Cloning and functional expression of the calmodulin gene from Toxoplasma gondii

Molecular and Biochemical Parasitology

Volumn 99, Issue 2, 1999, Pages 295-299

  Seeber, Frank ^a   Beuerle, Barbara ^b   Schmidt, H H H W ^b  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Calmodulin; Expressed sequence tag; Recombinant expression

Indexed keywords

CALMODULIN;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; CONTROLLED STUDY; GENE EXPRESSION REGULATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SHORT SURVEY; TOXOPLASMA GONDII;

TOXOPLASMA GONDII;

EID: 0032913345     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(99)00030-4     Document Type: Article

References (27)

1. Cheung W.Y. Calcium and Cell Function: Calmodulin. 1:1980;Academic Press, New York.
2. Clapham D.E. Calcium signaling. Cell. 80:1995;259-268.
3. James P., Vorherr T., Carafoli E. Calmodulin-binding domains: just two faced or multi-faceted? Trends Biochem. Sci. 20:1995;38-42.
4. Endo T., Sethi K.K., Piekarski G. Toxoplasma gondii: calcium ionophore A23187-mediated exit of trophozoites from infected murine macrophages. Exp. Parasitol. 53:1982;179-188.
5. Moreno S.N., Zhong L. Acidocalcisomes in Toxoplasma gondii tachyzoites. Biochem. J. 313:1996;655-659.
6. 2+-dependence of conoid extrusion in Toxoplasma gondii tachyzoites. J. Euk. Microbiol. 43:1996;120-127.
7. Pezzella N., Bouchot A., Bonhomme A. et al. Involvement of calcium and calmodulin in Toxoplasma gondii tachyzoite invasion. Eur. J. Cell Biol. 74:1997;92-101.
8. Pingret L., Millot J.M., Sharonov S., Bonhomme A., Manfait M., Pinon J.M. Relationship between intracellular free calcium concentrations and the intracellular development of Toxoplasma gondii. J. Histochem. Cytochem. 44:1996;1123-1129.
9. 2+ flux causes egress of parasites from the parasitophorous vacuole. Exp. Parasitol. 87:1997;88-97.
10. Holfels E., McAuley J., Mack D., Milhous W.K., McLeod R. In vitro effects of artemisinin ether, cycloguanil hydrochloride (alone and in combination with sulfadiazine), quinine sulfate, mefloquine, primaquine phosphate, trifluoperazine hydrochloride, and verapamil on Toxoplasma gondii. Antimicrob. Agents Chemother. 38:1994;1392-1396.
11. Cowman A.F., Galatis D. Plasmodium falciparum: the calmodulin gene is not amplified or overexpressed in chloroquine resistant or sensitive isolates. Exp. Parasitol. 73:1991;269-275.
12. Robson K.J., Jennings M.W. The structure of the calmodulin gene of Plasmodium falciparum. Mol. Biochem. Parasitol. 46:1991;19-34.
13. Koller M., Schnyder B., Strehler E.E. Structural organization of the human CAMIII calmodulin gene. Biochem. Biophys. Acta. 1087:1990;180-189.
14. Ajioka J.W., Boothroyd J.C., Brunk B.P. et al. Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa. Genome Res. 8:1998;18-28.
15. Seeber F. Consensus sequence of translational initiation sites from Toxoplasma gondii genes. Parasitol. Res. 83:1997;309-311.
16. Schmidt T.G.M., Skerra A. One-step affinity purification of bacterially produced proteins by means of the strep-tag and immobilized recombinant core steptavidin. J. Chromatogr. A676:1994;337-345.
17. Rasmussen C.D., Simmen R.C.M., MacDougall E.A., Means A.R. Methods for analyzing bovine papilloma virus-based calmodulin expression vectors. Meth. Enzymol. 139:1987;642-654.
18. Geiser J.R., Tuinen D.V., Brockerhoff S.E., Neff M.N., Davis T.N. Can calmodulin function without binding calcium? Cell. 65:1991;949-959.
19. Stirling D.A., Petrie A., Pulford D.J., Paterson D.T.W., Stark M.J. Protein A-calmodulin fusions: a novel approach for investigating calmodulin function in yeast. Mol. Microbiol. 6:1991;703-713.
20. Szymanska G., O'Connor M.B., O'Connor C.M. Construction of an epitope-tagged calmodulin useful for the analysis of calmodulin-binding proteins: addition of a hemagglutinin epitope does not affect calmodulin-dependent activation of smooth muscle myosin light chain kinase. Anal. Biochem. 252:1997;96-105.
21. 2+-dependent high-affinity complex formation between calmodulin and melittin. Biochem. J. 209:1983;269-272.
22. Bredt D.S., Schmidt H.H.H.W. The citrulline assay. Feelisch M., Stamler J.S. Methods in Nitric Oxide Research. 1996;249-255 Wiley, Chichester.
23. Boothroyd J.C., Black M., Kim K. et al. Forward and reverse genetics in the study of the obligate, intracellular parasite Toxoplasma gondii. Adolph K. Methods in Molecular Genetics. 6:1995;3-29 Academic Press, San Diego.
24. Seeber F., Dubremetz J.F., Boothroyd J. Analysis of Toxoplasma gondii stably transfected with a transmembrane variant of its major surface protein, SAG1. J. Cell Sci. 111:1998;23-29.
25. Seeber F., Boothroyd J.C. E. coli β-galactosidase as an in vitro and in vivo reporter enzyme and stable transfection marker in the intracellular protozoan parasite Toxoplasma gondii. Gene. 169:1996;39-45.
26. Hulen D., Baron A., Salisbury J., Clarke M. Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum. Cell Motil. Cytoskel. 18:1991;113-122.
27. Yasukawa T., Kanei-Ishii C., Maekawa T., Fujimoto J., Yamamoto T., Ishii S. Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin. J. Biol. Chem. 270:1995;25328-25331.