Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception

Human Reproduction

Volumn 13, Issue 3, 1998, Pages 634-638

  Aslam, Irfan ^a   Fishel, Simon ^a  

^a Park Hospital   (United Kingdom)

Author keywords

Cryopreservation; In vitro culture; Male infertility; Microinjection; Spermatids

Indexed keywords

ARTICLE; AZOOSPERMIA; CELL CULTURE; CELL MATURATION; CELL SHAPE; CELL STRUCTURE; CELL VIABILITY; COCULTURE; CONTROLLED STUDY; CRYOPRESERVATION; FERTILIZATION IN VITRO; FLAGELLUM; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; SPERMATID; SPERMATOCYTE; TESTIS BIOPSY;

EID: 0031942057     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/13.3.634     Document Type: Article

References (33)

1. Aslam, I. and Fishel, S. (1996) The use of spermatids for human conception. In Hansson, V., Levy, P.O. and Tasken, K. (eds), Signal Transduction in Testicular Cells. Springer-Verlag, Berlin, Germany, pp. 271-286.
2. Aslam, I., Robins, R.A., Dowell, K. et al. (1997) Isolation, purification and assessment of viability of spermatogenic cells from testicular biopsies of azoospermic men. Hum. Reprod., 13, 639-645.
3. Bellve, R.A. (1993) Purification, culture and fractionation of spermatogenic cells. Methods Enzymol., 225, 84-113.
4. Craft, I. and Tsirigotis, M. (1995) Simplified recovery, preparation and cryopreservation of testicular spermatozoa. Hum. Reprod., 10, 1623-1627.
5. Fishel, S., Green, S., Bishop, M. et al. (1995) Pregnancy after intracytoplasmic injection of spermatid. Lancet, 345, 1641-1642.
6. Fishel, S. Aslam, I. and Tesarik, J. (1996) Spermatid conception: a stage too early, or a time too soon? Hum. Reprod., 11, 1371-1375.
7. Fishel, S., Green, S., Hunter, A. et al. (1997) Human conception with round and elongated spermatids. Hum. Reprod., 12, 336-340.
8. Gerton, G.L. and Millette, C.F. (1984) Generation of flagella by cultured mouse spermatids. J. Cell Biol., 98, 619-628.
9. Gerton, G.L. and Millette, C.F. (1986) Stage-specific synthesis and fucosylation of plasma membrane proteins by mouse pachytene spermatocytes and round spermatids in culture. Biol. Reprod., 35, 1025-1035.
10. Grimes, S.R. (1986) Nuclear proteins in spermatogenesis. Compar. Biochem. Physiol. B, 83, 495-500.
11. Grimes, S.R., Platz, R.D., Meistrich, M.L. et al. (1977) Nuclear protein transitions in rat testis spermatids. Exp. Cell. Res., 110, 31-39.
12. Kimura, Y. and Yanagimachi, R. (1995) Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring. Development, 121, 2397-2405.
13. Lanneau, M. and Loir, M. (1982) An electrophoretic investigation of mammalian spermatid specific nuclear proteins. J. Reprod. Fertil., 65, 163-170.
14. Meistrich, M., Brock, W.A., Grimes, S.R. et al. (1978) Nuclear protein transitions during spermatogenesis. Fed. Proc., 37, 2522-2525.
15. Millette, C. and Moulding, C.T. (1981) Cell surface marker proteins during mouse spermatogenesis: two-dimensional electrophoretic analysis. J. Cell Sci., 48, 367-382.
16. Montag, M., Tok, V., Liow, S. et al. (1992) In vitro decondensation of mammalian sperm and subsequent formation of pronuclei-like structures for micromanipulation. Mol. Reprod. Dev., 33, 338-346.
17. Nakamura, M., Romrell, L.J. and Hall, P.F. (1978) The effects of temperature and glucose on protein biosynthesis by immature (round) spermatids from rat testes. J. Cell Biol., 79, 1-9.
18. Nakamura, M., Okinaga, S. and Arai, K. (1984) Metabolism of round spermatids: evidence that lactate is the preferred substrate. Am. J. Physiol., 247, E234-E242.
19. Nonchev, S. and Tsanev, R. (1990) Protamine-histone replacement and DNA replication in the male mouse pronucleus. Mol. Reprod. Dev., 25, 72-76.
20. O'Brien, D.A. (1987) Stage-specific protein synthesis by isolated spermatogenic cells throughout meiosis and early spermiogenesis in the mouse. Biol. Reprod., 37, 147-157.
21. O'Brien D.A. (1993) Isolation, separation, and short-term culture of spermatogenic cells. In Chapin, R.E. and Heindel, J.J. (eds), Methods in Toxicology. Academic Press, New York, NY, USA, Vol. 3A, pp. 246-264.
22. Ogura, A., Matsuda, J., Asano, T. et al. (1996) Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring. J. Assist. Reprod. Genet., 13, 431-434.
23. Parvinen, M., Wright, W.W., Phillips, D.M. et al. (1983) Spermatogenesis in vitro: completion of meiosis and early spermiogenesis. Endocrinology, 112, 1150-1152.
24. Perreault, S.D., Naish, S.J. and Zirkin, B.R. (1987) The timing of hamster sperm nuclear decondensation and male pronucleus formation is related to sperm nuclear disulphide bond content. Biol. Reprod., 36, 239-244.
25. Phillips, H.J. (1973) Evaluation of culture dynamics. Dye exclusion test for cell viability. In Kruse, P.K. and Patterson, M.K. (eds). Tissue Culture Methods and Applications. Academic Press, New York, NY, USA, pp. 406-408.
26. Poccia, D. (1986) Remodelling of nucleoproteins during gametogenesis, fertilisation, and early development. Int. Rev. Cytol., 105, 1-65.
27. Salzbrunn, A., Benson, D.M., Holstein, A.F. et al. (1996) A new concept for the extraction of testicular spermatozoa as a tool for assisted fertilisation (ICSI). Hum. Reprod., 11, 752-755.
28. Steinberger, A. (1975) In vitro techniques for the study of spermatogenesis. Methods Enzymol., 39, 283.
29. Steinberger, A. and Steinberger, E. (1966) In vitro culture of rat testicular cells. Exp. Cell. Res., 44, 443-452.
30. Talbot, P. and Chacon, R.S. (1981) A triple-stain technique for evaluating normal acrosome reaction of human sperm. J. Exp. Zool., 215, 201-208.
31. Tesarik, J. (1996) Fertilisation of oocytes by injecting spermatozoa, spermatids and spermatocytes. Rev. Reprod., 1, 149-152.
32. Tesarik, J. and Mendoza, C. (1996) Spermatid injection into human oocytes. I. Laboratory techniques and special features of zygote development. Hum. Reprod., 11, 772-779.
33. Tesarik, J., Mendoza, C. and Testart, J. (1995) Viable embryos from injection of round spermatids into oocytes. N. Engl. J. Med., 333, 525.