In a blinded analysis, restriction endonuclease fingerprinting detects all the mutations in a 1.9-kb segment

BioTechniques

Volumn 23, Issue 5, 1997, Pages 836-839

  Liu, Qiang ^a   Feng, Jinong ^a   Sommer, Steve S ^a,b  

^a MAYO CLINIC   (United States)

^b CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RESTRICTION ENDONUCLEASE;

ARTICLE; AUTORADIOGRAPHY; CONTROLLED STUDY; DNA FINGERPRINTING; EXON; HEMOPHILIA B; HUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; RESTRICTION SITE; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 0030667271     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97235bm15     Document Type: Article

References (8)

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2. Liu, Q. and S.S. Sommer. 1994. Parameters affecting the sensitivities of dideoxy fingerprinting and SSCP. PCR Methods Appl. 4:97-108.
3. Liu, Q. and S.S. Sommer. 1995. Restriction endonuclease fingerprinting (REF): a sensitive method for screening mutations in long, contiguous segments of DNA. BioTechniques 18:470-477.
4. Orita, M., H. Iwahana, H. Kanazawa, K. Hayashi and T. Sekiya. 1989. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA 86:2766-2770.
5. Sarkar, G., S. Kapelner and S.S. Sommer. 1990. Formamide can dramatically improve the specificity of PCR. Nucleic Acids Res. 18:7465.
6. Sarkar, G., H. Yoon and S.S. Sommer. 1992. Dideoxy fingerprinting (ddF): a rapid and efficient screen for the presence of mutations. Genomics 13:441-443.
7. Savitsky, K., A. Bar-Shira, S. Gilad, G. Rotman, Y. Ziv, L. Vanagaite, D.A. Tagle, S. Smith et al. 1995. A single ataxia telangiectasia gene with a product similar to P1-3 kinase. Science 268:1749-1753.
8. Yoshitake, S., B.G. Schach, D.C. Foster, E.W. Davie and K. Kurachi. 1985. Nucleotide sequence of the gene for human factor IX (anti-hemophilic factor B). Biochemistry 24:3736-3750.