Blocking of endogenous avidin-binding activity in immunohistochemistry: The use of skim milk as an economical and effective substitute for commercial biotin solutions

Applied Immunohistochemistry

Volumn 7, Issue 1, 1999, Pages 63-65

  Miller, Rodney T ^a   Kubier, Patty ^a   Reynolds, Brenda ^a   Henry, Terry ^a   Turnbow, Holly ^a  

^a ProPath Laboratories Inc   (United States)

Author keywords

Antigen retrievat; Avidin; Biotin; Epitope retrieval; Immunocytochemistry; Immunohistochemistry; Milk

Indexed keywords

AVIDIN; BIOTIN;

ARTICLE; IMMUNOHISTOCHEMISTRY; MILK; PRIORITY JOURNAL; STAINING; TISSUE FIXATION;

EID: 0032958640     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: 10.1097/00022744-199903000-00013     Document Type: Article

References (6)

1. Wood GS, Warnke R. Suppression of endogenous avidin binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem 1981;29:1196-204.
2. Miller RT, Kubier P. Blocking of endogenous avidin-binding activity in immunohistochemistry: the use of egg whites. Appl Imnumohistochem 1997;5:63-6.
3. Rodriguez-Soto J, Warnke R, Rouse RV. Endogenous avidin-binding activity in paraffin-embedded tissue revealed after microwave treatment. Appl Immunohistochem 1997;5:59-62.
4. Savage D, Mattson G, Desai S, et al., eds. Avidin-biotin chemistry: a handbook, 2nd ed. Rockford, IL: Pierce Chemical, 1994:5.
5. Miller RT, Estran CE. Heat-induced epitope retrieval with a pressure cooker: suggestions for optimal use. Appl Immunohistochem 1995;3:190-3.
6. Butz WR, Clark KC, Miller RT. Improved manual immunohistochemistry employing orbital mixing of reagents during incubations. Appl Immunohistochem 1994;2:65-7.