Purification of a highly modified RNA-aptamer. Effect of complete denaturation during chromatography on product recovery and specific activity

Journal of Chromatography B: Biomedical Sciences and Applications

Volumn 726, Issue 1-2, 1999, Pages 237-247

  Bridonneau, Philippe ^a   Bunch, Steve ^b   Tengler, Robert ^a   Hill, Ken ^a   Carter, Jeff ^b   Pieken, Wolfgang ^b   Tinnermeier, David ^a   Lehrman, Russ ^c   Drolet, Daniel W ^a  

^a GILEAD SCIENCES   (United States)

^b Proligo LLC   (United States)

^c NEKTAR THERAPEUTICS   (United States)

Author keywords

RNA aptamers

Indexed keywords

ARTICLE; CHROMATOGRAPHY; DENATURATION; DRUG PURIFICATION; DRUG STABILITY; ION EXCHANGE CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN RNA BINDING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; RNA ANALYSIS; TECHNIQUE; TEMPERATURE;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; ENDOTHELIAL GROWTH FACTORS; LYMPHOKINES; NUCLEIC ACID DENATURATION; RNA; SPECTROPHOTOMETRY, ULTRAVIOLET; VASCULAR ENDOTHELIAL GROWTH FACTOR A; VASCULAR ENDOTHELIAL GROWTH FACTORS;

EID: 0032918498     PISSN: 13872273     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4347(99)00037-7     Document Type: Article

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