Optimal prefixation of cells to demonstrate apoptosis by the TUNEL method

Acta Cytologica

Volumn 43, Issue 3, 1999, Pages 393-399

  Kiyozuka, Yasuhiko ^a   Akamatsu, Takako ^a   Singh, Yogendra ^a   Ichiyoshi, Hiroshi ^a   Senzaki, Hideto ^a   Tsubura, Airo ^a  

^a KANSAI MEDICAL UNIVERSITY   (Japan)

Author keywords

Apoptosis; Ovarian neoplasms; Tissue fixation; TUNEL

Indexed keywords

CISPLATIN; DNA FRAGMENT; DNA NUCLEOTIDYLEXOTRANSFERASE;

APOPTOSIS; ARTICLE; CANCER CELL; CANCER CHEMOTHERAPY; CELL ADHESION; CELL CULTURE; CELL STRUCTURE; COLORIMETRY; CYTODIAGNOSIS; DIAGNOSTIC ACCURACY; FEMALE; HUMAN; HUMAN CELL; OVARY CANCER; PRIORITY JOURNAL;

FALLOPIA;

EID: 0032959065     PISSN: 00015547     EISSN: 19382650     Source Type: Journal    
DOI: 10.1159/000331087     Document Type: Article

References (23)

1. Arends MJ, Morris RG, Wyllie AH: Apoptosis: The role of the endonuclease. Am J Pathol 1990;136:593-608
2. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 1979;18:5294-5299
3. Collins JA, Schandl CA, Young KK, Vesely J, Willingham MC: Major DNA fragmentation is a late event in apoptosis. J Histochem Cytochem 1997;45:923-934
4. Compton MM: A biochemical hallmark of apoptosis: Internucleosomal degradation of the genome. Cancer Metast Rev 1992;11:105-119
5. Cory AH, Owen TC, Barltrop JA, Cory JG: Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun 1991;3:207-212
6. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F: Features of apoptotic cells measured by flow cytometry. Cytometry 1992;13:795-808
7. Gavrieli Y, Sherman Y, Ben-Sasson SA: Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 1992;119:493-501
8. Gibb RK, Taylor DD, Wan T, O'Connor DM, Doering DL, Gercel-Taylor C: Apoptosis as a measure of chemosensitivity to cisplatin and taxol therapy in ovarian cancer cell lines. Gynecol Oncol 1997;65:13-22
9. Gold R, Schmied M, Giegerich G, Breitschopf H, Hartung HP, Toyka KV, Lassmann H: Differentiation between cellular apoptosis and necrosis by combined use of in situ tailing and nick translation techniques. Lab Invest 1994;71:219-225
10. Gorczyca W, Bruno S, Darzynkiewicz RJ, Gong J, Darzynkiewicz Z: DNA strand breaks occurring during apoptosis: Their early in situ detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors. Int J Oncol 1992;1: 639-648
11. Havrilesky LJ, Elbendary A, Hurteau JA, Whitaker RS, Rodriguez GC, Berchuck A: Chemotherapy-induced apoptosis in epithelial ovarian cancers. Obstet Gynecol 1995;85:1007-1010
12. Ichimura E, Fukuda T, Oyama T, Kashiwabara K, Sakurai S, Sano T, Nakajima T: Formalin fixation by boiling: Is it suitable for the TUNEL staining? Pathol Int 1995;45:971-972
13. Imai S, Kiyozuka Y, Maeda H, Noda T, Hosick HL: Establishment and characterization of a human ovarian serous cystadenocarcinoma cell line that produces the tumor markers CA-125 and tissue polypeptide antigen. Oncology 1990; 47:177-184
14. Kaye SB: Ovarian cancer, from the laboratory to the clinic: Challenges for the future. Ann Oncol 1996;7:9-13
15. Kerr JFR, Wyllie AH, Currie AR: Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 1972;26:239-257
16. Kerrigan D, Pommier Y, Kohn KW: Protein-linked DNA strand breaks produced by etoposide and teniposide in mouse L1210 and human VA-13 and HT-29 cell lines: Relationship to cytotoxicity. Natl Cancer Inst Monogr 1987;4: 117-121
17. Kiyozuka Y, Nishimura H, Nakashima A, Imamura K, Ota S, Yakushiji M, Imai S, Morimatsu M: In vitro cytotoxicity in ovarian cancer of low-dose continuous exposure to microtubule inhibitors (paclitaxel/taxotere) combined with cisplatin. Oncol Rep 1995;2:517-523
18. Negoescu A, Lorimier P, Labat-Moleur F, Drouet C, Robert C, Guillermet C, Brambilla C, Brambilla E: In situ apoptotic cell labeling by the TUNEL method: Improvement and evaluation on cell preparations. J Histochem Cytochem 1996;44: 959-968
19. Nemoto N, Nakamura H, Takashi K, lijima K, Furuse K, Osada H, Kinukawa N, Sakurai I: Fundamental study detecting apoptosis by the TUNEL (TdT-mediated d-UTP nick end-labeling) method in cytology preparation (in Japanese, abstract in English). J Jpn Soc Clin Cytol 1997;36:358-363
20. Perry SW, Epstein LG, Gelbard HA: Simultaneous in situ detection of apoptosis and necrosis in monolayer cultures by TUNEL and trypan blue staining. BioTechniques 1997;22: 1102-1106
21. Saccomanno G, Saunders RP, Ellis H, Archer VE, Wood BG, Beckler PA: Concentration of carcinoma or atypical cells in sputum. Acta Cytol 1963;7:305-310
22. Takeuchi T, Fujii A, Mimura Y, Hiraoka F, Hirata Y, Kamei M, Harada M, Takeuchi H, Murakami T: A study for TUNEL assay as a method of detecting apoptotic cells (in Japanese, abstract in English). J Jpn Soc Clin Cytol 1997;36:349-357
23. Tang C-S, Tang CMC, Lau Y-E, Kung ITM: Alcoholic carbowax prefixation and formal alcohol fixation: A new technique for urine cytology. Acta Cytol 1997;41:1183-1188