The amino-terminal domain of Pseudomonas aeruginosa ExoS disrupts actin filaments via small-molecular-weight GTP-binding proteins

Molecular Microbiology

Volumn 32, Issue 2, 1999, Pages 393-401

  Pederson, Kristin J ^a   Vallis, Amy J ^a   Aktories, Klaus ^b   Frank, Dara W ^a   Barbierl, Joseph T ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CYTOTOXIN; GUANINE NUCLEOTIDE BINDING PROTEIN; LUCIFERASE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; RHO FACTOR; TRYPAN BLUE;

ACTIN FILAMENT; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENETICS; CARBOXY TERMINAL SEQUENCE; CHO CELL; CONTROLLED STUDY; CYTOSKELETON; DEAMINATION; GENE DELETION; GENE MAPPING; GENE REARRANGEMENT; GENETIC TRANSFECTION; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PSEUDOMONAS AERUGINOSA; REPORTER GENE; SIGNAL TRANSDUCTION;

EID: 0032931551     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01359.x     Document Type: Article

References (31)

1. Aktories, K. (1997) Bacterial toxins that target Rho proteins. J Clin Invest 99: 827-829.
2. Coburn, J. (1992) Pseudomonas aeruginosa exoenzyme S. Curr Topics Microbiol Immunol 175: 133-143.
3. Coburn, J., Dillion, T.S., Iglewski, B.H., and Gill, D.M. (1989a) Exoenzyme S of Pseudomonas aeruginosa ADP-ribosylates the intermediate filament protein vimentin. Infect Immun 57: 996-998.
4. Coburn, J., and Gill, D.M. (1991) ADP-ribosylation of p21ras and related proteins by Pseudomonas aeruginosa exoenzyme S. Infect Immun 59: 4259-4262.
5. Coburn, J., Wyatt, R.T., Iglewski, B.H., and Gill, D.M. (1989b) Several GTP-binding proteins, including p21c-H-ras are preferred substrates of Pseudomonas aeruginosa exoenzyme S. J Biol Chem 264: 9004-9008.
6. Cornelis, G.R., and Wolf-Watz, H. (1997) The Yersinia yop virulon: a bacterial system for subverting eukaryotic cells. Mol Microbiol 23: 861-867.
7. Fiorentini, C., Fabbri, A., Flatau, G., Donelli, G., Matarrese, P., Lemichez, E., et al. (1997) Escherichia coli cytotoxic necrotizing factor 1 (CNF1), a toxin that activates the Rho GTPase. J Biol Chem 272: 19532-19537.
8. Flatau, G., Lemichez, E., Gauthier, M., Chardin, P., Paris, S., Florentini, C., et al. (1997) Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine. Nature 387: 729-733.
9. Frank, D. (1997) The exoenzyme S regulon of Pseudomonas aeruginosa. Mol Microbiol 26: 621-629.
10. Frithz-Lindsten, E., Du, Y., Rosqvist, R., and Forsberg, A. (1997) Intracellular targeting of exoenzyme S of Pseudomonas aeruginosa via type III-dependent translocation induces phagocytosis resistance, cytotoxicity and disruption of actin microfilaments. Mol Microbiol 25: 1125-1139.
11. Fu, Y., and Galan, J.E. (1998) The Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton. Mol Microbiol 27: 359-368.
12. Ganesan, A.K., Frank, D.W., Misra, R.P., Schmidt, G., and Barbieri, J.T. (1998) Pseudomonas aeruginosa exoenzyme S ADP-ribosylates ras at multiple sites. J Biol Chem 273: 7332-7337.
13. Iglweski, B., Sadoff, H.J., Bjorn, M.J., and Maxwell, E.S. (1978) Pseudomonas aeruginosa exoenzyme S: an adenosine diphosphate ribosyltransferase distinct from toxin A. Proc Natl Acad Sci USA 75: 3211-3215.
14. Kaniga, K., Uralil, J., Bliska, J.B., and Galan, J.E. (1996) A secreted protein tyrosine phosphatase with modular effector domains in the bacterial pathogen Salmonella typhimurium. Mol Microbiol 21: 633-641.
15. Knight, D.A., and Barbieri, J.T. (1997) Ecto-ADP-ribosyltransferase activity of Pseudomonas aeruginosa exoenzyme S. Infect Immun 65: 3304-3309.
16. Knight, D.A., Finck-Barbançon, V., Kulich, S.M., and Barbieri, J.T. (1995) Functional domains of Pseudomonas aeruginosa exoenzyme S. Infect Immun 63: 3182-3186.
17. Krueger, K.M., and Barbieri, J.T. (1995) The family of bacterial ADP-ribosylating exotoxins. Clin Microbiol Rev 8: 34-47.
18. Kulich, S.M., Yahr, T.L., Mende-Mueller, L.M., Barbieri, J.T., and Frank, D.W. (1994) Cloning the structural gene for the 49-kDa form of exoenzyme S (exoS) from Pseudomonas aeruginosa strain 388. J Biol Chem 269: 10431-10437.
19. Liu, S., Kulich, S.M., and Barbieri, J.T. (1996) Identification of glutamic acid 381 as a candidate active site residue of Pseudomonas aeruginosa exoenzyme S. Biochemistry 35: 2754-2758.
20. McGuffie, E.M., Frank, D.W., Vincent, T.S., and Olson, J.C. (1998) Modification of Ras in eukaryotic cells by Pseudomonas aeruginosa exoenzyme S. Infect Immun 66: 2607-2613.
21. Mescas, J., Raupach, B., and Falkow, S. (1998) The Yersinia Yops inhibit invasion of Listeria, Shigella, and Edwardsiella, but not Salmonella into epithelial cells. Mol Microbiol 28: 1269-1281.
22. Olson, J.C., McGuffie, E.M., and Frank, D.W. (1997) Effects of differential expression of the 49-kilodalton exoenzyme S by Pseudomonas aeruginosa on cultured eukaryotic cells. Infect Immun 65: 248-256.
23. Pederson, K.J., and Barbieri, J.T. (1998) Intracellular expression of the ADP-ribosyltransferase domain of Pseudomonas exoenzyme S is cytotoxic to eukaryotic cells. Mol Microbiol 30: 751-760.
24. Rosqvist, R., Forsberg, A., Rimpilainen, M., Bergman, T., and Wolf-Watz, H. (1990) The cytotoxic protein YopE of Yersinia obstructs the primary host defense. Mol Microbiol 4: 657-667.
25. Rosqvist, R., Forsberg, A., and Wolf-Watz, H. (1991) Intracellular targeting of the Yersinia YopE cytotoxin in mammalian cells induces actin microfilament disruption. Infect Immun 59: 4562-4569.
26. Salyers, A., and Whitt, D.D. (eds) (1994) Pseudomonas aeruginosa. In Bacterial Pathogenesis: A Molecular Approach. Washington, DC: American Society for Microbiology Press.
27. Schmidt, G., Sehr, P., Wilm, M., Selzer, J., Mann, M., and Aktories, K. (1997) GIn-63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1. Nature 387: 725-729.
28. Sherris, J.C. (ed.) (1990) Medical Microbiology: an Introduction to Infectious Diseases, 2nd edn. New York: Elsevier.
29. Wilson, B.A., and Collier, R.J. (1992) Diphtheria toxin and Pseudomonas aeruginosa exotoxin A: active site structure and enzymic mechanism. Curr Topics Microbiol Immun 175: 133-141.
30. Yahr, T.L., Barbieri, J.T., and Frank, D.W. (1996a) Genetic relationship between the 53- and 49-kilodalton forms of exoenzyme S from Pseudomonas aeruginosa. J Bacteriol 178: 1412-1419.
31. Yahr, T.L., Goranson, J., and Frank, D.W. (1996b) Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway. Mol Microbiol 22: 991-1003.