Integrative vector for constructing single-copy translational fusions between regulatory regions of Bacillus subtilis and the bgaB reporter gene encoding a heat-stable β-galactosidase

FEMS Microbiology Letters

Volumn 150, Issue 1, 1997, Pages 49-54

  Stoß, Oliver ^a   Mogk, Axel ^a   Schumann, Wolfgang ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

amyE; bgaB; cat 86; heat shock; integration vector; recombinant DNA

Indexed keywords

BETA GALACTOSIDASE; CHLORAMPHENICOL; RECOMBINANT DNA;

ARTICLE; BACILLUS SUBTILIS; CLONING VECTOR; CONTROLLED STUDY; DNA SEQUENCE; GENE EXPRESSION REGULATION; GENE FUSION; HEAT SHOCK; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL;

ALPHA-AMYLASE; BACILLUS STEAROTHERMOPHILUS; BACILLUS SUBTILIS; BASE SEQUENCE; BETA-GALACTOSIDASE; BETA-LACTAMASES; CHLORAMPHENICOL; CHLORAMPHENICOL O-ACETYLTRANSFERASE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENES, REPORTER; GENETIC VECTORS; HEAT; HSP70 HEAT-SHOCK PROTEINS; KANAMYCIN KINASE; MOLECULAR SEQUENCE DATA; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID;

EID: 17044456281     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00095-5     Document Type: Article

References (23)

1. Casadaban, M.J., Chou, J. and Cohen, S.N. (1980) In vitro gene fusions that join an enzymatically active β-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J. Bacteriol. 143, 971-980.
2. Youngman, P., Zuber, P., Perkins, J.B., Sandman, K., Igo, M. and Losick, R. (1985) New ways to study developmental genes in spore-forming bacteria. Science 228, 285-291.
3. Ferrari, E., Howard, S.M.H. and Hoch, J.A. (1985) Effect of sporulation mutations assayed using a subtilisin-β-galactosidase gene fusion. In: Molecular Biology of Microbial Differentiation (Hoch, J.A. and Setlow, P., Eds.), pp. 180-184. American Society for Microbiology, Washington, DC.
4. Zuber, P. and Losick, R. (1983) Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation. Cell 35, 275-283.
5. Donelly, C.E. and Sonenshein, A.L. (1982) Genetic fusion of E. coli lac genes to a B. subtilis promoter. In: Molecular Cloning and Gene Regulation in Bacilli (Ganesan, A., Hoch, J. and Chang, S., Eds.), pp. 63-72. Academic Press, New York.
6. Shimotsu, H. and Henner, D.J. (1986) Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 43, 85-94.
7. Mogk, A., Hayward, R. and Schumann, W. (1996) Integrative vectors for constructing single-copy transciptional fusions between Bacillus subtilis promoters and various reporter genes encoding heat-stable enzymes. Gene 182, 33-36.
8. Yuan, G. and Wong, S. (1995) Isolation and characterization of Bacillus subtilis regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK. J. Bacteriol. 177, 6462-6468.
9. Saito, H., Shibata, T. and Ando, T. (1979) Mapping of genes determining nonpermissiveness and host-specific restriction to bacteriophages in Bacillus subtilis Marburg. Mol. Gen. Genet. 170, 117-122.
10. Schulz, A. and Schumann, W. (1996) hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat-shock genes. J. Bacteriol. 178, 1088-1093.
11. Hirata, H., Fukazawa, T., Negoro, S. and Okada, H. (1986) Structure of a β-galactosidase gene of Bacillus stearothermophilus. J. Bacteriol. 166, 722-727.
12. Harwood, C.R., Bell, D.E. and Winston, A.K. (1987) The effects of deletions in the leader sequence of cat-86, a chloramphenicol-resistance gene isolated from Bacillus pumilus. Gene 54, 267-273.
13. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
14. Hirata, H., Negoro, S. and Okada, H. (1985) High production of thermostable β-galactosidase of Bacillus stearothermophilus in Bacillus subtilis. Appl. Environ. Microbiol. 49, 1547-1549.
15. Homuth, G., Heinemann, M., Zuber, U. and Schumann, W. (1996) The genes lepA and hemN form a bicistronic operon in Bacillus subtilis. Microbiology 142, 1641-1649.
16. Hecker, M., Schumann, W. and Völker, U. (1996) Heat-shock and general stress response in Bacillus subtilis. Mol. Microbiol. 19, 417-428.
17. Schumann, W. (1996) The heat shock stimulon of Bacillus subtilis. Braz. J. Genet. 19, 387-398.
18. Homuth, G., Masuda, S., Mogk, A., Kobayashi, Y. and Schumann, W. (1997) The dnaK operon of Bacillus subtilis is heptacistronic. J. Bacteriol. (in press).
19. A-like promoter and the roles of the inverted repeat sequence (CIRCE). J. Bacteriol. 177, 5427-5433.
20. Harwood, C.R. and Bell, D.E. (1985) Structure and function of a chloramphenicol resistance gene cat-86 cloned in Bacillus subtilis. In: Gene Manipulation and Expression (Glass, R.E. and Spizek, J., Eds.), pp. 61-68. Croom Helm, London.
21. Harwood, C.R., Williams, D.M. and Lovett, P.S. (1983) Nucleotide sequence of a Bacillus pumilus gene specifying chloramphenicol acetyltransferase. Gene 24, 163-169.
22. Duvall, E.J., Williams, D.M., Lovett, P.S., Rudolph, C., Vasantha, N. and Guyer, M. (1983) Chloramphenicol-inducible gene expression in Bacillus subtilis. Gene 24, 171-177.
23. Williams, D.M., Schoner, R.G., Duvall, E.J., Preis, L.H. and Lovett, P.S. (1981) Expression of Escherichia coli trp genes and the mouse dihydrofolate reductase gene cloned in Bacillus subtilis. Gene 16, 199-206.