Ku-dependent nonhomologous DNA end joining in Xenopus egg extracts

Molecular and Cellular Biology

Volumn 19, Issue 4, 1999, Pages 2585-2593

  Labhart, Paul ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DEPENDENT PROTEIN KINASE;

ANIMAL CELL; ARTICLE; DNA DNA HYBRIDIZATION; DNA RECOMBINATION; DNA REPAIR; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; XENOPUS;

ANIMALIA; EUKARYOTA; MAMMALIA;

EID: 0032934980     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.19.4.2585     Document Type: Article

References (41)

1. Abu-Elheiga, L., and M. Yaneva. 1992. Antigenic determinants of the 70-kDa subunit of the Ku autoantigen. Clin. Immunol. Immunopathol. 64:145-152.
2. Anderson, C. W., and T. H. Carter. 1996. The DNA-activated protein kinase: DNA-PK. Curr. Top. Microbiol. Immunol. 217:91-111.
3. Anderson, C. W., and S. P. Lees-Miller. 1992. The nuclear serine/threonine protein kinase DNA-PK. Crit. Rev. Eukaryotic Gene Expr. 2:283-314.
4. Blunt, T., N. J. Finnie, G. E. Taccioli, G. C. M. Smith, J. Demengeot, T. M. Gottlieb, R. Mizuta, A. J. Varghese, F. W. Alt, P. A. Jeggo, and S. P. Jackson. 1995. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell 80:813-823.
5. Boulton, S. J., and S. P. Jackson. 1996. Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance. Nucleic Acids Res. 24:4639-4648.
6. Carter, T., I. Vancurova, I. Sun, W. Lou, and S. DeLeon. 1990. A DNA-activated protein kinase from HeLa cell nuclei. Mol. Cell. Biol. 10:6460-6471.
7. Chu, G. 1997. Double strand break repair. J. Biol. Chem. 272:24097-24100.
8. Cortes, P., F. Weis-Garcia, Z. Misulovin, A. Nussenzweig, J.-S. Lai, G. Li, M. C. Nussenzweig, and D. Baltimore. 1996. In vitro V(D)J recombination: signal joint formation. Proc. Natl. Acad. Sci. USA 93:14008-14013.
9. Daza, P., S. Reichenberger, B. Göttlich, M. Hagmann, E. Feldmann, and P. Pfeiffer. 1996. Mechanisms of nonhomologous DNA end joining in frogs, mice and men. Biol. Chem. Hoppe-Seyler 377:775-786.
10. Derbyshire, M. K., L. H. Epstein, C. S. H. Young, P. L. Munz, and R. Fishel. 1994. Nonhomologous recombination in human cells. Mol. Cell. Biol. 14: 156-169.
11. Dynan, W. S., and S. Yoo. 1998. Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids. Nucleic Acids Res. 26:1551-1559.
12. Goedecke, W., W. Vielmetter, and P. Pfeiffer. 1992. Activation of a system for the joining of nonhomologous DNA ends during Xenopus egg maturation. Mol. Cell. Biol. 12:811-816.
13. Gottlieb, T. M., and S. P. Jackson. 1993. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell 72:131-142.
14. Gravel, S., M. Larrivée, P. Labrecque, and R. J. Wellinger. 1998. Yeast Ku as a regulator of chromosomal DNA end structure. Science 280:741-744.
15. Gu, X.-Y., R. A. O. Bennett, and L. F. Povirk. 1996. End-joining of free radical-mediated DNA double-strand breaks in vitro is blocked by the kinase inhibitor wortmannin at a step preceding removal of damaged 3′ termini. J. Biol. Chem. 271:19660-19663.
16. Gu, X.-Y., M. A. Weinfeld, and L. F. Povirk. 1998. Implication of DNA-dependent protein kinase in an early, essential, local phosphorylation event during end-joining of DNA double-strand breaks in vitro. Biochemistry 37: 9827-9835.
17. Gurdon, J. B., and M. P. Wickens. 1983. The use of Xenopus oocytes for the expression of cloned genes. Methods Enzymol. 101:370-386.
18. Hagmann, M., K. Adlkofer, P. Pfeiffer, K. Bruggmann, O. Georgiev, D. Rungger, and W. Schaffner. 1996. Dramatic changes in the ratio of homologous recombination to nonhomologous DNA-end joining in oocytes and early embryos of Xenopus laevis. Biol. Chem. Hoppe-Seyler 377:239-250.
19. Hartley, K. O., D. Gell, G. C. M. Smith, H. Zhang, N. Divecha, M. A. Connelly, A. Admon, S. P. Lees-Miller, C. W. Anderson, and S. P. Jackson. 1995. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell 82:849-856.
20. Jackson, S. P., and P. A. Jeggo. 1995. DNA double-strand break repair and V(D)J recombination: involvement of DNA-PK. Trends Biol. Sci. 20:412-415.
21. Kirchgessner, C. U., C. K. Patil, J. W. Evans, C. A. Cuomo, L. M. Fried, T. Carter, M. A. Oettinger, and J. M. Brown. 1995. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267:1178-1183.
22. Knuth, M. W., S. I. Gunderson, N. E. Thompson, L. A. Strasheim, and R. R. Burgess. 1990. Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J. Biol. Chem. 265:17911-17920.
23. Labhart, P. 1995. The Xenopus 9-bp ribosomal terminator (T3 box) is a pause signal for the RNA polymerase I elongation complex. Nucleic Acids Res. 23:2252-2258.
24. Labhart, P. 1997. mRNA encoding the catalytic subunit of DNA-dependent protein kinase is widely expressed in Xenopus cells. Gene 203:235-240.
25. Labhart, P. Unpublished observations.
26. Lehman, C. W., M. Clemens, D. K. Worthylake, J. K. Trautman, and D. Carroll. 1993. Homologous and illegitimate recombination in developing Xenopus oocytes and eggs. Mol. Cell. Biol. 13:6897-6906.
27. Lieber, M. R., J. E. Hesse, S. Lewis, G. C. Bosma, N. Rosenberg, K. Mizuuchi, M. J. Bosma, and M. Gellert. 1988. The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination. Cell 55:7-16.
28. Mimori, T., and J. A. Hardin. 1986. Mechanism of interaction between Ku protein and DNA. J. Biol. Chem. 261:10375-10379.
29. Nicolas, A. L., and C. S. H. Young. 1994. Characterization of DNA end joining in a mammalian cell nuclear extract: junction formation is accompanied by nucleotide loss, which is limited and uniform but not site specific. Mol. Cell. Biol. 14:170-180.
30. Pang, D., S. Yoo, W. S. Dynan, M. Jung, and A. Dritschilo. 1997. Ku proteins join DNA fragments as shown by atomic force microscopy. Cancer Res. 57:1412-1415.
31. Pfeiffer, P., S. Thode, J. Hancke, and W. Vielmetter. 1994. Mechanisms of overlap formation in nonhomologous DNA end joining. Mol. Cell. Biol. 14:888-895.
32. Pfeiffer, P., and W. Vielmetter. 1988. Joining of nonhomologous DNA double strand breaks in vitro. Nucleic Acids Res. 16:907-924.
33. Reeves, W. H. 1985. Use of monoclonal antibodies for the characterization of novel DNA-binding proteins recognized by human autoimmune sera. J. Exp. Med. 161:18-39.
34. Reeves, W. H. 1992. Antibodies to the p70/p80 (Ku) antigens in systemic lupus crythematosus. Rheum. Dis. Clin. N. Am. 18:391-414.
35. Reeves, W. H., A. Pierani, C. H. Chou, T. Ng, C. Nicastri, R. G. Roeder, and Z. M. Sthoeger. 1991. Epitopes of the p70 and p80 (Ku) lupus autoantigens. J. Immunol. 146:2678-2686.
36. Schaal, H., P. Pfeiffer, M. Klein, P. Gehrmann, and A. Scheid. 1993. Use of DNA end joining activity of a Xenopus laevis egg extract for construction of deletions and expression vectors for HIV-1 Tat and Rev proteins. Gene 124:275-280.
37. Smider, V., W. K. Rathmell, M. R. Lieber, and G. Chu. 1994. Restoration of x-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266:288-291.
38. Taccioli, G. E., T. M. Gottlieb, T. Blunt, A. Priestley, J. Demengeot, R. Mizuta, A. R. Lehmann, F. W. Alt, S. P. Jackson, and P. A. Jeggo. 1994. Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science 265:1442-1445.
39. Thode, S., A. Schäfer, P. Pfeiffer, and W. Vielmelter. 1990. A novel pathway of DNA end-to-end joining. Cell 60:921-928.
40. Weis-Garcia, F., E. Besmer, D. J. Sawchuk, W. Yu, Y. Hu, S. Cassard, M. C. Nussenzweig, and P. Cortes. 1997. V(D)J recombination: in vitro coding joint formation. Mol. Cell. Biol. 17:6379-6385.
41. Yaneva, M., T. Kowalewski, and M. R. Lieber. 1997. Interaction of DNA-dependent protein kinase with DNA and with Ku: biochemical and atomic-force microscopy studies. EMBO J. 16:5098-5112.