메뉴 건너뛰기




Volumn 162, Issue 1, 1999, Pages 254-262

Intranasal immunization with plasmid DNA-lipid complexes elicits mucosal immunity in the female genital and rectal tracts

Author keywords

[No Author keywords available]

Indexed keywords

LIPID; LUCIFERASE; PLASMID DNA;

EID: 0032909237     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (130)

References (60)
  • 1
    • 0029039777 scopus 로고
    • Neutralisation of HIV-1 by secretory IgA induced by oral immunization with a new macromolecular multcomponent peptide vaccine candidate
    • K.
    • Bukawa, H., K.-I. Sekigawa, K. Hamajima, K., J. Fukushima, Y. Yamada, H. Kiyono, and K. Okuda. 1995. Neutralisation of HIV-1 by secretory IgA induced by oral immunization with a new macromolecular multcomponent peptide vaccine candidate. Nat. Med. 1:681.
    • (1995) Nat. Med. , vol.1 , pp. 681
    • Bukawa, H.1    Sekigawa, K.-I.2    Hamajima, K.3    Fukushima, J.4    Yamada, Y.5    Kiyono, H.6    Okuda, K.7
  • 4
    • 0028785243 scopus 로고
    • Antiviral cytotoxic T-lymphocytes in the vaginal mucosa of Simian immunodeficiency virus infected rhesus macaques
    • Lohman, B. L., C. J. Miller, and M. B. McChesney. 1995. Antiviral cytotoxic T-lymphocytes in the vaginal mucosa of Simian immunodeficiency virus infected rhesus macaques. J. Immunol. 155:5855.
    • (1995) J. Immunol. , vol.155 , pp. 5855
    • Lohman, B.L.1    Miller, C.J.2    McChesney, M.B.3
  • 6
    • 0024537039 scopus 로고
    • T-lymphocytes in the genital lymph nodes protect mice from intravaginal infection with herpes simplex virus type 2
    • McDermott, M. R., C. H. Goldsmith, K. L. Rosenthal, and L. J. Brais. 1989. T-lymphocytes in the genital lymph nodes protect mice from intravaginal infection with herpes simplex virus type 2. J. Infect. Dis. 159:460.
    • (1989) J. Infect. Dis. , vol.159 , pp. 460
    • McDermott, M.R.1    Goldsmith, C.H.2    Rosenthal, K.L.3    Brais, L.J.4
  • 7
    • 0028868205 scopus 로고
    • Generation of humoral immune responses against herpes simplex type 2 in the murine female genital tract
    • Milligan, F. N., and D. Bernstein. 1995. Generation of humoral immune responses against herpes simplex type 2 in the murine female genital tract. Virology 206:234.
    • (1995) Virology , vol.206 , pp. 234
    • Milligan, F.N.1    Bernstein, D.2
  • 8
    • 0031714448 scopus 로고    scopus 로고
    • Homing of mononuclear cells from iliac lymph nodes to the genital and rectal mucosa in non-human primates
    • Mitchell, E. A., L. A. Bergmeier, C. Doyle, R. Brookes, Y. Wang, and T. Lehner. 1998. Homing of mononuclear cells from iliac lymph nodes to the genital and rectal mucosa in non-human primates. Eur. J. Immunol. 28:3066.
    • (1998) Eur. J. Immunol. , vol.28 , pp. 3066
    • Mitchell, E.A.1    Bergmeier, L.A.2    Doyle, C.3    Brookes, R.4    Wang, Y.5    Lehner, T.6
  • 9
    • 0000173662 scopus 로고
    • Efficacy of live attenuated and whole-inactivated SIV vaccines against intravenous and vaginal challenge
    • F. Brown, R. M. Chanock, and R. A. Lerner, eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
    • Marthas, M. S., S. Sutjipto, C. Miller, J. Higgins, H. Kiyono, J. McGhee, P. Marx, and N. Pederson. 1992. Efficacy of live attenuated and whole-inactivated SIV vaccines against intravenous and vaginal challenge. In Vaccines. F. Brown, R. M. Chanock, and R. A. Lerner, eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. 117.
    • (1992) Vaccines , pp. 117
    • Marthas, M.S.1    Sutjipto, S.2    Miller, C.3    Higgins, J.4    Kiyono, H.5    McGhee, J.6    Marx, P.7    Pederson, N.8
  • 10
    • 0027163425 scopus 로고
    • Mucosal immunity and protection after intranasal immunization with recombinant adenovirus expressing HSV gpB
    • Gallichan, W. S., D. C. Johnson, F. L. Graham, and K. L. Rosenthal. 1993 Mucosal immunity and protection after intranasal immunization with recombinant adenovirus expressing HSV gpB. J. Infect. Dis. 168:622.
    • (1993) J. Infect. Dis. , vol.168 , pp. 622
    • Gallichan, W.S.1    Johnson, D.C.2    Graham, F.L.3    Rosenthal, K.L.4
  • 11
    • 0030587188 scopus 로고    scopus 로고
    • A mucosal or targeted lymph node immunization of macaques with a particulate SIV p27 protein elicits virus specific cytotoxic T lymphocytes in the genito-rectal mucosa and draining lymph nodes
    • Klavinskis, L. S., L. A. Bergmeier, L. Gao, E. Mitchell, and T. Lehner. 1996. A mucosal or targeted lymph node immunization of macaques with a particulate SIV p27 protein elicits virus specific cytotoxic T lymphocytes in the genito-rectal mucosa and draining lymph nodes. J. Immunol 157:2521.
    • (1996) J. Immunol , vol.157 , pp. 2521
    • Klavinskis, L.S.1    Bergmeier, L.A.2    Gao, L.3    Mitchell, E.4    Lehner, T.5
  • 13
    • 0030782958 scopus 로고    scopus 로고
    • CTL responses induced by a single immunization with peptide encapsulated in biodegradable microparticles
    • Partidos, C., D. P. Vohra, D. Joones, G. Farrar, and M. W. Steward. 1997. CTL responses induced by a single immunization with peptide encapsulated in biodegradable microparticles. J Immunol. Methods 206:143.
    • (1997) J Immunol. Methods , vol.206 , pp. 143
    • Partidos, C.1    Vohra, D.P.2    Joones, D.3    Farrar, G.4    Steward, M.W.5
  • 14
    • 0029131261 scopus 로고
    • Induction of humoral and cellular immune responses to the human immunodeficiency type 1 virus in non-human primates by in vivo DNA inoculation
    • Wang, B., J. Boyer, V. Srikantan, K. Ugen, L. Gilbert, C. Phan, K. Dang, M. Merva, M. G. Agadjanyan, M. Newman, et al. 1995. Induction of humoral and cellular immune responses to the human immunodeficiency type 1 virus in non-human primates by in vivo DNA inoculation. Virology 211:102.
    • (1995) Virology , vol.211 , pp. 102
    • Wang, B.1    Boyer, J.2    Srikantan, V.3    Ugen, K.4    Gilbert, L.5    Phan, C.6    Dang, K.7    Merva, M.8    Agadjanyan, M.G.9    Newman, M.10
  • 17
    • 0029051321 scopus 로고
    • DNA-mediated immunization to the hepatitis B surface antigen in mice: Aspects of the humoral response mimics hepatitis B viral infection in humans
    • Michael, M. L., H. L. Davis, M. Schleef, M. Mancini, P. Tiollais, and R. E. Whalen. 1995. DNA-mediated immunization to the hepatitis B surface antigen in mice: aspects of the humoral response mimics hepatitis B viral infection in humans. Proc. Natl. Acad. Sci. USA 92:5307.
    • (1995) Proc. Natl. Acad. Sci. USA , vol.92 , pp. 5307
    • Michael, M.L.1    Davis, H.L.2    Schleef, M.3    Mancini, M.4    Tiollais, P.5    Whalen, R.E.6
  • 18
    • 0028244596 scopus 로고
    • Vaccination with a plasmid vector carrying the rabies glycoprotein gene induces protective immunity against rabies virus
    • Xiang, Z. Q., M. Spitalnik, M. Tran, W. H. Wunner, J. Cheng, and H. C. J. Ertl. 1994. Vaccination with a plasmid vector carrying the rabies glycoprotein gene induces protective immunity against rabies virus. Virology 199:132.
    • (1994) Virology , vol.199 , pp. 132
    • Xiang, Z.Q.1    Spitalnik, M.2    Tran, M.3    Wunner, W.H.4    Cheng, J.5    Ertl, H.C.J.6
  • 19
    • 0026579897 scopus 로고
    • Genetic immunization is a simple method for eliciting an immune response
    • Tang, D. C., M. Devil, and S. A. Johnston. 1992. Genetic immunization is a simple method for eliciting an immune response. Nature 356:152.
    • (1992) Nature , vol.356 , pp. 152
    • Tang, D.C.1    Devil, M.2    Johnston, S.A.3
  • 20
    • 0029037299 scopus 로고
    • Use of DNA's expressing HIV env and non-infectious HIV-1 particles to raise antibody responses in mice
    • Lu, S., J. C. Santoro, D. H. Fuller, J. R. Haynes, and H. L. Robinson. 1995. Use of DNA's expressing HIV env and non-infectious HIV-1 particles to raise antibody responses in mice. Virology 209:147.
    • (1995) Virology , vol.209 , pp. 147
    • Lu, S.1    Santoro, J.C.2    Fuller, D.H.3    Haynes, J.R.4    Robinson, H.L.5
  • 22
    • 0028073550 scopus 로고
    • DNA inoculation induces protective in vivo immune responses against cellular challenge with HIV-1 antigen expressing cells
    • Wang, B. M. Merva, K. Dang, K. E. Ugen, J. Boyer, W. W. Williams, and D. B. Weiner. 1994. DNA inoculation induces protective in vivo immune responses against cellular challenge with HIV-1 antigen expressing cells. AIDS Res. Hum. Retroviruses 10(Suppl. 2):S35.
    • (1994) AIDS Res. Hum. Retroviruses , vol.10 , Issue.SUPPL. 2
    • Wang1    Merva, B.M.2    Dang, K.3    Ugen, K.E.4    Boyer, J.5    Williams, W.W.6    Weiner, D.B.7
  • 23
    • 0015097333 scopus 로고
    • An enriched source of precursors for IgA-producing immunocytes in the rabbit
    • Craig, C. W., and J. J. Cebra. 1971. An enriched source of precursors for IgA-producing immunocytes in the rabbit. J. Exp. Med. 134:188.
    • (1971) J. Exp. Med. , vol.134 , pp. 188
    • Craig, C.W.1    Cebra, J.J.2
  • 24
    • 0016740954 scopus 로고
    • Repopulation with IgA-containing cells of bronchial and intestinal lamina propria after transfer of homologous Peyer's patches and bronchial lymphocytes
    • Rudzik, O., R. L. Clancy, D. Y. E. Percy, R. P. Day, and J. Bienenstock, 1975. Repopulation with IgA-containing cells of bronchial and intestinal lamina propria after transfer of homologous Peyer's patches and bronchial lymphocytes. J. Immunol. 114:1599.
    • (1975) J. Immunol. , vol.114 , pp. 1599
    • Rudzik, O.1    Clancy, R.L.2    Percy, D.Y.E.3    Day, R.P.4    Bienenstock, J.5
  • 26
    • 0344856570 scopus 로고
    • Evidence for a common mucosal immunologic system. I. Migration of B Immunoblasts into intestinal, respiratory and genital tissues
    • McDermott, M. R., and J. Bienenstock. 1975. Evidence for a common mucosal immunologic system. I. Migration of B Immunoblasts into intestinal, respiratory and genital tissues. J. Immunol. 122:1992.
    • (1975) J. Immunol. , vol.122 , pp. 1992
    • McDermott, M.R.1    Bienenstock, J.2
  • 27
    • 0018677145 scopus 로고
    • Organ and isotope distribution of plasma cells producing specific antibody after oral immunization: Evidence for a generalized secretory immune system
    • Weisz-Carrington, P., N. E. Roux, M. E. McWilliams, J. M. Phillips-Quagliatta, and M. E. Lamm. 1979. Organ and isotope distribution of plasma cells producing specific antibody after oral immunization: evidence for a generalized secretory immune system. J. Immunol. 123:1705.
    • (1979) J. Immunol. , vol.123 , pp. 1705
    • Weisz-Carrington, P.1    Roux, N.E.2    McWilliams, M.E.3    Phillips-Quagliatta, J.M.4    Lamm, M.E.5
  • 30
    • 0029877628 scopus 로고    scopus 로고
    • Converting an alcohol to an amine in a cationic lipid dramatically alters the co-lipid requirement, cellular transfection activity and the ultrastructure of DNA-cytofectin complexes
    • Wheeler, C. J., L. Sukhu, G. Yang, Y. Tsai, C. Bustamente, P. Felgner, J. Norman, and M. Manthorpe. 1996. Converting an alcohol to an amine in a cationic lipid dramatically alters the co-lipid requirement, cellular transfection activity and the ultrastructure of DNA-cytofectin complexes. Bioch. Biophys. Acta 1280:1.
    • (1996) Bioch. Biophys. Acta , vol.1280 , pp. 1
    • Wheeler, C.J.1    Sukhu, L.2    Yang, G.3    Tsai, Y.4    Bustamente, C.5    Felgner, P.6    Norman, J.7    Manthorpe, M.8
  • 32
    • 0028686609 scopus 로고
    • Analysis of nuclear-cytoplasmic export of mRNA expressed from transfected cDNAs using riboprobes for fluorescence in situ hybridization
    • White, K. N., and L. Fu. 1995. Analysis of nuclear-cytoplasmic export of mRNA expressed from transfected cDNAs using riboprobes for fluorescence in situ hybridization. Methods Mol. Cell. Biol. 5:222.
    • (1995) Methods Mol. Cell. Biol. , vol.5 , pp. 222
    • White, K.N.1    Fu, L.2
  • 33
    • 0016428323 scopus 로고
    • Identification and some properties of rat secretory component
    • Vaerman, J. P., J. F. Heremans, H. Bazin, and A. Beckers. 1975. Identification and some properties of rat secretory component. J. Immunol. 114:265.
    • (1975) J. Immunol. , vol.114 , pp. 265
    • Vaerman, J.P.1    Heremans, J.F.2    Bazin, H.3    Beckers, A.4
  • 34
    • 0027462711 scopus 로고
    • Monitoring foreign gene expression by a human adenovirus-based vector using firefly luciferase gene as a reporter
    • Mittal, S. K., M. R. McDermott, D. C. Johnson, L. Prevec, and F. L. Graham. 1993. Monitoring foreign gene expression by a human adenovirus-based vector using firefly luciferase gene as a reporter. Virus Res. 28:67.
    • (1993) Virus Res. , vol.28 , pp. 67
    • Mittal, S.K.1    McDermott, M.R.2    Johnson, D.C.3    Prevec, L.4    Graham, F.L.5
  • 35
    • 0025114152 scopus 로고
    • Vaccination and protection from a lethal virus: Identification, incorporation and use of a cytotoxic T-lymphocyte giycoprotein epitope
    • Klavinskis, L. S., J. L. Whitton, E. Joly, and M. B. A. Oldstone. 1990. Vaccination and protection from a lethal virus: identification, incorporation and use of a cytotoxic T-lymphocyte giycoprotein epitope. Virology 178:393.
    • (1990) Virology , vol.178 , pp. 393
    • Klavinskis, L.S.1    Whitton, J.L.2    Joly, E.3    Oldstone, M.B.A.4
  • 36
    • 0023968084 scopus 로고
    • Expression of the firefly luciferase gene in vaccinia virus: A highly sensitive gene marker to follow virus dissemination in tissues of infected animals
    • Rodriguez, J. F., D. Rodriguez, J. R. Rodriguez, E. B. McGowan, and M. Esteban. 1988. Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals. Proc. Natl. Acad. Sci. USA 85:1667.
    • (1988) Proc. Natl. Acad. Sci. USA , vol.85 , pp. 1667
    • Rodriguez, J.F.1    Rodriguez, D.2    Rodriguez, J.R.3    McGowan, E.B.4    Esteban, M.5
  • 37
    • 0023870943 scopus 로고
    • Immunobarriers of the mucosa of the upper respiratory and digestive pathways
    • Brandtzaeg, P. 1988. Immunobarriers of the mucosa of the upper respiratory and digestive pathways. Acta Otolaryngol. (Stockh.) 105:172.
    • (1988) Acta Otolaryngol. (Stockh.) , vol.105 , pp. 172
    • Brandtzaeg, P.1
  • 40
    • 25744466637 scopus 로고    scopus 로고
    • Mucosal phenotype of antiviral cytotoxic T lymphocytes in the vaginal mucosa of SIV-infected rhesus macaques
    • McChesney, M. B., J. R. Collins, and C. J. Miller. 1997 Mucosal phenotype of antiviral cytotoxic T lymphocytes in the vaginal mucosa of SIV-infected rhesus macaques. AIDS Res. Hum. Retroviruses 23(Suppl 1):S1.
    • (1997) AIDS Res. Hum. Retroviruses , vol.23 , Issue.SUPPL. 1
    • McChesney, M.B.1    Collins, J.R.2    Miller, C.J.3
  • 41
    • 0029809510 scopus 로고    scopus 로고
    • Long lived cytotoxic T lymphocyte memory in mucosal tissues after mucosal but not systemic immunisation
    • Gallichian, W. S., and K. L. Rosenthal. 1996. Long lived cytotoxic T lymphocyte memory in mucosal tissues after mucosal but not systemic immunisation. J. Exp. Med. 184:1879.
    • (1996) J. Exp. Med. , vol.184 , pp. 1879
    • Gallichian, W.S.1    Rosenthal, K.L.2
  • 42
    • 0029825440 scopus 로고    scopus 로고
    • Intranasal immunization induces long term protection in mice against a Chlamydia trachomatis genital challenge
    • Pal, S., E. M. Peterson, and L. M. de la Maza. 1996. Intranasal immunization induces long term protection in mice against a Chlamydia trachomatis genital challenge. Infect. Immun. 64:5341.
    • (1996) Infect. Immun. , vol.64 , pp. 5341
    • Pal, S.1    Peterson, E.M.2    De La Maza, L.M.3
  • 44
    • 0025265502 scopus 로고
    • Secretory immune responses in mouse vaginal fluid after pelvic, parenteral or vaginal immunisation
    • Thapar, M. A., E. L. Parr, and M. B. Parr. 1990. Secretory immune responses in mouse vaginal fluid after pelvic, parenteral or vaginal immunisation. Immunology 70:121.
    • (1990) Immunology , vol.70 , pp. 121
    • Thapar, M.A.1    Parr, E.L.2    Parr, M.B.3
  • 46
    • 0002951357 scopus 로고
    • Mucosal immunity in the female and male reproductive tracts
    • P. L. Ogra, J. Mesteky, M. E. Lamm, J. R. Strober, J. R. McGhee, and J. Bienenstock, eds. Academic Press, San Diego
    • Parr, M. B., and E. L. Parr. 1994. Mucosal immunity in the female and male reproductive tracts. In Handbook of Mucosal Immunology. P. L. Ogra, J. Mesteky, M. E. Lamm, J. R. Strober, J. R. McGhee, and J. Bienenstock, eds. Academic Press, San Diego, p. 667.
    • (1994) Handbook of Mucosal Immunology , pp. 667
    • Parr, M.B.1    Parr, E.L.2
  • 47
    • 0025319977 scopus 로고
    • Antigen recognition in the female reproductive tract. I. Uptake of intraluminal tracers in the mouse vagina
    • Parr, M. B., and E. L. Parr. 1990. Antigen recognition in the female reproductive tract. I. Uptake of intraluminal tracers in the mouse vagina. J. Reprod. Immunol. 17:101.
    • (1990) J. Reprod. Immunol. , vol.17 , pp. 101
    • Parr, M.B.1    Parr, E.L.2
  • 52
    • 0025038703 scopus 로고
    • Ultrastructural study of liposome uptake by M cells of rat Peyer's patch: An oral vaccine oral vaccine system for delivery of purified antigen
    • Childers, N. K., D. R. Francis, N. F. McGhee, and S. M. Michalek. 1990. Ultrastructural study of liposome uptake by M cells of rat Peyer's patch: an oral vaccine oral vaccine system for delivery of purified antigen. Reg. Immunol. 3:8.
    • (1990) Reg. Immunol. , vol.3 , pp. 8
    • Childers, N.K.1    Francis, D.R.2    McGhee, N.F.3    Michalek, S.M.4
  • 53
    • 0025554371 scopus 로고
    • Mucosal and systemic responses to an oral liposome-Streptococcus mutans carbohydrate vaccine in humans
    • Childers, N. K., S. M. Michalek., D. G. Pritchard, and J. R. McGhee. 1991. Mucosal and systemic responses to an oral liposome-Streptococcus mutans carbohydrate vaccine in humans. Reg. Immunol. 3:289.
    • (1991) Reg. Immunol. , vol.3 , pp. 289
    • Childers, N.K.1    Michalek, S.M.2    Pritchard, D.G.3    McGhee, J.R.4
  • 54
    • 0031252387 scopus 로고    scopus 로고
    • Intranasal immunization of a DNA vaccine with IL-12 and granulocyte-macrophage colony-stimulating factor (GM-CSF) expressing plasmids in liposome induces strong mucosal and cell-mediated immune responses against HIV-1 antigens
    • Okada, E., S. Sasaki, N. Ishii, I. Aoki, T. Yasuda, K. Nishioka, J. Fukushima, J.-I. Miyazaki, B. Wahren, and K. Okuda. 1997. Intranasal immunization of a DNA vaccine with IL-12 and granulocyte-macrophage colony-stimulating factor (GM-CSF) expressing plasmids in liposome induces strong mucosal and cell-mediated immune responses against HIV-1 antigens. J. Immunol. 159:3638.
    • (1997) J. Immunol. , vol.159 , pp. 3638
    • Okada, E.1    Sasaki, S.2    Ishii, N.3    Aoki, I.4    Yasuda, T.5    Nishioka, K.6    Fukushima, J.7    Miyazaki, J.-I.8    Wahren, B.9    Okuda, K.10
  • 56
    • 0030932988 scopus 로고    scopus 로고
    • Induction of mucosal immunity against herpes simplex virus by plasmid DNA immunization
    • Kuklin, N., M. Daheshia, K. Karem, E. Manickan, and B. T. Rouse. 1997. Induction of mucosal immunity against herpes simplex virus by plasmid DNA immunization. J. Virol. 71:3138.
    • (1997) J. Virol. , vol.71 , pp. 3138
    • Kuklin, N.1    Daheshia, M.2    Karem, K.3    Manickan, E.4    Rouse, B.T.5
  • 57
    • 15844394511 scopus 로고    scopus 로고
    • Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques
    • Lehner, T., Y. Wang, M. Cranage, L. A. Bergmeier, E. Mitchell, L. S. Klavinskis, L. Tao, G. Hall, M. Dennis, R. Brookes, et al. 1996. Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques. Nat. Med. 2:767.
    • (1996) Nat. Med. , vol.2 , pp. 767
    • Lehner, T.1    Wang, Y.2    Cranage, M.3    Bergmeier, L.A.4    Mitchell, E.5    Klavinskis, L.S.6    Tao, L.7    Hall, G.8    Dennis, M.9    Brookes, R.10
  • 59
    • 0030000550 scopus 로고    scopus 로고
    • Antigen sampling across epithelial barriers and induction of mucosal immune responses
    • Neutra, M. R., E. Pringault, and J.-P. Kraehenbuhl. 1996. Antigen sampling across epithelial barriers and induction of mucosal immune responses. Annu. Rev. Immunol. 14:275.
    • (1996) Annu. Rev. Immunol. , vol.14 , pp. 275
    • Neutra, M.R.1    Pringault, E.2    Kraehenbuhl, J.-P.3
  • 60
    • 0021850386 scopus 로고
    • Induction of IgE-secreting cells and IgE isotype specific suppressor T cells in the respiratory lymph nodes of rats in response to antigen
    • Sedgwick, J. D., and P. G Holt. 1985. Induction of IgE-secreting cells and IgE isotype specific suppressor T cells in the respiratory lymph nodes of rats in response to antigen. Cell. Immunol. 94:182.
    • (1985) Cell. Immunol. , vol.94 , pp. 182
    • Sedgwick, J.D.1    Holt, P.G.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.