Quantitative evaluation of preparation procedures affecting immunogold staining in post embedding immunocytochemistry

Acta Histochemica et Cytochemica

Volumn 30, Issue 5-6, 1997, Pages 497-504

  Yokota, Sadaki ^a   Okada, Yoshiie ^a  

^a UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

Antigenicity; Dehydration; Embedding resins; Glutaraldehyde; Polymerization; Primary antibody reaction; Quantitative analysis

Indexed keywords

EID: 0031427568     PISSN: 00445991     EISSN: None     Source Type: Journal    
DOI: 10.1267/ahc.30.497     Document Type: Article

References (25)

1. Acetarin, J. -D., Carlemalm, E., Kellenberger, E. and Villiger, W.: Correlation of some mechanical properties of embedding resins with their behavior in microtomy. J. Electron Microsc. Tech. 6; 63-79, 1987.
2. Bendayan, M.: Protein A-gold electron microscopic immunocytochemistry: Methods, applications, and limitation. J. Electron Microsc. Tech. 1; 243-270, 1984.
3. Bendayan, M., Roth, J., Perrele, A. and Orci, L.: Quantitative immuncytochemical localization of pancreatic secretory proteins in subcellular compartments of the rat acinar cell. J. Histochem. Cytochem. 28; 149-160, 1982.
4. Berod, A., Hartman, B. K. and Pujol, J. F.: Importance of fixation in immunohistochemistry: Use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase. J. Histochem. Cytochem. 29; 844-850, 1981.
5. Bowes, J. H. and Carter, C. W.: The reaction of glutaraldehyde with proteins and other biological materials. J. Roy. Microsc. Soc. 85; 193-200, 1966.
6. Carlemalm, E., Garavito, R. M. and Villiger, W.: Resin development for electron microscopy and an analysis of embedding at low temperature. J. Microsc. 126; 123-143, 1982.
7. Craig, S. and Goodchild, D. J.: Postembedding immunolabeling. Some effects of tissue preparation on the antigenicity of plant proteins. Eur. J. Cell Biol. 28; 251-256, 1982.
8. Craig, S. and Miller, C.: LR White resin and improved on-grid immunogold detection of vicilin, a pea seed storage protein. Cell Biol. Int. Rep. 8; 879-886, 1984.
9. De Roe, C., Courtoy, P. J. and Baudhuin, P.: A model of protein-colloidal gold interactions. J. Histochem. Cytochem. 35; 1191-1198, 1987.
10. Griffiths, G.: Fixation for fine structure preservation and immunocytochemistry. In "Fine Structure Immunocyto-chemistry" Springer-Verlag, Berlin, 1993, pp. 26-89.
11. Kellenberger, E., Dürrenberger, M., Villiger, W., Carlemalm, E. and Wurtz, M.: The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J. Histochem. Cytochem. 35; 959-969, 1987.
12. Miyazawa, S., Osumi, T. and Hashimoto, T.: The presence of a new 3-oxoacyl-CoA thiolase in rat liver peroxisomes. Eur. J. Biochem. 103; 589-596, 1980.
13. Okada, Y. and Wakabayashi, K.: Purification and characterization of esterase D-1 and D-2 from human erythrocytes. Arch. Biochem. Biophys. 263; 130-136, 1988.
14. Osumi, T. and Hashimoto, T.: Purification and properties of mitochondrial and peroxisomal 3-hydroxyacyl-CoA dehydrogenase from rat liver. Arch. Biochem. Biophys. 203; 372-383, 1984.
15. Rasmussen, K. E. and Albrechtsen, J.: Glutaraldehyde. The influence of pH, temperature, and buffering on the polymerization rate. Histochemistry 38; 19-26, 1974.
16. Richards, F. M. and Knowles, J. R.: Glutaraldehyde as a protein crosslinking reagent. J. Mol. Biol. 37; 231-233, 1968.
17. Roth, J.: The protein A-gold (pAg) technique. A quantitative and qualitative approach for antigen localization on thin sections. In "Techniques in Immunocytochemistry" vol. 1., ed. by G. R. Bullock and P. Petrusz, Academic Press, New York, 1982, pp. 108-133.
18. Roth, J.: Post-embedding cytochemistry with gold labelled reagents, a review. J. Microsc. 143; 125-137, 1986.
19. Roth, J., Bendayan, M., Carlemalm, E., Villiger, W. and Garavito, M.: Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J. Histochem. Cytochem. 29; 663-671, 1981.
20. Yamamoto, A. and Tashiro, Y.: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J. Histochem. Cytochem. 42; 1463-1470, 1994.
21. Yokota, S.: Cytochemical and immunocytochemical study on the peroxisomes of rat liver after administration of a hypolipidemic drug, MLM-160. Eur. J. Cell Biol. 53; 112-121, 1990.
22. Yokota, S. and Fahimi, H. D.: Immunocytochemical localization of catalase in rat liver. J. Histochem. Cytochem. 29; 805-812, 1981.
23. Yokota, S. and Fahimi, H. D.: Immunoelectron microscopic localization of acyl-CoA oxidase in rat liver and kidney. Ann. N.Y. Acad. Sci. 386; 491-494, 1982.
24. Yokota, S., Oda, T. and Ichiyama, A.: Immunocytochemical localization of serine : pyruvate aminotransferase in peroxisomes of the monkey liver. Yamanashi Med. J. 3; 89-96, 1988.
25. Yoshimura, N., Murachi, T., Heath, R., Kay, J. J., Jasani, B. and Newman, G. R.: Immunogold electron microscopic localization of calpain I in skeletal muscle of rats. Cell Tissue Res. 244; 265-270, 1986.