Analysis of Toxoplasma gondii stably transfected with a transmembrane variant of its major surface protein, SAG1

Journal of Cell Science

Volumn 111, Issue 1, 1998, Pages 23-29

  Seeber, Frank ^a,b   Dubremetz, Jean François ^c   Boothroyd, John C ^b  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c INSERM   (France)

Author keywords

GPI anchor; Parasite; SAG1; Toxoplasma gondii

Indexed keywords

MEMBRANE ANTIGEN; MEMBRANE PROTEIN; PARASITE ANTIGEN;

ARTICLE; GENETIC ENGINEERING; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL; TOXOPLASMA GONDII;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, PROTOZOAN; ANTIGENS, SURFACE; CERCOPITHECUS AETHIOPS; GLYCOSYLPHOSPHATIDYLINOSITOLS; HUMANS; MEMBRANE PROTEINS; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; PROTOZOAN PROTEINS; TOXOPLASMA; TRANSFECTION; VERO CELLS;

EID: 0031976083     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Araujo, F., Handman, E. and Remington, J. S. (1980). Use of monoclonal antibodies to detect antigens of Toxaplasma gondii in serum and other body fluids. Infect. Immun. 30, 12-16.
2. Barboni, E., Rivero, B. P, George, A. J. T., Martin, S. R., Renouf, D. V., Hounsell, E. F., Barber. P. C. and Morris, R. J. (1995). The glycophosphatidylinositol anchor affects the conformation of Thy-l protein. J. Cell Sci. 108, 487-497.
3. Barth, A., Müller-Taubenberger, A., Taranto, P. and Gerisch, G. (1994). Replacement of the phospholipid-anchor in the contact site A glycoprotein of D. discoideum by a transmembrane region does not impede cell adhesion but reduces residence time on the cell surface. J. Cell Biol. 124, 205-215.
4. Beckers, C. J., Roos, D. S., Donald, R. G., Luft, B. J., Schwab, J. C., Cao, Y. and Joiner, K. A. (1995). Inhibition of cytoplasmic and organellar protein synthesis in Toxoplasma gondii: implications for the target of macrolide antibiotics. J. Clin. Invest. 95, 367-376.
5. Black, M., Seeber, F., Soldati, D., Kim, K. and Boothroyd, J. C. (1995). Restriction-enzyme-mediated-integration increases transformation frequency and allows efficient co-transfection of multiple unselected markers in Toxoplsma gondii. Mol. Biochem. Parasitol. 74, 55-60.
6. Boothroyd, J. C., Black, M., Kim, K., Pfefferkorn, E. R., Seeber, F., Sibley, L. D. and Soldati, D. (1995). forward and reverse genetics in the study of the obligate, intracellular parasite toxoplasma gondii. In Methods in Molecular Genetics. vol. 6. (ed. K. Adolph), pp. 3-29. Academic Press, San Diego.
7. Braun-Breton, C., Blisnick, T., Barbot, P., Bülow, R., Silva. L. P. D. and Langsley, G. (1992).Plasmodium falciparum and Plasmodium chabaudi: characterization of glycosyiphosphatidylinositol-degrading activities. Exp. Parasitol. 74, 452-462.
8. Brusca, J. S. and Radolf, J. D. (1994). Isolation of integral membrane proteins by phase partitioning with Triton X-l14. Meth. Enzymol. 228, 182-193.
9. Bülow, R. and Boothroyd, J. C. (1991). Protection of mice from fatal Toxoplasma gondii infection by immunization with p30 antigen in liposomes. J. Immunol. 147, 3496-3500.
10. Burg, J. L., Perelman, D., Kasper, L. H., Ware, P. L. and Boothroyd, J. C. (1988). Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J. Immunol. 141, 3584-3591.
11. Dubremetz, J. F., Rodriguez, C. and Ferreira, E. (1985). Toxoplasma gondii: redistribution of monoclonal antibodies on tachyzoites during host cell invasion. Exp. Parasitol. 59, 24-32.
12. Ferguson, M. A. J. (1994). What can GP1 do for you? Parasitol. Today 10, 48-52.
13. Grimwood, J. and Smith, J. E. (1995). Toxoplasma gondii: redistribution of tachyzoite surface protein during host cell invasion and intracellular development. Parasitol. Res. 81, 657-661.
14. Harlow, E. and Lane, D. (1988). Antibodies: a Laboratory Manual. CSH Laboratory, Cold Spring Harbor, NY.
15. Hooper, N. M. and Bashir, A. (1991). Glycosyl-phosphatidylinositol-anchored membrane proteins can be distinguished from transmembrane polypeptideanchored proteins by differential solubilization and temperature-induced phase separation in Triton X-114. Biochem. J. 280, 745-751.
16. Uangumaran, S., Robinson, P. J. and Hoessli, D. C. (1996). Transfer of exogenous glycosyl-phosphatidylinositol (GPI)-linked molecules to plasma membranes. Trends Cell Biol. 6, 163-167.
17. Kasper, L. H., Bradley, M. S. and Pfefferkorn, E. R. (1984). Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. J. Immunol. 132, 443-449.
18. Kasper, L. H. (1987). Isolation and characterization of a monoclonal anti-P30 antibody resistant mutant of Toxoplasma gondii. Parasite Immunol. 9, 433-445.
19. Kasper, L. H., Khan, I. A., Ely, K. H., Buelow, R. and Boothroyd, J. C. (1992). Antigen-specific (p30) mouse CD8+ T cells are cytotoxic against Toxoplasma gondii-infected peritoneal macrophages. J. Immunol 148, 1493-1498.
20. Kemble, G. W., Henis, Y. I. and White, J. M. (1993). GPI- and transmembraneanchored influenza hemaglutinin differ in structure and receptor binding activity. J. Cell Biol. 121, 1253-1265.
21. Khan, I. A., Ely, K. H. and Kasper, L. H. (1991). A purified parasite antigen (p30) mediates CD8+ T cell immunity against fatal Toxoplasma gondii infection in mice. J. Immunol 147, 3501-3506.
22. Kim, K., Soldati, D. and Boothroyd, J. C. (1993). Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. Science 262, 911-914.
23. Kim, K., Bülow, R., Kampmeier, J. and Boothroyd, J. C. (1994). Conformationally appropriate expression of the Toxoplasma antigen SAG 1 (p30) in CHO cells. Infect. Immun. 62, 203-209.
24. Kim, K. and Boothroyd, J. C. (1995). Toxoplasma gondii: stable complementation of sag1 (p30) mutants using SAG1 transfection and fluorescence-activated cell sorting. Exp. Parasitol. 80, 46-53.
25. Kodukula, K., Gerber, L. D., Amthauer, R., Brink, L. and Udenfriend, S. (1993). Biosynthesis of glycosylphosphatidylinositol (GPI)-anchored membrane protins in intact cells: Specific amino acid requirements adjacent to the site of cleavage and GPI attachment. J. Cell Biol. 120, 657-664.
26. Lee, J. D., Kravchenko, V., Kirkland, T. N., Han, J., Mackman, N., Moriatry, A., Letureq, D., Tobias, P. S. and Ulevitch, R. J. (1993). Glycosylphosphatidylmositol-anchored or integral membrane forms of CD14 mediate identical cellular reponses to endotoxin. Proc. Nat. Acad. Sci. USA 90, 9930-9934.
27. Letillois, M. F., Laigle, V., Santoro, F., Micoud, M. and Chumpitazi, B. F. F. (1995). Toxoplasma gondii surface antigen-1 in sera of HIV-infected patients as an indicator of reactivated toxoplasmosis. Eur. J. Clin. Microbiol. Infect. Dis. 14, 899-903.
28. Liszewski, M. K., Post, T. W. and Atkinson, J. P. (1991). Membrane cofactor protein (MCP or CD46): newest member of the regulators of complement activation gene cluster. Annu. Rev. Immunol. 9, 431-455.
29. Lublin, D. M., Liszewski, M. K., Post, T. W., Arce, M. A., Le Beau, M. M., Rebentisch, M. B., Lemons, L. S., Seya, T. and Atkinson, J. P. (1988). Molecular cloning and chromosomal localization of human membrane cofactor protein (MCP) Evidence for inclusion in the multigene family of complement-regulatory proteins. J. Exp. Med. 168, 181-194.
30. Lublin, D. M. and Coyne, K. E. (1991). Phospholipid-anchored and transmembrane versions of either decay-accelerating factor or membrane cofactor protein show equal efficiency in protection from complementmediated cell damage. J. Exp. Med. 174, 35-44.
31. Luft, B. J. and Remington, J. S. (1992). Toxoplasmic encephalitis in AIDS. Clin. Infect. Dis. 15, 211-222.
32. McConville, M. J. and Ferguson, M. A. J. (1993). The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes. Biochem. J. 294, 305-324.
33. Metz, C. N., Brunner, G., Choi-Muira, N. H., Nguyen, H., Gabrilove, J. , Caras, I. W., Altszuler, N., Rifkin, D. B., Wilson, H. L. and Davitz, M. A. (1994). Release of GPI-anchored membrane proteins by a cell-associated GPI-specific phospholipase D. EMBO J. 13, 1741-1751.
34. Mineo, J. R., McLeod, R., Mack, D., Smith, J., Khan, I. A., Ely, K. H. and Kasper, L. H. (1993). Antibodies to Toxoplasma gondii major surface protein (SAG-1, P30) inhibit infection of host cells and are produced in murine intestine after peroral infection. J. Immunol. 150, 3951-3964.
35. Mineo, J. R. and Kasper, L. H. (1994). Attachment of Toxoplasma gondii to host cells involves major surface protein. SAG-1 (P30). Exp. Parasitol. 79, 1120.
36. Moran, P. and Caras, I. W. (1994). Requirements for glycosylphosphatidylinositol attachment are similar but not identical in mammalian cells and parasitic protozoa. J. Cell Biol. 125, 333-343.
37. Nagel, S. and Boothroyd, J. C. (1989). The major surface antigen, P30, of Toxoplasma gondii is anchored by a glycolipid. J. Biol. Chem. 264, 5569-5574.
38. Odenthal-Schnittler, M., Tomavo, S., Becker, D., Dubremetz, J. F. and Schwarz, R. T. (1993). Evidence for N-linked glycosylation in 'Toxoplasma gondii. Biochem. J. 291, 713-721.
39. Quemeneur, K. and Simonnet, F. (1995). Increased sensitivity of autoradiography and fluorography by membrane blotting. BioTechniqes 18, 100-103.
40. Robinson, P. J., Millrain, M., Antoniou, J., Simpson, E. and Mellor, A. L. (1989). A glycophospholipid anchor is required for Qa-2-mediated T cell activation. Nature 342, 85-87.
41. Kodriguez, C., Afchain, D., Capron, A., Dissous, C. and Santoro, F. (1985). Major surface protein of Toxoplasma gondii (p30) contains an immunodominant region with repetitive epitopes. Eur. J. Immunol. 15, 747-749.
42. Santoro, F., Charif, H. and Capron, A. (1986) The immunodominant epitope of the major membrane tachyzoite protein (P30) of Toxoplasma gondii. Parasite Immunol. 8, 631-639.
43. Tomavo, S., Schwarz, R. T. and Dubremetz, J. F. (1989). Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens. Mol. Cell. Biol. 9, 4576-4580.
44. Tomavo, S., Martinage, A. and Dubremetz, J. F. (1992). Phosphorylation of Toxoplasma gondii major surface antigens. Parasitol. Res. 78, 541-544.
45. Waneck, G. L., Stein, M. E. and Flavell, R. A. (1988). Conversion of a Planchored protein to an integral membrane protein by a single amino acid mutation. Science 241, 697-700.
46. Wong, S. Y. and Remington, J. S. (1993) Biology of Toxoplasma gondii. AIDS 7, 299-316.
47. Xie, M. and Low, M. G. (1994). Expression and secretion of glycosylphosphatidylinositol-specific phospholipase D by myeloid cell lines. Biochem. J. 297, 547-554.