Simultaneous in situ detection of apoptosis and necrosis in monolayer cultures by TUNEL and Trypan Blue staining

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1102-1106

  Perry, S W ^a   Epstein, L G ^a   Gelbard, H A ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; DNA NUCLEOTIDYLEXOTRANSFERASE;

APOPTOSIS; ARTICLE; CELL DEATH; MEMBRANE PERMEABILITY; METHODOLOGY; MONOLAYER CULTURE; NECROSIS; STAINING;

EID: 0030927686     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226st01     Document Type: Article

References (5)

1. Adle-Biasette, H., Y. Levy, M. Colombel, F. Poron, S. Natchev, C. Keohane and F. Gray. 1995. Neuronal apoptosis in HIV infection in adults. Neuropathol. Appl. Neurobiol. 27:218-227.
2. Ankarcrona, M., J.M. Dypbukt, E. Bonfoco, B. Zhivotovsky, S. Orrenius, S.A. Lipton and P. Nicotera. 1995. Glutamate-induced neuronal death: a succession of necrosis or apoptosis depending on mitochondrial function. Neuron 15:961-973.
3. Bonfoco, E., D. Krainc, M. Ankarcrona, P. Nicotera and S.A. Lipton. 1995. Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc. Natl. Acad. Sci. USA 92:7162-7166.
4. DeRenzis, F.A. and A. Schechtman. 1973. Staining by Neutral Red and Trypan Blue in sequence for assaying vital and nonvital cultured cells. Stain Technol. 48:135-136.
5. Dive, C., C.D. Gregory, D.J. Phipps, D.L. Evans, A.E. Milner and A.H. Wyllie. 1992. Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochem. Biophys. Acta 1133:275-285.