Functional reconstitution of bacterially expressed human potassium channels in proteoliposomes: Membrane potential measurements with JC-1 to assay ion channel activity

Biochimica et Biophysica Acta - Biomembranes

Volumn 1416, Issue 1-2, 1999, Pages 92-100

  Chanda, Baron ^a   Mathew, M K ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Fluorescence assay; JC 1; Membrane potential; Potassium channel; Reconstitution

Indexed keywords

4 AMINOPYRIDINE; CYANINE DYE; POTASSIUM CHANNEL;

ARTICLE; CELL MEMBRANE POTENTIAL; ESCHERICHIA COLI; HUMAN; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEOLIPOSOME; SPECTROPHOTOMETRY;

4-AMINOPYRIDINE; BACTERIA; BENZIMIDAZOLES; CARBOCYANINES; CLONING, MOLECULAR; FLUORESCENT DYES; HUMANS; LIPOSOMES; MEMBRANE POTENTIALS; POTASSIUM CHANNEL BLOCKERS; POTASSIUM CHANNELS; PROTEOLIPIDS;

EID: 0032951123     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0005-2736(98)00217-X     Document Type: Article

References (24)

1. T. Sun, A.A. Naini, C. Miller, Biochemistry 33 (1994) 9992-9999.
2. R.H. Spencer, Y. Sukolov, H. Li, B. Tanenaka, A.J. Milici, J. Aiyar, A. Nguyen, H. Park, B.K. Jap, J.E. Hall, G.A. Gutman, K.G. Chandy, J. Biol. Chem. 272 (1997) 2389-2395.
3. D.A. Doyle, J.M. Cabral, R.A. Pfuetzner, A. Kuo, J.M. Gulbis, S.L. Cohen, B.T. Chait, R. MacKinnon, Science 280 (1998) 69-77.
4. B. Hille, Ionic Channels of Excitable Membranes, 2nd edn., Sinauer Associates Inc., Massachusetts, 1992.
5. L.M. Mannuzzu, M.M. Moronne, E.Y. Isacoff, Science 271 (1996) 213-216.
6. M. Ramaswami, M. Gautam, A. Kamb, B. Rudy, M.A. Tanouye, M.K. Mathew, Mol. Cell. Neurosci. 1 (1990) 214-223.
7. S.T. Smiley, M. Reers, C.M. Harthshorn, M. Lin, A. Chen, T.W. Smith Jr., G.D.S. and L.B. Chen, Proc. Natl. Acad. Sci. USA 88 (1991) 3671-3675.
8. S. Seth, P. Balaram, M.K. Mathew, Biochim. Biophys. Acta 1328 (1997) 177-184.
9. R.R.C. New, Liposomes: a Practical Approach Series, IRL Press, Oxford, 1990.
10. P.J. Sims, A.S. Waggoner, C.H. Wang, J.F. Hoffman, Biochemistry 13 (1974) 3315-3330.
11. L. Santacruz-Toloza, E. Perozo, D.M. Papazian, Biochemistry 33 (1994) 1295-1299.
12. Mees, in: The Theory of Photographic Process, Macmillan Press, New York, 1965, pp. 430-499.
13. D.M. Sturmer, D.W. Heseltine, in: T.H. James (Ed.), The Theory of Photographic Process, Macmillan, New York, 1977, pp. 194-235.
14. M. Reers, S.T. Smiley, C. Mottola-Hartshorn, A. Chen, M. Lin, L.B. Chen, Methods Enzymol. 260 (1995) 406-417.
15. M. Reers, T.W. Smith, L.B. Chen, Biochemistry 30 (1991) 4480-4486.
16. J.R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, New York, 1983.
17. H. Rehm, S. Pelzer, C. Cochet, E. Chambaz, B.L. Tempel, W. Trautwein, D. Pelzer, M. Lazdunski, Biochemistry 28 (1989) 6455-6460.
18. G. Fiermonte, J.E. Walker, F. Palmieri, Biochem. J. 294 (1993) 293-299.
19. L. Santacruz-Toloza, Y. Huang, S.A. John, D.M. Papazian, Biochemistry 33 (1994) 5607-5613.
20. M. Montal, R. Anholt, P. Labarca, in: C. Miller (Ed.), Ion Channel Reconstitution, Plenum, New York, 1985.
21. R.L. Huganir, E. Racker, J. Biol. Chem. 257 (1982) 9372-9378.
22. L. Heginbotham, E. Odessey, C. Miller, Biochemistry 36 (1997) 10335-10342.
23. D.J. Woodbury, C. Miller, Biophys. J. 58 (1990) 833-839.
24. C. Miller, Annu. Rev. Physiol. 46 (1984) 549-558.