Huet, P. Mathon, M. C. D'Hooghe, J. P. Aubert, and N. Porchet. 1998. Human mucin genes MUC2, MUC3, MUC4, MUC5AC, MUC5B, and MUC6 express stable and extremely large mRNAs and exhibit a variable length polymorphism. An improved method to analyze large mRNAs. J. Biol. Chem. 273:881-890.
16. Desseyn, J. L., M. P. Buisine, N. Porchet, J. P. Aubert, P. Degand, and A. Laine. 1998. Evolutionary history of the 11p15 human mucin gene family. J. Mol. Evol. 46:102-106.
17. Ellingham, R. B., M. Berry, and A. P. Corfield. 1999. Glycoforms in human ocular mucins. Glycoconj. J. (accepted for publication).
18. Fiebrig, I., S. E. Harding, A. J. Rowe, S. C. Hyman, and S. S. Davis. 1995. Transmission electron microscopy studies on pig gastric mucin and its interactions with chitosan. Carbohydr. Polym. 28:239-244.
19. Fritz, M., M. Radmacher, J. Cleveland, M. W. Allersma, R. J. Stewart, R. Gieselmann, P. Janmey, C. F. Schmidt, and P. K. Hansma. 1995. Imaging globular and filamentous proteins in physiological buffer solutions with tapping mode atomic force microscopy. Langmuir. 11: 3529-3535.
20. Gum, J. R., Jr. 1992. Mucin genes and the proteins they encode: structure, diversity and regulation. Am. J. Respir. Cell Mol. Biol. 7:557-564.
21. Guthold, M., M. Bezanilla, D. A. Erie, B. Jenkins, H. G. Hansma, and C. Bustamante. 1994. Following the assembly of RNA polymerase-DNA complexes in aqueous solutions with the scanning force microscope. Proc. Natl. Acad. Sci. USA. 91:12927-12931.
22. Hansma, H. G., and D. E. Laney. 1996. DNA binding to mica correlates with cationic radius: assay by atomic force microscopy. Biophys. J. 70:1933-1939.
23. Hicks, S. J., A. P. Corfield, R. L. Kaswan, S. Hirsh, M. Stern, J. Bara, and S. D. Carrington. 1998. Biochemical analysis of ocular surface mucin abnormalities in dry eye: the canine model. Exp. Eye Res. 67:709-718.
24. Jumel, K., F. J. J. Fogg, D. A. Mutton, J. P. Pearson, A. Allen, and S. E. Harding. 1997. A polydisperse linear random coil for the quaternary structure of pig colonic mucin. Eur. J. Biophys. 25:477-480.
25. Kasas, S., N. H. Thomson, B. L. Smith, H. G. Hansma, X. S. Zhu, M. Guthold, C. Bustamante, E. T. Kool, M. Kashlev, and P. K. Hansma. 1997. Escherichia coli RNA polymerase activity observed using atomic force microscopy. Biochemistry. 36:461-468.
26. Kirby, A. R., A. P. Gunning, and V. J. Morris. 1995. Imaging polysaccharides by atomic force microscopy. Biopolymers. 38:355-366.
27. Lyubchenko, Y. L., L. S. Shlyakhtenko, T. Aki, and S. Adhya. 1997. Atomic force microscopic demonstration of DNA looping by GalR and HU. Nucleic Acids Res. 25:873-876.
28. Maurice, D. M. 1957. The structure and transparency of the cornea. J. Physiol. (Lond.). 136:263-286.
29. McIntire, T. M., and D. A. Brant. 1997. Imaging of individual biopolymers and supramolecular assemblies using non-contact atomic force microscopy. Biopolymers. 42:133-146.
30. McIntire, T. M., and D. A. Brant. 1998. Observations of the (1-3)-β-D-glucan linear triple helix to macrocycle interconversion using non-contact atomic force microscopy. J. Am. Chem. Soc. 120:6090-6919.
31. McIntire, T. M., R. M. Penner, and D. A. Brant. 1995. Observation of a circular, triple-helical polysaccharide using non-contact atomic force microscopy. Macromolecules. 28:6375-6377.
32. McMaster, T. J., M. J. Miles, and A. E. Walsby. 1996. Direct observation of protein secondary structure in gas vesicles by atomic force microscopy. Biophys. J. 70:2432-2436.
33. Mehrotra, R., D. J. Thornton, and J. K. Sheehan. 1998. Isolation and physical characterization of the MUC7 (MG2) mucin from saliva: evidence for self-association. Biochem. J. 334:415-422.
34. Mikkelsen, A., B. T. Stokke, B. E. Christensen, and A. Elgsaeter. 1985. Flexibility and length of human bronchial mucin studied using low-shear viscometry, birefringence relaxation analysis, and electron microscopy. Biopolymers. 24:1683-1704.
35. Nollet, S., N. Moniaux, J. Maury, D. Petitprez, P. Degand, A. Laine, N. Porchet, and J. P. Aubert. 1998. Human mucin gene MUC4: organization of its 5′-region and polymorphism of its central tandem repeat array. Biochem. J. 332:739-748.
36. Rivetti, C., M. Guthold, and C. Bustamante. 1996. Scanning force microscopy of DNA deposited onto mica: equilibration versus kinetic trapping studied by statistical polymer chain analysis. J. Mol. Biol. 264:919-932.
37. Rivetti, C., C. Walker, and C. Bustamante. 1998. Polymer chain statistics and conformational analysis of DNA molecules with bends or sections of different flexibility. J. Mol. Biol. 280:41-59.
38. Roberts, C. J., A. Shivji, M. C. Davies, S. S. Davies, I. Fiebrig, S. E. Harding, S. B. Tendler, and P. M. Williams. 1995. A study of highly purified pig gastric mucin by scanning tunnelling microscopy. Protein Pept. Lett. 2:409-414.
39. Schabert, F. A., and J. P. Rabe. 1996. Vertical dimension of hydrated biological samples in tapping mode scanning force microscopy. Biophys. J. 70:1514-1520.
40. Sheehan, J. K., and I. Carlstedt. 1984. Hydrodynamic properties of human cervical mucus glycoproteins in 6 M guanidinium chloride. Biochem. J. 217:93-101.
41. Sheehan, J. K., and I. Carlstedt. 1987. Size heterogeneity of human cervical mucus glycoproteins - studies performed with rate-zonal centrifugation and laser light scattering. Biochem. J. 245:757-762.
42. Smith, T. B. 1988. Multiple scattering in the cornea. J. Modern Optics. 35:93-101.
43. Thomson, N. H., S. Kasas, B. Smith, H. G. Hansma, and P. K. 1996. Reversible binding of DNA to mica for AFM imaging. Langmuir. 12:5905-5908.
44. Thomson, N. H., M. J. Miles, S. G. Ring, P. R. Shewry, and A. S. Tatham. 1994. Real-time imaging of enzymic degradation of starch granules by atomic force microscopy. J. Vacuum Sci. Technol. 12:1565-1568.
45. Thornton, D. J., J. R. Davies, M. Kraayenbrink, P. S. Richardson, J. K. Sheehan, and I. Carlstedt. 1990. Mucus glycoproteins from "normal" human tracheobronchial secretion. Biochem. J. 265:179-186.
46. Tiffany, J. 1994. Viscoelastic properties of human tears and polymer solutions. Adv. Exp. Med. Biol. 350:267-270.
47. van Noort, S. J. T., K. O. van der Werf, A. P. M. Eker, C. Wyman, B. G. De Grooth, N. F. van Hulst, and J. Greve. 1998. Direct visualization of dynamic protein-DNA interactions with a dedicated atomic force microscope. Biophys. J. 74:2840-2849.
48. Villarrubia, J. S. 1996. Scanned probe microscope tip characterization without calibrated tip characterizers. J. Vac. Sci. Technol. B. 14: 1518-1521.
49. Vuppu, A. K., A. A. Garcia, and C. Vernia. 1997. Tapping mode atomic force microscopy of scleroglucan networks. Biopolymers. 42:89-100.
50. Xu, S., and M. F. Arnsdorf. 1997. Scanning force microscopy imaging of earthworm haemoglobin calibrated with spherical colloidal gold particles. J. Microsc. 187:43-53.
51. Yang, J., J. Mou, J.-Y. Yuan, and Z. Shao. 1996. The effect of deformation on the lateral resolution of atomic force microscopy. J. Microsc. 182: 106-113.