In-vitro maturation of round spermatids using co-culture on Vero cells

Human Reproduction

Volumn 14, Issue 5, 1999, Pages 1287-1293

  Cremades, Nieves ^a   Bernabeu, Rafael ^a   Barros, Alberto ^b   Sousa, Mário ^b  

^a Instituto Bernabeu de Fertilidad y Ginecologia   (Spain)

^b NONE

Author keywords

Co culture; In vitro germ cell maturation; Nonobstructive azoospermia; Round spermatids; Vero cells

Indexed keywords

ARTICLE; AZOOSPERMIA; CASE REPORT; CELL CULTURE; FLAGELLUM; HUMAN; MALE; SPERMATID; SPERMATOCYTE; SPERMATOGENESIS; SPERMATOZOON; SPERMATOZOON MATURATION; TESTIS; VERO CELL;

EID: 0032951443     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/14.5.1287     Document Type: Article

References (53)

1. Amer, M., Soliman, E., El-Sadek, M. et al. (1997) Is complete spermiogenesis failure a good indication for spermatid conception? Lancet, 350, 116.
2. Angelopoulos, T., Krey, L., McCullough, A. et al. (1998) A simple and objective approach to identifying human round spermatids. Hum. Reprod., 12, 2208-2216.
3. Antinori, S., Versaci, C., Dani, G. et al. (1997a) Fertilization with human testicular spermatids: four successful pregnancies. Hum. Reprod., 12, 286-291.
4. Antinori, S., Versaci, C., Dani, G. et al. (1997b) Successful fertilization and pregnancy after injection of frozen-thawed round spermatids into human oocytes. Hum. Reprod., 12, 554-556.
5. Araki, Y., Motoyama, M., Yoshida, A. et al. (1997) Intracytoplasmic injection with late spermatids: a successful procedure in achieving childbirth for couples in which the male partner suffers from azoospermia due to deficient spermatogenesis. Fertil. Steril., 67, 559-561.
6. Aslam, I. and Fishel, S. (1998) Short-term in-vitro culture and cryopreservation of spermatogenetic cells used for human in-vitro conception. Hum. Reprod., 13, 634-638.
7. Aslam, I., Fishel, S., Green, S. et al. (1998a) Can we justify spermatid micro-injection for severe male factor infertility? Hum. Reprod. Update, 4, 213-222.
8. Aslam, I., Robins, A., Dowell, K. et al. (1998b) Isolation, purification and assessment of viability of spermatogenic cells from testicular biopsies of azoospermic men. Hum. Reprod., 13, 639-645.
9. Balet, R., Egbase, P. and Grudzinskas, J.G. (1998) TESE and the distribution of spermatogenesis in the testicles of azoospermic men. Hum. Reprod., 13, 2034.
10. Barak, Y., Kogosowski, A., Goldman, S. et al. (1998) Pregnancy and birth after transfer of embryos that developed from single-nucleated zygotes obtained by injection of round spermatids into oocytes. Fertil. Steril., 70, 67-70.
11. Barros, A., Bernabeu, R., Takahashi, K. et al. (1998a) Intracytoplasmic injection of ejaculate and testicle spermatids: report on 35 cycles. Hum. Reprod., 13 (abstract bk 1), 154-155.
12. Barros, A., Takahashi, K., Bernabeu, R. et al. (1998b) Spermatid intracytoplasmic injection: report on 56 cycles. Fertil. Steril., (suppl.) S441.
13. Bernabeu, R., Cremades, N., Takahashi, K. et al. (1998) Successful pregnancy after spermatid injection. Hum. Reprod., 13, 1898-1900.
14. Chen, S-U., Ho, H-N., Chen, H-F. et al. (1996) Fertilization and embryo cleavage after intracytoplasmic spermatid injection in an obstructive azoospermic patient with defective spermiogenesis. Fertil. Steril., 66, 157-160.
15. Devroey, P. (1998) Are spermatid injections of any clinical value? Testicular sperm extraction and intracytoplasmic sperm injection. Hum. Reprod., 13, 2045-2046.
16. Edwards, R.G., Tarin, J.J., Dean, N. et al. (1994) Are spermatid injections into human oocytes now mandatory? Hum. Reprod., 9, 2217-2219.
17. Fishel, S., Green, S., Bishop, M. et al. (1995) Pregnancy after intracytoplasmic injection of spermatid. Lancet, 345, 1641-1642.
18. Fishel, S., Aslam, I. and Tesarik, J. (1996) Spermatid conception: a stage too early, or a time too soon? Hum. Reprod., 11, 1371-1375.
19. Fishel, S., Green, S., Hunter, A. et al. (1997) Human fertilization with round and elongated spermatids. Hum. Reprod., 12, 336-340.
20. Hannay, T. (1995) New Japanese IVF method finally made available in Japan. Nature Med., 1, 289-290.
21. Heller, C.G. and Clermont, Y. (1963) Spermatogenesis in man: An estimate of its duration. Science, 140, 184.
22. Hue, D., Staub, C., Perrard-Sapori, M-H. et al. (1998) Meiotic differentiation of germinal cells in three-week cultures of whole population from rat seminiferous tubules. Biol. Reprod., 59, 379-387.
23. Kaharaman, S., Polat, G., Samli, M. et al. (1998) Multiple pregnancies obtained by testicular spermatid injection in combination with intracytoplasmic sperm injection. Hum. Reprod., 13, 104-110.
24. Mansour, R.T., Aboulghar, M.A., Serour, G.I. et al. (1996) Pregnancy and delivery after intracytoplasmic injection of spermatids into human oocytes. Middle East Fertil. Soc. J., 1, 223-225.
25. Mendoza, C. and Tesarik, J. (1996) The occurrence and identification of round spermatids in the ejaculate of men with nonobstructive azoospermia. Fertil. Steril., 66, 826-829.
26. Mendoza, C., Benkhalifa, M., Cohen-Bacrie, P. et al. (1996) Combined use of proacrosin immunocytochemistry and autosomal DNA in situ hybridisation for evaluation of human ejaculated germ cells. Zygote, 4, 279-283.
27. Ménézo, Y.J.R., Guerin, J-F. and Czyba, J-C. (1990) Improvement of human early embryo development in-vitro by co-culture on monolayers of Vero cells. Biol. Reprod., 42, 301-306.
28. Ménézo, Y., Hazout, A., Dumont, M. et al. (1992) Coculture of embryos on Vero cells and transfer of blastocysts in humans. Hum. Reprod., 7 (suppl.), 101-106.
29. Nakamura, M., Romrell, L.J. and Hall, P.F. (1978) The effects of temperature and glucose on protein biosynthesis by immature (round) spermatids from rat testes. J. Cell Biol., 79, 1-9.
30. Ouhibi, N., Ménézo, Y., Benet, G. et al. (1989) Culture of epithelial cells derived from the oviduct of different species. Hum. Reprod., 4, 229-235.
31. Schillaci, R., Ciriminna, R. and Cefalù, E. (1994) Vero cell effect on in-vitro human blastocyst development: preliminary results. Hum. Reprod., 9, 1131-1135.
32. Silber, S.J. (1998) TESE and the distribution of spermatogenesis in the testicles of azoospermic men. Hum. Reprod., 13, 2035.
33. Silber, S.J. and Johnson, L. (1998) Are spermatid injections of any clinical value? ROSNI and ROSI revisited. Hum. Reprod., 13, 509-515.
34. Silber, S., Nagy, Z., Devroey, P. et al. (1997) Distribution of spermatogenesis in the testicles of azoospermic men: the presence or absence of spermatids in the testes of men with germinal failure. Hum. Reprod., 12, 2422-2428.
35. Sofikitis, N., Miyagawa, I., Yamamoto, Y. et al. (1998a) Micro- and macro-consequences of ooplasmic injections of early haploid male gametes. Hum. Reprod. Update, 4, 197-212.
36. Sofikitis, N.V., Yamamoto, Y., Miyagawa, I. et al. (1998b) Ooplasmic injection of elongating spermatids for the treatment of non-obstructive azoospermia. Hum. Reprod., 13, 709-714.
37. Sousa, M., Barros, A. and Tesarik, J. (1998) Current problems with spermatid conception. Hum. Reprod., 13, 255-258.
38. Sousa, M., Barros, A., Takahashi, K. et al. (1999) Clinical efficacy of spermatid conception: analysis using a new spermatid classification scheme. Hum. Reprod., 14, 1279-1286.
39. Sousa, M., Mendoza, C., Barros, A. et al. (1996) Calcium responses of human oocytes after intracytoplasmic injection of leukocytes, spermatocytes and round spermatids. Mol. Hum. Reprod., 2, 853-857.
40. Tesarik, J. (1996) Fertilization of oocytes by injecting spermatozoa, spermatids and spermatocytes. Rev. Reprod., 1, 149-152.
41. Tesarik, J. (1997a) Sperm or spermatid conception? Fertil. Steril., 68, 214-216.
42. Tesarik, J. (1997b) La fécondation humaine sans spermatozoides. La Recherche, 295, 78-83.
43. Tesarik, J. and Mendoza, C. (1996a) Spermatid injection into human oocytes. I. Laboratory techniques and special features of zygote development. Hum. Reprod., 11, 772-779.
44. Tesarik, J. and Mendoza, C. (1996b) Genomic imprinting abnormalities: a new potential risk of assisted reproduction. Mol. Hum. Reprod., 2, 295-298.
45. Tesarik, J., Mendoza, C. and Testart, J. (1995) Viable embryos from injection of round spermatids into oocytes. N. Engl. J. Med., 333, 525.
46. Tesarik, J., Rolet, F., Brami, C. et al. (1996) Spermatid injection into human oocytes. II. Clinical application in the treatment of infertility due to non-obstructive azoospermia. Hum. Reprod., 11, 780-783.
47. Tesarik, J., Greco, E. and Mendoza, C. (1998a) ROSI, instructions for use: 1997 update. Hum. Reprod., 13, 519-523.
48. Tesarik, J., Sousa, M., Greco, E. et al. (1998b) Spermatids as gametes: indications and limitations. Hum. Reprod., 13 (Suppl. 3), 89-111.
49. Vanderzwalmen, P., Lejeune, B., Nijs, M. et al. (1995) Fertilization of an oocyte micro-inseminated with a spermatid in an in-vitro fertilization programme. Hum. Reprod., 10, 502-503.
50. Vanderzwalmen, P., Zech, H., Birkenfeld, A. et al. (1997) Intracytoplasmic injection of spermatids retrieved from testicular tissue: influence of testicular pathology, type of selected spermatids and oocyte activation. Hum. Reprod., 12, 1203-1213.
51. Vanderzwalmen, P., Nijs, M., Schoysman, R. et al. (1998) The problems of spermatid microinjection in the human: the need for an accurate morphological approach and selective methods for viable and normal cells. Hum. Reprod., 13, 515-519.
52. Verheyen, G., Crabbe, E., Joris, H. et al. (1998) Simple and reliable identification of the human round spermatid by inverted phase contrast microscopy: does the real target group exist? Hum. Reprod., 13, 1570-1577.
53. Yamanaka, K., Sofikitis, N.V., Miyagawa, I. et al. (1997) Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia. J. Ass. Reprod. Genetics, 14, 55-62.