Post-transcriptional regulation of the Bacillus subtilis dnaK operon

Molecular Microbiology

Volumn 32, Issue 6, 1999, Pages 1183-1197

  Homuth, Georg ^a   Mogk, Axel ^a   Schumann, Wolfgang ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL RNA; HEAT SHOCK PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENETICS; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE INDUCTION; GENE MAPPING; GENETIC TRANSCRIPTION; NONHUMAN; NORTHERN BLOTTING; OPERON; PRIORITY JOURNAL; PROTEIN PROCESSING; RNA PROCESSING; RNA STRUCTURE; TRANSCRIPTION REGULATION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BINDING SITES; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; HEAT-SHOCK PROTEINS; HEATING; HSP40 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; MOLECULAR CHAPERONES; MUTAGENESIS; NUCLEIC ACID CONFORMATION; OPERATOR REGIONS (GENETICS); OPERON; REPRESSOR PROTEINS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, BACTERIAL; RNA, MESSENGER; TEMPERATURE; TRANSCRIPTION, GENETIC;

EID: 0032989815     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01428.x     Document Type: Article

References (48)

1. Belasco, J.G., Beatty, J.T., Adams, C.W., von Gabain, A., and Cohen, S.N. (1985) Differential expression of photosynthesis genes in R. capsulatus results from segmental differences in stability within the polycistronic rxcA transcript. Cell 40: 171-181.
2. Belasco, J.G., and Brawerman, G. (1993) Control of Messenger RNA Stability. New York: Academic Press.
3. Belasco, J.G., and Chen, C.-Y. (1988) Mechanism of puf mRNA degradation: the role of an intercistronic stem-loop structure. Gene 72: 109-117.
4. Bouvet, P., and Belasco, J.G. (1992) Control of RNase E-mediated RNA degradation by 5′-terminal base pairing in E. coli. Nature 360: 488-491.
5. Chen, L.-H., Emory, S.A., Bricker, A.L., Bouvet, P., and Belasco, J.G. (1991) Structure and function of a bacterial mRNA stabilizer: analysis of the 5′ untranslated region of ompA mRNA. J Bacteriol 173: 4578-4586.
6. Condon, C., Putzer, H., Luo, D., and Grunberg-Manago, M. (1997) Processing of the Bacillus subtilis thrS leader mRNA is RNase E-dependent in Escherichia coli. J Mol Biol 268: 235-242.
7. Derré, I., Rapoport, G., and Msadek, T. (1998) CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in Gram-positive bacteria. Mol Microbiol 31: 117-131.
8. Deuerling, E., Paeslack, B., and Schumann, W. (1995) The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshock. J Bacteriol 177: 4105-4112.
9. DiMari, J.F., and Bechhofer, D.H. (1993) Initiation of mRNA decay in Bacillus subtilis. Mol Microbiol 7: 705-718.
10. Emory, S.A., and Belasco, J.G. (1990) The ompA 5′ untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency. J Bacteriol 172: 4472-4481.
11. Emory, S.A., Bouvet, P., and Belasco, J.G. (1992) A 5′-terminal stem-loop structure can stabilize mRNA in Escherichia coli. Genes Dev 6: 135-148.
12. Fritsch, J., Rothfuchs, R., Rauhut, R., and Klug, G. (1995) Identification of an mRNA element promoting rate-limiting cleavage of the polycistronic puf mRNA in Rhodobacter capsulatus by an enzyme similar to RNase E. Mol Microbiol 15: 1017-1029.
13. Goldblum, K., and Apirion, D. (1981) Inactivation of the ribonucleic acid-processing enzyme ribonuclease E blocks cell division. J Bacteriol 146: 128-132.
14. B regulon. Mol Microbiol 29: 1129-1136.
15. Hecker, M., Schumann, W., and Völker, U. (1996) Heat-shock and general stress response in Bacillus subtilis. Mol Microbiol 19: 417-428.
16. Hesterkamp, T., and Bukau, B. (1998) Role of DnaK and HscA homologs of Hsp70 chaperones in protein folding in E. coli. EMBO J 17: 4818-4828.
17. Holmes, D.S., and Quigley, M. (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114: 193-197.
18. Homuth, G., Masuda, S., Mogk, A., Kobayashi, Y., and Schumann, W. (1997) The dnaK operon of Bacillus subtilis is heptacistronic. J Bacteriol 179: 1153-1164.
19. Hue, K.K., Cohen, S.D., and Bechhofer, D.H. (1995) A polypurine sequence that acts as a 5′ mRNA stabilizer in Bacillus subtilis. J Bacteriol 177: 3465-3471.
20. Jürgen, B., Schweder, T., and Hecker, M. (1998) The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet 258: 538-545.
21. Klug, G., Adams, C.W., Belasco, J.G., Doerge, B., and Cohen, S.N. (1987) Biological consequences of segmental alterations in mRNA stability: effects of deletion of the intercistronic hairpin loop region of the Rhodobacter capsulatus puf operon. EMBO J 6: 3515-3520.
22. Klug, G., and Cohen, S.N. (1990) Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic put operon mRNA. J Bacteriol 172: 140-146.
23. Klug, G., Jock, S., and Rothfuchs, R. (1992) The rate of decay of Rhodobacter capsulatus-specific puf mRNA segments is differentially affected by RNase E activity in Escherichia coli. Gene 121: 95-102.
24. Krüger, E., and Hecker, M. (1998) The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes. J Bacteriol 180: 6681-6688.
25. Kunst, F., Ogasawara, N., Moszer, I., Albertini, A.M., Alloni, G., Azevedo, V., et al. (1997) The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature 390: 249-256.
26. Mackie, G.A. (1998) Ribonuclease E is a 5′-end-dependent endonuclease. Nature 395: 720-723.
27. Mitra, S., and Bechhofer, D.H. (1994) Substrate specificity of an RNase III-like activity from Bacillus subtilis. J Biol Chem 269: 31450-31456.
28. Mogk, A., Hayward, R., and Schumann, W. (1996) Integrative vectors for constructing single-copy transcriptional fusions between Bacillus subtilis promoters and various reporter genes encoding heat-stable enzymes. Gene 182: 33-36.
29. Mogk, A., Homuth, G., Scholz, C., Kim, L., Schmid, F.X., and Schumann, W. (1997) The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis. EMBO J 16: 4579-4590.
30. Riethdorf, S., Völker, U., Gerth, U., Winkler, A., Engelmann, S., and Hecker, M. (1994) Cloning, nucleotide sequence, and expression of the Bacillus subtilis ion gene. J Bacteriol 176: 6518-6527.
31. Rowland, S.L., Katis, V.L., Partridge, S.R., and Wake, R.G. (1997) DivB, FtsZ and cell division in Bacillus subtilis. Mol Microbiol 23: 295-302.
32. Saito, H., Shibata, T., and Ando, T. (1979) Mapping of genes determining nonpermissiveness and host-specific restriction to bacteriophages in Bacillus subtilis Marburg. Mol Gen Genet 170: 117-122.
33. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
34. Schmidt, A., Schiesswohl, M., Völker, U., Hecker, M., and Schumann, W. (1992) Cloning, sequencing, mapping, and transcriptional analysis of the groESL operon from Bacillus subtilis. J Bacteriol 174: 3993-3999.
35. Schulz, A., and Schumann, W. (1996) hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat-shock genes. J Bacteriol 178: 1088-1093.
36. Schulz, A., Tzschaschel, B., and Schumann, W. (1995) Isolation and analysis of mutants of the dnaK operon of Bacillus subtilis. Mol Microbiol 15: 421-429.
37. Schulz, A., Schwab, S., Homuth, G., Versteeg, S., and Schumann, W. (1997) The htpG gene of Bacillus subtilis belongs to class III heat shock genes and is under negative control. J Bacteriol 10: 3103-3109.
38. Schumann, W., Homuth, G., and Mogk, A. (1998) The GroE chaperonin machine is the major modulator of the CIRCE heat shock regulon of Bacillus subtilis. J Biosci 23: 415-422.
39. Segal, G., and Ron, E.Z. (1996) Heat shock activation of the groESL operon of Agrobacterium tumefaciens and the regulatory roles of the inverted repeat. J Bacteriol 178: 3634-3640.
40. Stoß, O., Mogk, A., and Schumann, W. (1997) Integrative vector for constructing single-copy translational fusions between regulatory regions of Bacillus subtilis and the bgaB reporter gene encoding a heat-stable β-galactosidase. FEMS Microbiol Lett 150: 49-54.
41. Vanet, A., Plumbridge, J.A., and Alix, J.-H. (1993) Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12. J Bacteriol 175: 7178-7188.
42. Versteeg, S., Mogk, A., and Schumann, W. (1999) The Bacillus subtilis htpG gene is not involved in thermal stress management. Mol Gen Genet (in press).
43. Völker, U., Engelmann, S., Maul, B., Riethdorf, S., Völker, A., Schmid, R., et al. (1994) Analysis of the induction of general stress proteins of Bacillus subtilis. Microbiology 140: 741-752.
44. Wetzstein, M., Völker, U., Dedio, J., Löbau, S., Zuber, U., Schiesswohl, M., et al. (1992) Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J Bacteriol 174: 3300-3310.
45. Yuan, G., and Wong, S.-L. (1995a) Isolation and characterization of Bacillus subtilis regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK. J Bacteriol 177: 6462-6468.
46. A-like promoter and the roles of the inverted repeat sequence (CIRCE). J Bacteriol 177: 5427-5433.
47. Zuber, U., and Schumann, W. (1994) CIRCE, a novel heat shock element involved in regulation of heat shock operon dnaK of Bacillus subtilis. J Bacteriol 176: 1359-1363.
48. Zuker, M. (1989) On finding all suboptimal foldings of an RNA molecule. Science 244: 48-52.