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Volumn 44, Issue 9, 1996, Pages 959-968

In situ apoptotic cell labeling by the TUNEL method: Improvement and evaluation on cell preparations

Author keywords

Chemiluminescence; Detergent; Double labeling; Enzymatic digestion; Microwaves; Preapoptosis; TUNEL

Indexed keywords

DNA NUCLEOTIDYLEXOTRANSFERASE;

EID: 0029835016     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.9.8773561     Document Type: Article
Times cited : (333)

References (57)
  • 1
    • 0025080576 scopus 로고
    • Activation of internucleosomal DNA cleavage in human CEM lymphocytes by glucocorticoid and novobiocin
    • Alnemri ES, Litwack G (1990) Activation of internucleosomal DNA cleavage in human CEM lymphocytes by glucocorticoid and novobiocin. J Biol Chem 265:17323-17333
    • (1990) J Biol Chem , vol.265 , pp. 17323-17333
    • Alnemri, E.S.1    Litwack, G.2
  • 2
    • 0027191429 scopus 로고
    • In situ end labeling detects DNA strand breaks in apoptosis and other physiological and pathological states
    • Ansari B, Coates BD, Greenstein BD, Hall PA (1993) In situ end labeling detects DNA strand breaks in apoptosis and other physiological and pathological states. J Pathol 170:1-8
    • (1993) J Pathol , vol.170 , pp. 1-8
    • Ansari, B.1    Coates, B.D.2    Greenstein, B.D.3    Hall, P.A.4
  • 4
    • 0027423114 scopus 로고
    • Estrogens inhibit and androgens enhance ovarian granulosa apoptosis
    • Billig H, Furuta I, Hsueh AJW (1993) Estrogens inhibit and androgens enhance ovarian granulosa apoptosis. Endocrinology 133:2204-2212
    • (1993) Endocrinology , vol.133 , pp. 2204-2212
    • Billig, H.1    Furuta, I.2    Hsueh, A.J.W.3
  • 5
    • 0027479655 scopus 로고
    • Dexamethasone-induced apoptosis involves cleavage of DNA to large fragments prior to internucleosomal fragmentation
    • Brown DG, Sun XM, Cohen GM (1992) Dexamethasone-induced apoptosis involves cleavage of DNA to large fragments prior to internucleosomal fragmentation. J Biol Chem 268:3037-3039
    • (1992) J Biol Chem , vol.268 , pp. 3037-3039
    • Brown, D.G.1    Sun, X.M.2    Cohen, G.M.3
  • 6
  • 7
    • 0025272539 scopus 로고
    • Determination of the length of the histological stages of apoptosis in normal liver and in altered hepatic foci of rats
    • Bursch W, Paffe S, Putz B, Barthel G, Schulte-Hermann R (1990b) Determination of the length of the histological stages of apoptosis in normal liver and in altered hepatic foci of rats. Carcinogenesis 11:847-853
    • (1990) Carcinogenesis , vol.11 , pp. 847-853
    • Bursch, W.1    Paffe, S.2    Putz, B.3    Barthel, G.4    Schulte-Hermann, R.5
  • 8
    • 0022411742 scopus 로고
    • Quantitative histological and histochemical studies on the occurrence and stages of controlled cell death (apoptosis) during regression of rat liver hyperplasia
    • Bursch W, Taper HS, Lauer B, Schulte-Hermann R (1985) Quantitative histological and histochemical studies on the occurrence and stages of controlled cell death (apoptosis) during regression of rat liver hyperplasia. Virchows Arch [Cell Pathol] 50:153-166
    • (1985) Virchows Arch [Cell Pathol] , vol.50 , pp. 153-166
    • Bursch, W.1    Taper, H.S.2    Lauer, B.3    Schulte-Hermann, R.4
  • 10
    • 0028963559 scopus 로고
    • An adherent cell model to study different stages of apoptosis
    • Desjardins LM, MacManus JP (1995) An adherent cell model to study different stages of apoptosis. Exp Cell Res 216:380-387
    • (1995) Exp Cell Res , vol.216 , pp. 380-387
    • Desjardins, L.M.1    MacManus, J.P.2
  • 11
    • 0026522112 scopus 로고
    • The origins of DNA breaks: A consequence of DNA damage, DNA repair, or apoptosis
    • Eastman A, Barry MA (1992) The origins of DNA breaks: a consequence of DNA damage, DNA repair, or apoptosis. Cancer Invest 10:229-240
    • (1992) Cancer Invest , vol.10 , pp. 229-240
    • Eastman, A.1    Barry, M.A.2
  • 12
    • 0026347249 scopus 로고
    • Significance and measurement of DNA double strand breaks in mammalian cells
    • Elia MC, DeLuca JG, Bradley MO (1991) Significance and measurement of DNA double strand breaks in mammalian cells. Pharmacol Ther 51:291-327
    • (1991) Pharmacol Ther , vol.51 , pp. 291-327
    • Elia, M.C.1    DeLuca, J.G.2    Bradley, M.O.3
  • 13
    • 0025816368 scopus 로고
    • Analysis of TNFa-induced DNA strand breaks at the single cell level
    • Fehsel K, Kolb-Bachofen V, Kolb H (1991) Analysis of TNFa-induced DNA strand breaks at the single cell level. Am J Pathol 139:251-254
    • (1991) Am J Pathol , vol.139 , pp. 251-254
    • Fehsel, K.1    Kolb-Bachofen, V.2    Kolb, H.3
  • 14
    • 0001206491 scopus 로고
    • Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia
    • Foley GE, Lazarus H, Farber S, Uzman BG, Boone BA, McCarthy RE (1965) Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18:522-529
    • (1965) Cancer , vol.18 , pp. 522-529
    • Foley, G.E.1    Lazarus, H.2    Farber, S.3    Uzman, B.G.4    Boone, B.A.5    McCarthy, R.E.6
  • 16
    • 0026648674 scopus 로고
    • Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
    • Gavrieli Y, Sherman Y, Ben-Sasson SA (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119:493-501
    • (1992) J Cell Biol , vol.119 , pp. 493-501
    • Gavrieli, Y.1    Sherman, Y.2    Ben-Sasson, S.A.3
  • 17
    • 0028136653 scopus 로고
    • Differentiation between cellular apoptosis and necrosis by combined use of in situ tailing and nick translation techniques
    • Gold R, Schmied M, Giegerich G, Breitschopf H, Hartung HP, Tokyka KV, Lassmann H (1994) Differentiation between cellular apoptosis and necrosis by combined use of in situ tailing and nick translation techniques. Lab Invest 71:219-225
    • (1994) Lab Invest , vol.71 , pp. 219-225
    • Gold, R.1    Schmied, M.2    Giegerich, G.3    Breitschopf, H.4    Hartung, H.P.5    Tokyka, K.V.6    Lassmann, H.7
  • 18
    • 0027163053 scopus 로고
    • Detection of DNA fragmentation in apoptosis: Application of in situ nick translation to cell culture systems and tissue sections
    • Gold R, Schmied M, Rothe G, Zischler H, Breitschopf H, Wekerle H, Lassmann H (1993) Detection of DNA fragmentation in apoptosis: application of in situ nick translation to cell culture systems and tissue sections. J Histochem Cytochem 41:1023-1030
    • (1993) J Histochem Cytochem , vol.41 , pp. 1023-1030
    • Gold, R.1    Schmied, M.2    Rothe, G.3    Zischler, H.4    Breitschopf, H.5    Wekerle, H.6    Lassmann, H.7
  • 19
    • 0027269557 scopus 로고
    • Detection of DNA strand breaks in individual apoptotic cells by the in situ TdT and nick translation assays
    • Gorczyca W, Gong J, Darzynkiewicz Z (1995) Detection of DNA strand breaks in individual apoptotic cells by the in situ TdT and nick translation assays. Cancer Res 53:1945-1951
    • (1995) Cancer Res , vol.53 , pp. 1945-1951
    • Gorczyca, W.1    Gong, J.2    Darzynkiewicz, Z.3
  • 20
    • 0027947764 scopus 로고
    • Detection of apoptosis-associated DNA strand breaks in fine-needle aspiration biopsies by in situ end labeling of fragmented DNA
    • Gorczyca W, Tuziak T, Kram A, Melamed MR, Darzynkiewicz Z (1993) Detection of apoptosis-associated DNA strand breaks in fine-needle aspiration biopsies by in situ end labeling of fragmented DNA. Cytometry 15:169-175
    • (1993) Cytometry , vol.15 , pp. 169-175
    • Gorczyca, W.1    Tuziak, T.2    Kram, A.3    Melamed, M.R.4    Darzynkiewicz, Z.5
  • 21
    • 0025898627 scopus 로고
    • Reduced apoptotic cell death in follicular lymphoma
    • Hollwood K, Macartney JC (1991) Reduced apoptotic cell death in follicular lymphoma. J Pathol 163:337-342
    • (1991) J Pathol , vol.163 , pp. 337-342
    • Hollwood, K.1    Macartney, J.C.2
  • 22
    • 0023017902 scopus 로고
    • DNA strand breaks in rat tissues as detected by in situ nick translation
    • Iseki S (1986) DNA strand breaks in rat tissues as detected by in situ nick translation. Exp Cell Res 167:311-326
    • (1986) Exp Cell Res , vol.167 , pp. 311-326
    • Iseki, S.1
  • 23
    • 0022068160 scopus 로고
    • Histochemical detection of DNA scissions in mammalian cells by in situ nick translation
    • Iseki S, Mori T (1985) Histochemical detection of DNA scissions in mammalian cells by in situ nick translation. Cell Biol Int Rep 9:471-477
    • (1985) Cell Biol Int Rep , vol.9 , pp. 471-477
    • Iseki, S.1    Mori, T.2
  • 24
    • 0002204915 scopus 로고
    • Definition and incidence of apoptosis: An historical perspective
    • Tomei LD, Cope FO, eds. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press
    • Kerr JFR, Harmon BV (1991) Definition and incidence of apoptosis: an historical perspective. In Tomei LD, Cope FO, eds. Apoptosis I: The Molecular Basis of Cell Death. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press, 5-29
    • (1991) Apoptosis I: The Molecular Basis of Cell Death , pp. 5-29
    • Kerr, J.F.R.1    Harmon, B.V.2
  • 25
    • 0015383455 scopus 로고
    • Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics
    • Kerr JFR, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239-257
    • (1972) Br J Cancer , vol.26 , pp. 239-257
    • Kerr, J.F.R.1    Wyllie, A.H.2    Currie, A.R.3
  • 26
    • 0023262077 scopus 로고
    • Protein-linked DNA strand breaks produced by etoposide and teniposide in mouse L1210 and human VA-13 and HT-29 cell lines: Relationship to cytotoxicity
    • Kerrigan D, Pommier Y, Kohn KW (1987) Protein-linked DNA strand breaks produced by etoposide and teniposide in mouse L1210 and human VA-13 and HT-29 cell lines: relationship to cytotoxicity. Natl Cancer Inst Monogr 4:117-121
    • (1987) Natl Cancer Inst Monogr , vol.4 , pp. 117-121
    • Kerrigan, D.1    Pommier, Y.2    Kohn, K.W.3
  • 28
    • 0028126775 scopus 로고
    • Distinction of apoptotic and necrotic cell death by in situ labelling of fragmented DNA
    • Kressel M, Groscurth P (1994) Distinction of apoptotic and necrotic cell death by in situ labelling of fragmented DNA. Cell Tissue Res 278:549-556
    • (1994) Cell Tissue Res , vol.278 , pp. 549-556
    • Kressel, M.1    Groscurth, P.2
  • 29
    • 0027430142 scopus 로고
    • Enhanced chemiluminescence: A highly sensitive detection system for in situ hybridization and immunohistochemistry
    • Lorimier P, Lamarcq L, Labat-Moleur F, Guillermet C, Bethier R, Stoebner P (1993) Enhanced chemiluminescence: a highly sensitive detection system for in situ hybridization and immunohistochemistry. J Histochem Cytochem 41:1591-1597
    • (1993) J Histochem Cytochem , vol.41 , pp. 1591-1597
    • Lorimier, P.1    Lamarcq, L.2    Labat-Moleur, F.3    Guillermet, C.4    Bethier, R.5    Stoebner, P.6
  • 30
  • 31
    • 0028891783 scopus 로고
    • Apoptosis, oncosis, and necrosis: An overview of cell death
    • Majno G, Joris I (1995) Apoptosis, oncosis, and necrosis: an overview of cell death. Am J Pathol 146:3-15
    • (1995) Am J Pathol , vol.146 , pp. 3-15
    • Majno, G.1    Joris, I.2
  • 32
    • 0026085951 scopus 로고
    • Effects of formaldehyde fixation on protein secondary structure: A calorimetric and infrared spetrocscopic investigation
    • Mason JT, O'Leary TJ (1991) Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spetrocscopic investigation. J Histochem Cytochem 39:225-229
    • (1991) J Histochem Cytochem , vol.39 , pp. 225-229
    • Mason, J.T.1    O'Leary, T.J.2
  • 33
    • 0029017072 scopus 로고
    • Ultrastructutal detection of DNA strand breaks in apoptotic neural cells by in situ end-labeling techniques
    • Migheli A, Atanasio A, Schiffer D (1995) Ultrastructutal detection of DNA strand breaks in apoptotic neural cells by in situ end-labeling techniques. J Pathol 176:27-35
    • (1995) J Pathol , vol.176 , pp. 27-35
    • Migheli, A.1    Atanasio, A.2    Schiffer, D.3
  • 34
    • 0027985477 scopus 로고
    • A study of apoptosis in normal and pathologic nervous tissue after in situ end labeling of DNA strand breaks
    • Migheli A, Cavalla P, Marino S, Schiffer D (1994) A study of apoptosis in normal and pathologic nervous tissue after in situ end labeling of DNA strand breaks. J Neuropathol Exp Neurol 53:606-616
    • (1994) J Neuropathol Exp Neurol , vol.53 , pp. 606-616
    • Migheli, A.1    Cavalla, P.2    Marino, S.3    Schiffer, D.4
  • 35
    • 0015452611 scopus 로고
    • Detection and measurement of single-strand breaks in nuclear DNA in fixed lens sections
    • Modak SP, Bollum FJ (1972) Detection and measurement of single-strand breaks in nuclear DNA in fixed lens sections. Exp Cell Res 75:307-313
    • (1972) Exp Cell Res , vol.75 , pp. 307-313
    • Modak, S.P.1    Bollum, F.J.2
  • 36
    • 0014861959 scopus 로고
    • Terminal lens cell differentiation
    • Modak SP, Bollum FJ (1970) Terminal lens cell differentiation. Exp Cell Res 62:421-432
    • (1970) Exp Cell Res , vol.62 , pp. 421-432
    • Modak, S.P.1    Bollum, F.J.2
  • 37
    • 0029039946 scopus 로고
    • The two in situ techniques do not differentiate between apoptosis and necrosis but rather reveal distinct patterns of DNA fragmentation in apoptosis
    • Mundle SD, Raza A (1995) The two in situ techniques do not differentiate between apoptosis and necrosis but rather reveal distinct patterns of DNA fragmentation in apoptosis. Lab Invest 72:611-613
    • (1995) Lab Invest , vol.72 , pp. 611-613
    • Mundle, S.D.1    Raza, A.2
  • 38
    • 0028055030 scopus 로고
    • F(ab) secondary antibodies: A general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence
    • Negoescu A, Labat-Moleur F, Lorimier P, Lamarcq L, Guillermet C, Chambaz EM, Brambilla E (1994) F(ab) secondary antibodies: a general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence. J Histochem Cytochem 42:433-437
    • (1994) J Histochem Cytochem , vol.42 , pp. 433-437
    • Negoescu, A.1    Labat-Moleur, F.2    Lorimier, P.3    Lamarcq, L.4    Guillermet, C.5    Chambaz, E.M.6    Brambilla, E.7
  • 39
    • 51249167530 scopus 로고
    • In situ end labeling and its applications to the study of endocrine disease: How can we study programmed cell death in surgical pathology materials?
    • Sasano H (1995) In situ end labeling and its applications to the study of endocrine disease: how can we study programmed cell death in surgical pathology materials? Endocrine Pathol 2:87-89
    • (1995) Endocrine Pathol , vol.2 , pp. 87-89
    • Sasano, H.1
  • 41
    • 0025829609 scopus 로고
    • Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections
    • Shi SR, Key ME, Kalra KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741-748
    • (1991) J Histochem Cytochem , vol.39 , pp. 741-748
    • Shi, S.R.1    Key, M.E.2    Kalra, K.L.3
  • 42
    • 0028792143 scopus 로고
    • Enhancement of mRNA in situ hybridization signal by microwave heating
    • Sibony M, Commo F, Callard P, Gasc JM (1995) Enhancement of mRNA in situ hybridization signal by microwave heating. Lab Invest 73:586-591
    • (1995) Lab Invest , vol.73 , pp. 586-591
    • Sibony, M.1    Commo, F.2    Callard, P.3    Gasc, J.M.4
  • 43
    • 0028960205 scopus 로고
    • Tumor type is a determinant of susceptibility to apoptosis
    • Staunton MJ, Gaffney EF (1995) Tumor type is a determinant of susceptibility to apoptosis. Am J Clin Pathol 103:300-307
    • (1995) Am J Clin Pathol , vol.103 , pp. 300-307
    • Staunton, M.J.1    Gaffney, E.F.2
  • 44
    • 0028985804 scopus 로고
    • Microwave irradiation of paraffin-embedded tissue sensitizes the TUNEL method for in situ detection of apoptotic cells
    • Sträter J, Günthert AR, Brüderlein S (1995) Microwave irradiation of paraffin-embedded tissue sensitizes the TUNEL method for in situ detection of apoptotic cells. Histochemistry 103:157-160
    • (1995) Histochemistry , vol.103 , pp. 157-160
    • Sträter, J.1    Günthert, A.R.2    Brüderlein, S.3
  • 45
    • 0028156903 scopus 로고
    • Separate metabolic pathways leading to DNA fragmentation and apoptotic chromatin condensation
    • Sun DY, Jiang S, Zheng LM, Ojcius DM, Young JDE (1994) Separate metabolic pathways leading to DNA fragmentation and apoptotic chromatin condensation. J Exp Med 179:559-568
    • (1994) J Exp Med , vol.179 , pp. 559-568
    • Sun, D.Y.1    Jiang, S.2    Zheng, L.M.3    Ojcius, D.M.4    Young, J.D.E.5
  • 46
    • 0025708015 scopus 로고
    • Dissassembly of chromatin into ∼50 kb units by detergent
    • Szabò G, Boldog F, Wikonkàl N (1990) Dissassembly of chromatin into ∼50 kb units by detergent. Biochem Biophys Res Commun 169:706-712
    • (1990) Biochem Biophys Res Commun , vol.169 , pp. 706-712
    • Szabò, G.1    Boldog, F.2    Wikonkàl, N.3
  • 47
    • 0028528079 scopus 로고
    • P53 immunolabeling in archival paraffin-embedded tissues: Optimal protocol based on microwave heating for eight antibodies on lung carcinomas
    • Tenaud C, Negoescu A, Labat-Moleur F, Legros Y, Soussi T, Brambilla E (1994) P53 immunolabeling in archival paraffin-embedded tissues: optimal protocol based on microwave heating for eight antibodies on lung carcinomas. Mod Pathol 7:853-859
    • (1994) Mod Pathol , vol.7 , pp. 853-859
    • Tenaud, C.1    Negoescu, A.2    Labat-Moleur, F.3    Legros, Y.4    Soussi, T.5    Brambilla, E.6
  • 49
    • 0001243106 scopus 로고
    • Apoptotic process in the radiation-induced death of lymphocytes
    • Tomei LD, Cope FO, eds. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press
    • Umanski SR (1991) Apoptotic process in the radiation-induced death of lymphocytes. In Tomei LD, Cope FO, eds. Apoptosis I: The Molecular Basis of Cell Death. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press, 193-208
    • (1991) Apoptosis I: The Molecular Basis of Cell Death , pp. 193-208
    • Umanski, S.R.1
  • 51
    • 0027138785 scopus 로고
    • Detection of the initial stages of DNA fragmentation in apoptosis
    • Walker RP, Kokileva L, Leblanc J, Sikorska M (1993) Detection of the initial stages of DNA fragmentation in apoptosis. Biotechniques 15:1032-1039
    • (1993) Biotechniques , vol.15 , pp. 1032-1039
    • Walker, R.P.1    Kokileva, L.2    Leblanc, J.3    Sikorska, M.4
  • 53
    • 0004050816 scopus 로고
    • London, San Diego, New York, Boston, Academic Press
    • Wolffe A (1995) Chromatin. 2nd ed. London, San Diego, New York, Boston, Academic Press
    • (1995) Chromatin. 2nd Ed.
    • Wolffe, A.1
  • 54
    • 0026504294 scopus 로고
    • Specific peptide-bond cleavage by microwave irradiation in weak acid solution
    • Wu CY, Chen ST, Chiou SH, Wang KT (1992) Specific peptide-bond cleavage by microwave irradiation in weak acid solution. J Prot Chem 11:45-50
    • (1992) J Prot Chem , vol.11 , pp. 45-50
    • Wu, C.Y.1    Chen, S.T.2    Chiou, S.H.3    Wang, K.T.4
  • 55
    • 0018830636 scopus 로고
    • Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
    • Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284:555-556
    • (1980) Nature , vol.284 , pp. 555-556
    • Wyllie, A.H.1
  • 57
    • 0021346318 scopus 로고
    • Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis
    • Wyllie AH, Morris RG, Smith AL, Dunlop D (1984) Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142:67-77
    • (1984) J Pathol , vol.142 , pp. 67-77
    • Wyllie, A.H.1    Morris, R.G.2    Smith, A.L.3    Dunlop, D.4


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