Ku proteins join DNA fragments as shown by atomic force microscopy

Cancer Research

Volumn 57, Issue 8, 1997, Pages 1412-1415

  Pang, Dalong ^a,b   Yoo, Sunghan ^c   Dynan, William S ^c   Jung, Mira ^a   Dritschilo, Anatoly ^a  

^a GEORGETOWN UNIVERSITY MEDICAL CENTER   (United States)

^b GEORGE WASHINGTON UNIVERSITY   (United States)

^c MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN;

ARTICLE; ATOMIC FORCE MICROSCOPY; DNA STRAND; HUMAN; MOLECULAR INTERACTION; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION;

ANTIGENS, NUCLEAR; DNA; DNA HELICASES; DNA-BINDING PROTEINS; MICROSCOPY; NUCLEAR PROTEINS; PLASMIDS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH;

EID: 0030907318     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Mimori, T., Akizuki, M., Yamagata, H., Inada, S., Yoshida, S., and Homma, M. Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap. J. Clin. Invest., 68: 611-620, 1981.
2. Mimori, T., Hardin, J. A., and Steitz, J. A. Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with phenmatic disorders. J. Biol. Chem., 261: 2274-2278, 1986.
3. Mimori, T., and Hardin, J. A. Mechanism of interaction between Ku Protein and DNA. J. Biol. Chem., 261: 10375-10379, 1986.
4. de Vries, E., van Driel, W., Bergsma, W. G., Arnberg, A. C., and van der Vliet, P. C. HeLa nuclear protein recognizing DNA termini and translocating on DNA forming a regular DNA-multimeric protein complex. J. Mol. Biol., 208; 65-78, 1989.
5. Blier, P. R., Griffith, A. J., Craft, J., and Hardin, J. A. Binding of Ku Protein to DNA. J. Biol. Chem., 268: 7594-7601, 1993.
6. Paillard, S., and Strauss, F. Analysis of the mechanism of interaction of simian Ku protein with DNA. Nucleic Acids Res., 19: 5619-5624, 1991.
7. Falzon M., Fewell J. W., and Kuff, E. L. EBP-80, a transcription factor closely resembling the human autoantigen Ku, recognizes single-to-double strand transitions in DNA. J. Biol. Chem., 268: 10546-10552, 1993.
8. Anderson, C. W. DNA damage and the DNA-activated protein kinase. Trends Biochem. Sci., 18: 433-437, 1993.
9. Getts, R. C., and Stamato, T. D. Absence of a Ku-like DNA end binding activity in the xrs double-strand DNA repair-deficient mutant. J. Biol. Chem., 269: 159981- 15984, 1994.
10. Smider, V., Rathmell, W. K., Lieber, M. R., and Chu, G. Restoration of x-ray resistance and V(D)J recombination in mutant cells by Ku cDNA, Science (Washington DC), 266: 288-291, 1994.
11. Rathmell, W. K., and Chu, G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA, 91: 7623-7627, 1994.
12. Taccioli, G., Gottlieb, T. M., Blunt, T., Priestly, A., Demengeot, J., Mizu, R., Lehmann, A., Alt, F. W., Jackson, S. P., and Jeggo, P. A. Ku80 product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science (Washington DC), 265: 1442-1445, 1994.
13. Dvir, A., Peterson, S. R., Knuth, M. W., Lu, H., and Dynan, W. S. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II Proc. Natl. Acad. Sci. USA, 89: 11920-11924, 1992.
14. Gottlieb, T. M., and Jackson, S. P. The DNA-dependent protein kinase requirement for DNA ends and association with Ku antigen. Cell, 72: 131-142, 1993.
15. Milne, G. T., Jin, S., Shannon, K. B., and Weaver, D. T. Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae. Mol. Cell, Biol., 16: 4189-4198, 1996.
16. Boulton, S. J., and Jackson, S. P. Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathways. EMBO J., 15: 5093-5103, 1996.
17. Pang, D., Popescu, G. F., Rodgers, J., Berman, B. L., and Dritschilo, A. Atomic force microscopy investigation of radiation induced DNA strand breaks. Scanning Microsc., in press, 1996.
18. Ono, M., Tucker, P. W., and Capra, J. D. Production and characterization of recombinant human Ku antigen. Nucleic Acids Res., 22: 3918-3924, 1994.