Quantitative determination of apoptotic death in cultured human pancreatic cancer cells by propidium iodide and digitonin

Cancer Letters

Volumn 142, Issue 2, 1999, Pages 129-137

  Zhang, Li ^a   Mizumoto, Kazuhiro ^a   Sato, Norihiro ^a   Ogawa, Takahiro ^a   Kusumoto, Masahiro ^a   Niiyama, Hideaki ^a   Tanaka, Masao ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Apoptosis; Pancreatic cancer; Propidium iodide

Indexed keywords

CYCLOHEXIMIDE; ETOPOSIDE; PROPIDIUM IODIDE; TRYPAN BLUE;

APOPTOSIS; ARTICLE; CELL DEATH; CELL GROWTH; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; IN VITRO STUDY; NECROSIS; PANCREAS CANCER; PRIORITY JOURNAL;

ANTINEOPLASTIC AGENTS, PHYTOGENIC; APOPTOSIS; CELL COUNT; CELL DIVISION; DIGITONIN; DRUG SCREENING ASSAYS, ANTITUMOR; ETOPOSIDE; HUMANS; MICROSCOPY, FLUORESCENCE; PANCREATIC NEOPLASMS; PROPIDIUM; TRYPAN BLUE; TUMOR CELLS, CULTURED;

EID: 0032974472     PISSN: 03043835     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3835(99)00107-X     Document Type: Article

References (29)

1. Wyline A.H., Kerr J.F.R., Currie A.R. Cell death: the significance of apoptosis. Int. Rev. Cytol. 68:1980;251-306.
2. Eguchi Y., Shimizu S., Tsujimoto Y. Intracellular ATP levels determine cell death fate by apoptosis or necrosis. Cancer Res. 57:1997;1835-1840.
3. Pantelidis P., Santilli G., Knight R.A. Sequential identification of apoptotic cells on individual cytospin preparations. J. Immunol. Methods. 214:1998;209-212.
4. Kerr J.F., Winterford C.M., Harmon B.V. Apoptosis. Its significance in cancer and cancer therapy. Cancer. 73:1994;2013-2026.
5. Paddenberg R., Wulf S., Weber A., Heimann P., Beck L.A., Mannherz H.G. Internucleosomal DNA fragmentation in cultured-cells under conditions reported to induce apoptosis may be caused by mycoplasma endonucleases. Eur. J. Cell Biol. 71:1996;105-119.
6. Huschtscha L.I., Bartier W.A., Ross C.E., Tattersall M.H. Characteristics of cancer cell death after exposure to cytotoxic drugs in vitro. Br. J. Cancer. 73:1996;54-60.
7. Eiseman J.L., Rogers F.A., Guo Y., Kauffman J., Sentz D.L., Klinger M.F., Callery P.S., Kyprianou N. Tumor-targeted apoptosis by a novel spermine analogue, 1,12-diaziridinyl-4,9-diazadodecane, results in therapeutic efficacy and enhanced radiosensitivity of human prostate cancer. Cancer Res. 58:1998;4864-4870.
8. Araki T., Taniwaki T., Becerra S.P., Chader G.J., Schwartz J.P. Pigment epithelium-derived factor (PEDF) differentially protects immature but not mature cerebella granule cells against apoptotic cell death. J. Neurosci. Res. 53:1998;7-15.
9. Farber J.L., Kyle M.E., Coleman J.B. Mechanisms of cell injury by activated oxygen species. Lab. Invest. 62:1990;670-679.
10. Perry S.W., Epstein L.G., Gelbard H.A. Simultaneous in situ detection of apoptosis and necrosis in monolayer cultures by TUNEL and Trypan Blue staining. Biotechniques. 22:1997;1102-1106.
11. Korystov Y.N., Shaposhnikova V.V., Levitman M.K., Kudryavtsev A.A., Kublik L.N., Narimanov A.A., Orlova O.E. The effect of inhibitors of arachidonic acid metabolism on proliferation and death of tumor cells. FEBS Lett. 431:1998;224-226.
12. GlascottJr P.A., Gilfor E., Serroni A., Farber J.L. Independent antioxidant action of vitamine E and C in cultured rat hepatocytes intoxicated with allyl alocohol. Biochem. Pharmacol. 52:1996;1245-1252.
13. Hoke G.D., Macia R.A., Meunier P.C., Bugelski P.J., Mirabelli C.K., Rush G.F., Matthews W.D. In vivo and in vitro cardiotoxicity of a gold-containing antineoplastic drug candidate in the rabbit. Toxicol. Appl. Pharmacol. 100:1989;293-306.
14. Reitman F.A., Shertzer H.G., Berger M.L. Toxicity of methylating agents in isolated hepatocytes. Biochem. Pharmacol. 37:1988;3183-3188.
15. Casini A., Giorli M., Hyland R.J., Serroni A., Gilfor D., Farber J.L. Mechanisms of cell injury in the killing of cultured hepatocytes by bromobenzene. J. Biol. Chem. 257:1982;6721-6728.
16. Miccadei S., Nakae D., Kyle M.E., Gilfor D., Farber J.L. Oxidative cell injury in the killing of cultured hepatocytes by allyl alcohol. Arch. Biochem. Biophys. 265:1988;302-310.
17. Wada M., Hosotani R., Lee J.U., Doi R., Koshiba T., Miyamoto Y., Tsuji S., Nakajima S., Okuyama A., Imamura M. An exogenous cdk inhibitor, butyrolactone-I, induces apoptosis with increased bax/bcl-2 ratio in p53-mutated pancreatic cancer cells. Anticancer Res. 18:1998;2559-2566.
18. Ikeguchi M., Tatebe S., Kaibara N., Ito H. Changes in levels of expression of p53 and the product of the bcl-2 in lines of gastric cancer cells during cisplatin-induced apoptosis. Eur. Surg. Res. 29:1997;396-402.
19. Medina V., Edminds B., Young G.P., James R., Appleton S., Zalewski P.D. Induction of caspase-3 protease activity and apoptosis by butyrate and trichostatin A (inhibitors of histone deacetylase): dependence on protein synthesis and synergy with a mitochondrial/cytochrome c-dependent pathway. Cancer Res. 57:1997;3697-3707.
20. Samejima K., Tone S., Kottke T.J., Enari M., Sakahira H., Cooke C.A., Durrieu F., Martins L.M., Nagata S., Kaufmann S.H., Earnshaw T.R. Transition from caspase-dependent to caspase-independent mechanisms at the onset of apoptotic execution. J. Cell Biol. 143:1998;225-239.
21. Kagawa S., Fujiwara T., Hizuta A., Yasuda T., Zhang W.W., Roth J.A., Tanaka N. expression overcomes P21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Oncogene. 15:1997;1903-1909.
22. Nieminen A.L., Gores G.J., Bond J.M., Imberti R., Herman B., Lemasters J.J. novel cytotoxicity screening assay using a multiwell fluorescence scanner. Toxicol. Appl. Pharm. 115:1992;147-155.
23. Hammerle T., Loffler M. Simultaneous analysis of mitochondrial activity and DNA content in Ehrlich ascites tumor cells by dual parameter flow cytometry. Histochemistry. 93:1989;207-212.
24. Khan S., Kayahara M., Loashi U., Mazarakis N.D., Sarraf C., Edwards A.D., Hughes M.N., Mehmet H. Differential induction of apoptosis in Swiss 3T3 cells by nitric oxide and the nitrosonium cation. J. Cell Sci. 110:1997;2315-2322.
25. Edwards Y.S., Sutherland L.M., Power J.H.T., Nicholas T.E., Murray A.W. Osmotic stress induces both secretion and apoptosis in rat alveolar type II cells. Am. J. Physiol. 275:1998;L670-L678.
26. Kebers F., Lewalle J.M., Desreux J., Munaut C., Devy L., Foidart J.M., Noel A. Induction of endothelial cell apoptosis by solid tumor cells. Exp. Cell Res. 240:1998;190-205.
27. Waldman T., Zhang Y.G., Dillehay L., Yu J., Kinzler K., Vogelstein B., Williams J. Cell-cycle arrest versus cell death in cancer therapy. Nature Med. 3:1997;1034-1037.
28. Wood D.E., Thomas A., Devi L.A., Berman Y., Beavis R.C., Reed J.C., Newcomb E.W. Bax cleavage is mediated by calpain during drug-induced apoptosis. Oncogene. 17:1998;1069-1078.
29. Celli A., Que F.G., Gores G.J., Larusso N.F. Glutathione depletion is associated with decreased Bcl-2 expression and increased apoptosis in cholangiocytes. Am. J. Physiol. 275:1998;G749-G757.