Identification of a cytoplasmic targeting/retention signal in a retroviral Gag polyprotein

Journal of Virology

Volumn 73, Issue 7, 1999, Pages 5431-5437

  Choi, Gyu ^a   Park, Sunyoung ^a   Choi, Bongkun ^a   Hong, Suntaek ^a   Lee, Jiyeon ^a   Hunter, Eric ^b   Rhee, Sung S ^a  

^a Samsung Medical Center   (South Korea)

^b University of Alabama at Birmingham   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; GAG PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MATRIX PROTEIN; TRYPTOPHAN; VIRUS PROTEIN;

AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL MEMBRANE; CYTOPLASM; IMMUNOPRECIPITATION; ISOTOPE LABELING; LEUKEMIA VIRUS; MASON PFIZER MONKEY VIRUS; MOLONEY LEUKEMIA ONCOVIRUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; RETROVIRUS; VIRUS CAPSID;

EID: 0032991707     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.73.7.5431-5437.1999     Document Type: Article

References (43)

1. Arcement, L. J., W. I. Karshin, R. B. Naso, and R. B. Arlinghaus. 1977. "Gag" polyprotein precursors of Rauscher murine leukemia virus. Virology 81:284-297.
2. Blobel, G. 1980. Intracellular protein topogenesis. Proc. Natl. Acad. Sci. USA 77:1496-1500.
3. Cannon, P. M., S. Matthews, N. Clark, E. D. Byles, O. Iourin, D. J. Hockley, S. M. Kingsman, and A. J. Kingsman. 1997. Structure-function studies of the human immunodeficiency virus type 1 matrix protein, p17. J. Virol. 71:3474-3483.
4. Chen, C., and H. Okayama. 1987. High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7:2745-2752.
5. Conte, M. R., M. Klikova, E. Hunter, T. Ruml, and S. Matthews. 1997. The three-dimensional solution structure of the matrix protein from the type D retrovirus, the Mason-Pfizer monkey virus, and implications for the morphology of retroviral assembly. EMBO J. 16:5819-5826.
6. Delchambre, M., D. Gheysen, D. Thines, C. Thiriart, E. Jacobs, E. Verdin, M. Horth, A. Burny, and F. Bex. 1989. The GAG precursor of simian immunodeficiency virus assembles into virus-like particles. EMBO J. 8:2653-2660.
7. Dingwall, C., and R. A. Laskey. 1991. Nuclear targeting sequences - a consensus? Trends Biochem. Sci. 16:478-481.
8. gag and assembly of retrovirus temperature-sensitive mutants in chronically infected cells. Virology 134:389-397.
9. gag and retrovirus assembly. Virology 130:415-426.
10. Facke, M., A. Janetzko, R. L. Shoeman, and H. G. Krausslich. 1993. A large deletion in the matrix domain of the human immunodeficiency virus gag gene redirects virus particle assembly from the plasma membrane to the endoplasmic reticulum. J. Virol. 67:4972-4980.
11. Famulari, N. G., D. L. Buchhagen, H. D. Klenk, and E. Fleissner. 1976. Presence of murine leukemia virus envelope proteins gp70 and p15(E) in a common polyprotein of infected cells. J. Virol. 20:501-508.
12. Freed, E. O., J. M. Orenstein, A. J. Buckler-White, and M. A. Martin. 1994. Single amino acid changes in the human immunodeficiency virus type 1 matrix protein block virus particle production. J. Virol. 68:5311-5320.
13. Gorlich, D. 1997. Nuclear protein import. Curr. Opin. Cell Biol. 9:412-419.
14. Gottlinger, H. G., J. G. Sodroski, and W. A. Haseltine. 1989. Role of capsid precursor processing and myristoylation in morphogenesis and infectivity of human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA 86:5781-5785.
15. Gould, S. G., G. A. Keller, and S. Subramani. 1987. Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase. J. Cell Biol. 105:2923-2931.
16. Hansen, M., L. Jelinek, R. S. Jones, J. Stegeman-Olsen, and E. Barklis. 1993. Assembly and composition of intracellular particles formed by Moloney murine leukemia virus. J. Virol. 67:5163-5174.
17. Horwich, A. 1990. Protein import into mitochondria and peroxisomes. Curr. Opin. Cell Biol. 2:625-633.
18. Kalderon, D., B. L. Roberts, W. D. Richardson, and A. E. Smith. 1984. A short amino acid sequence able to specify nuclear location. Cell 39:499-509.
19. Karshin, W. L., L. J. Arcement, R. B. Naso, and R. B. Arlinghaus. 1977. Common precursor for Rauscher leukemia virus gp69/71, p15(E) and p12(E). J. Virol. 23:787-798.
20. Klikova, M., S. S. Rhee, E. Hunter, and T. Ruml. 1995. Efficient in vivo and in vitro assembly of retroviral capsids from Gag precursor proteins expressed in bacteria. J. Virol. 69:1093-1098.
21. Lee, Y.-M., and X.-F. Yu. 1998. Identification and characterization of virus assembly intermediate complexes in HIV-1-infected CD4+ T cells. Virology 243:78-93.
22. Li, H. M., and L. J. Chen. 1997. A novel chloroplastic outer membrane-targeting signal that functions at both termini of passenger polypeptides. J. Biol. Chem. 18:10968-10974.
23. Lingappa, J. R., R. L., Hill, M. L. Wong, and R. S. Hegde. 1997. A multistep, ATP-dependent pathway for assembly of human immunodeficiency virus capsids in a cell-free system. J. Cell Biol. 136:567-581.
24. Muckel, E., and J. Soll. 1996. A protein import receptor of chloroplasts is inserted into the outer envelope membrane by a novel pathway. J. Biol. Chem. 271:23846-23852.
25. Otera, H., K. Okumoto, K. Tateishi, Y. Ikoma, E. Matsuda, M. Nishimura, T. Tsukamoto, T. Osumi, K. Ohashi, O. Higuchi, and Y. Fujiki. 1998. Peroxisome targeting signal type 1 (PTS1) receptor is involved in import of both PTS1 and PTS2: studies with PEX5-defective CHO cell mutants. Mol. Cell. Biol. 18:388-399.
26. Pain, D., H. Murakami, and G. Blobel. 1990. Identification of a receptor for protein import into mitochondria. Nature 347:444-449.
27. Pollard, V. W., W. M. Michael, B. Naldalny, M. C. Slomi, P. Wang, and G. Dreyfuss. 1996. A novel receptor mediated nuclear protein import pathway. Cell 88:985-994.
28. gag is essential for virus particle formation by Moloney murine leukemia virus. Proc. Natl. Acad. Sci. USA 83:7246-7250.
29. Rein, A., J. Mirro, J. G. Haynes, S. M. Ernst, and K. Nagashima. 1994. Function of the cytoplasmic domain of a retroviral transmembrane protein: p15E-p2E cleavage activates the membrane fusion capability of the murine leukemia virus Env protein. J. Virol. 68:1773-1781.
30. Rhee, S. S., and E. Hunter. 1991. Amino acid substitutions within the matrix protein of type D retroviruses affect assembly, transport and membrane association of a capsid. EMBO J. 10:535-546.
31. Rhee, S. S., and E. Hunter. 1987. Myristylation is required for intracellular transport but not for assembly of D-type retrovirus capsids. J. Virol. 61:1045-1053.
32. Rhee, S. S., and E. Hunter. 1990. A single amino acid substitution within the matrix protein of a type D retrovirus converts its morphogenesis to that of a type C retrovirus. Cell 63:77-86.
33. Rhee, S. S., and E. Hunter. 1990. Structural role of the matrix protein of type D retroviruses in Gag polyprotein stability and capsid assembly. J. Virol. 64:4383-4389.
34. Sabatini, D. D., G. Kreibich, T. Morimoto, and M. Adesnik. 1982. Mechanism for the incorporation of proteins in membranes and organelles. J. Cell Biol. 92:1-22.
35. Sakalian, M., S. D. Parker, R. A. Weldon, Jr., and E. Hunter. 1996. Synthesis and assembly of retrovirus Gag precursors into immature capsids in vitro. J. Virol. 70:3706-3715.
36. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5436-5467.
37. Sommerfelt, M. A., S. S. Rhee, and E. Hunter. 1992. Importance of p12 protein in Mason-Pfizer monkey virus assembly and infectivity. J. Virol. 66:7005-7011.
38. Soneoka, Y., S. M. Kingsman, and A. J. Kingsman. 1997. Mutagenesis analysis of the murine leukemia virus matrix protein: identification of regions important for membrane localization and intracellular transport. J. Virol. 71:5549-5559.
39. van Zaane, D., M. J. Dekker-Michielsen, and H. P. J. Bloemers. 1976. Virus-specific precursor polypeptides in cells infected with Rauscher leukemia virus: synthesis, identification, and processing. Virology 75:113-129.
40. Weldon, R. A., Jr., W. B. Parker, M. Sakalian, and E. Hunter. 1998. Type D retrovirus capsid assembly and release are active events requiring ATP. J. Virol. 72:3098-3106.
41. Yuan, X., X. Yu, T. H. Lee, and M. Essex. 1993. Mutations in the N-terminal region of human immunodeficiency virus type 1 matrix protein block intracellular transport of the Gag precursor. J. Virol. 67:6387-6394.
42. Zhou, W., L. J. Parent, J. W. Wills, and M. D. Resh. 1994. Identification of a membrane binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids. J. Virol. 68:2556-2569.
43. Zollar, M. J., and M. Smith. 1984. Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single stranded DNA template. DNA 3:479-488.