Detection and transcription of toxin DNA in a nontoxigenic strain of Clostridium difficile

Current Microbiology

Volumn 38, Issue 6, 1999, Pages 324-328

  Mathis, James N ^a   Pilkinton, Lynn ^a   McMillin, David E ^b  

^a UNIVERSITY OF WEST GEORGIA   (United States)

^b CLARK ATLANTA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; BACTERIAL TOXIN; MESSENGER RNA;

ARTICLE; BACTERIAL GENE; CLOSTRIDIUM DIFFICILE; CYTOTOXICITY; DNA DETERMINATION; DNA HYBRIDIZATION; DNA SEQUENCE; DNA TRANSCRIPTION; NONHUMAN; OPEN READING FRAME; PATHOGENICITY; PRIORITY JOURNAL; PSEUDOMEMBRANOUS COLITIS; SEQUENCE ANALYSIS;

BACTERIAL PROTEINS; BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; DNA, BACTERIAL; ENTEROTOXINS; GENE EXPRESSION; GENES, BACTERIAL; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

ANIMALIA; BACTERIA (MICROORGANISMS); CLOSTRIDIUM DIFFICILE; POSIBACTERIA; PROKARYOTA;

EID: 0032994027     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/PL00006811     Document Type: Article

References (34)

1. Borriello SP, Wren BW, Hyde S, Seddon SV, Sibbons P, Krishna MM, Tabaqchali S, Manek S, Price AB (1992) Molecular, immunological, and biological characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile. Infect Immun 60:4192-4199
2. Braun V, Hundsberger T, Leukel P, Sauerborn M, Eichel-Streiber Cv (1996) Definition of the single integration site of the pathogenicity locus in Clostridium difficile. Gene 181:29-38
3. Delmee M, Avesani V (1990) Virulence of ten serogroups of Clostridium difficile in hamsters. J Med Microbiol 33:85-90
4. Delmee M, Homel M, Wauters G (1985) Serogrouping of Clostridium difficile strains by slide agglutination. J Clin Microbiol 21:323-327
5. Delmee M, Avesani V, Isabelle E, Surleraux M (1990) Detection of specific antigens for ten serogroups of Clostridium difficile. Mol Cell Probes 4:1-10
6. Depitre C, Delmee M, Avensani V, L'Haridon R, Popoff M, Corthier G (1993) Serogroup F strains of Clostridium difficile produce toxin B but not toxin A. J Med Microbiol 38:434-441
7. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13-15
8. Dupuy B, Sonenshein AL (1998) Regulated transcription of Clostridium difficile toxin genes. Mol Microbiol 27:107-120
9. Ehrich M, van Tassell RL, Libby JM, Wilkins TD (1980) Production of Clostridium difficile antitoxin. Infect Immun 28:1041-1043
10. Eichel-Streiber Cv, Harperath U, Bosse D, Hadding U (1987) Purification of two high molecular mass toxins of Clostridium difficile which are antigenically related. Microb Pathog 2:307-318
11. Fluit AC, Wolfhagen MJHM, Verdonk GPHT, Jansze M, Torensma R, Verhoef J (1991) Nontoxigenic strains of Clostridium difficile lack the genes for both toxin A and toxin B. J Clin Microbiol 29:2666-2667
12. Hammond GA, Lyerly DM, Johnson JL (1997) Transcriptional analysis of the toxigenic element of Clostridium difficile. Microb Pathog 22:143-154
13. Hundsberger T, Braun V, Weidmann M, Leukel P, Sauerborn M, Eichel-Streiber Cv (1997) Transcription analysis of the genes tcdA-E of the pathogenicity locus of Clostridium difficile. Eur J Biochem 244:735-742
14. Kato N, Ou CY, Kato H, Bartley SL, Brown VK, Dowell VR, Ueno K (1991) Identification of toxigenic Clostridium difficile by the polymerase chain reaction. J Clin Microbiol 29:33-37
15. Kato H, Kato N, Watanabe K, Iwai N, Nakamura H, Yamamoto T, Suzuki K, Kim Sh-M, Chong Y, Wasito EB (1998) Identification of toxin A-negative, toxin B-positive Clostridium difficile by PCR. J Clin Microbiol 36:2178-2182
16. Knoop FC, Owens M, Crocker IC (1993) Clostridium difficile: clinical disease and diagnosis. Clin Microbiol Rev 6:251-265
17. Lyerly DM, Wilkins TD (1986) Commercial latex test for Clostridium difficile toxin A does not detect toxin A. J Clin Microbiol 23:622-623
18. Lyerly DM, Sullivan NM, Wilkins TD (1983) Enzyme-linked immunosorbent assay for Clostridium difficile toxin A. J Clin Microbiol 17:72-78
19. Lyerly DM, Saum KE, MacDonald DK, Wilkins TD (1985) Effects of Clostridium difficile toxins given intragastrically to animals. Infect Immun 47:349-352
20. Lyerly DM, Barroso LA, Wilkins TD, Depitre C, Corthier G (1992) Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile. Infect Immun 60:4633-4639
21. Mathis JN, Burkey KO (1987) Regulation of light-harvesting chlorophyll protein biosynthesis in greening seedlings: a species comparison. Plant Physiol 85:971-977
22. McMillin DE, Muldrow LL (1994) Polymerase chain reaction analysis, detection and typing of toxin producing Clostridium difficile. In: Llewellyn GC (ed) Biodeterioration Research 4. New York: Plenum Press, pp 637-648
23. McMillin DE, Muldrow LL, Laggette SJ, Abdulahi Y, Ekanemesang UM (1991) Molecular screening of Clostridium difficile toxins A and B genetic determinants and identification of mutant strains. FEMS Microbiol Lett 78:75-80
24. McMillin DE, Muldrow LL, Laggette SJ (1992) Simultaneous detection of toxin A and toxin B genetic determinants of Clostridium difficile using the multiplex polymerase chain reaction. Can J Microbiol 38:81-83
25. Moncrief JS, Barroso LA, Wilkins TD (1997) Positive regulation of Clostridium difficile toxins. Infect Immun 65:1105-1108
26. Muldrow LL, Archibold ER, Nunez-Montiel OL, Sheehy RJ (1982) Survey of the extrachromosomal gene pool of Clostridium difficile. J Clin Microbiol 16:637-640
27. Price SB, Phelps CJ, Wilkins TD, Johnson JL (1987) Cloning of the carbohydrate-binding portion of the toxin A gene of Clostridium difficile. Curr Microbiol 16:55-60
28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press
29. Virca GD, Northemann W, Shiels BR, Widera G, Broome S (1990) Simplified Northern blot hybridization using 5% sodium dodecyl sulfate. Biotechniques 8:370-371
30. Wilkins TD, Krivan H, Stiles B, Carman R, Lyerly D (1985) Clostridial toxins active locally in the gastrointestinal tract. In: Evered D, Whelan J (eds) Microbial toxins and diarrhoeal disease, London: Pitman Books Ltd, pp 230-241
31. Wren BW, Clayton CL, Mullany PP, Tabaqchali S (1987a) Molecular cloning and expression of Clostridium difficile toxin A in Escherichia coli K12. FEBS Lett 225:82-86
32. Wren BW, Heard SR, Tabaqchali S (1987b) Association between production of toxins A and B and types of Clostridium difficile. J Clin Pathol 40:1397-1401
33. Wren BW, Clayton CL, Castledine NB, Tabaqchali S (1990a) Identification of toxigenic Clostridium difficile strains by using a toxin A gene-specific probe. J Clin Microbiol 28:1808-1812
34. Wren BW, Clayton CL, Tabaqchali S (1990b) Nucleotide sequence of Clostridium difficile toxin A gene fragment and detection of toxigenic strains by polymerase chain reaction. FEMS Microbiol Lett 70:1-6