The SSCP phenomenon: Addition of HEPES buffer dramatically affects electrophoretic mobility

BioTechniques

Volumn 25, Issue 1, 1998, Pages 50-56

  Liu, Qiang ^a   Sommer, Steve S ^a,b  

^a CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^b Div of Molecular Medicine ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER;

ARTICLE; AUTORADIOGRAPHY; DNA DETERMINATION; ELECTROPHORESIS; ELECTROPHORETIC MOBILITY; GENE AMPLIFICATION; GENE MUTATION; HUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 0031820586     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98251bm11     Document Type: Article

References (7)

1. Cotton, R.G.H. 1997.Mutation Detection, p. 32-58. Oxford University Press, New York.
2. Liu, Q., J. Feng and S.S. Sommer. 1996. Bidirectional dideoxy fingerprinting (Bi-ddF): a rapid method for quantitative detection of mutations in genomic regions of 300-600 bp. Hum. Mol. Genet. 5:107-114.
3. Liu, Q. and S.S. Sommer. 1994. Parameters affecting the sensitivities of dideoxy fingerprinting and SSCP. PCR Methods Appl. 4:97-108.
4. Liu, Q. and S.S. Sommer. 1995. Restriction endonuclease fingerprinting (REF): a sensitivie method for screening mutations in long, contiguous segments of DNA. BioTechniques 18:470-477.
5. Orita, M., H. Iwahana, H. Kanazawa, K. Hayashi and T. Sekiya. 1989. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA 86:2766-2770.
6. Sarkar, G. and S.S. Sommer. 1992. Doublestranded DNA segments can efficiently prime the amplification of human genomic DNA. Nucleic Acids Res. 20:4937-4938.
7. Yoshitake, S., B.C. Schach, D.C. Foster, E.W. Davie and K. Karachi. 1985. Nucleotide sequence of the gene for human factor IX (anti-hemophilic factor B). Biochemistry 24:3736-3750.