Identification and characterization of an Escherichia coli invasion gene locus, ibeB, required for penetration of brain microvascular endothelial cells

Infection and Immunity

Volumn 67, Issue 5, 1999, Pages 2103-2109

  Huang, Sheng He ^a,b   Chen, Yu Hua ^a   Fu, Qi ^a   Stins, Monique ^a   Wang, Ying ^a   Wass, Carol ^a   Kim, Kwang Sik ^a,b  

^a CHILDREN S HOSPITAL LOS ANGELES   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BLOOD BRAIN BARRIER; BRAIN BLOOD VESSEL; ESCHERICHIA COLI; MENINGITIS; MICROVASCULATURE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

EID: 0032903979     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.67.5.2103-2109.1999     Document Type: Article

References (25)

1. Blattner, F. R., G. Plunkett III, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, J. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1462.
2. de Vries, F. P., A. van Der Ende, J. P. van Putten, and J. Dankert. 1996. Invasion of primary nasopharyngeal epithelial cells by Neisseria meningitidis is controlled by phase variation of multiple surface antigens. Infect. Immun. 64:2998-3006.
3. Donnenberg, M. S., S. B. Calderwood, A. Donohue-Rolfe, G. T. Keusch, and J. B. Kaper. 1990. Construction and analysis of TnphoA mutants of enteropathogenic Escherichia coli that invade HEp-2 cells. Infect. Immun. 58:1565-1571.
4. Donnenberg, M. S., and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59:4310-4317.
5. Donnenberg, M. S., J. Yu, and J. B. Kaper. 1993. A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. J. Bacteriol. 175:4670-4680.
6. Hartman, A. B., M. Venkatesan, E. V. Oaks, and J. M. Buysse. 1990. Sequence and molecular characterization of a multicopy invasion plasmid antigen gene, ipaH, of Shigella flexneri. J. Bacteriol. 172:1905-1915.
7. Huang, S. H., C. A. Wass, Q. Fu, N. A. Prasadarao, M. F. Stins, and K. S. Kim. 1995. Escherichia coli invasion of brain microvascular endothelial cells in vitro and in vivo: molecular cloning and characterization of invasion gene ibe10. Infect. Immun. 63:4470-4475.
8. Huang, S. H. 1997. Inverse polymerase chain reaction: application in cDNA cloning. Methods Mol. Biol. 67:287-294.
9. Huang, S. H., A. Tang, B. Drisco, S.-Q. Zhang, R. Seeger, C. Ching Li, and A. Y. Jong. 1994. Human dTMP kinase: gene expression and enzyme activity coinciding with cell cycle progression and cell growth. DNA Cell Biol. 13: 461-471.
10. Kim, K. S., H. Itabashi, P. Gemski, R. L. Warren, and A. S. Cross. 1992. The K1 capsule is the critical determinant in the development of E. coli meningitis in the rat. J. Clin. Investig. 90:897-905.
11. Kolmodin, L. A., and J. F. Williams. 1997. PCR: basic principles and routine practice. Methods Mol. Biol. 67:1-15.
12. Manoil, C., and J. Beckwith. 1985. TnphoA: a transposon probe for protein export signals. Proc. Natl. Acad. Sci. USA 82:8129-8133.
13. - gonococci and meningococci to epithelial cells elicits cortical actin rearrangements and clustering of tyrosine-phosphorylated proteins. Infect. Immun. 65: 4341-4349.
14. Nizet, V., K. S. Kim, M. Stins, M. Jonas, E. Y. Chi, D. Nguyen, and C. E. Rubens. 1997. Invasion of brain microvascular endothelial cells by group B streptococci. Infect. Immun. 65:5074-5081.
15. Prasadarao, N. V., C. A. Wass, J. N. Weiser, M. F. Stins, S. H. Huang, and K. S. Kim. 1996. Outer membrane protein A of Escherichia coli contributes to invasion of brain microvascular endothelial cells. Infect. Immun. 64:146-151.
16. Quagliarello, V., and W. M. Scheld. 1992. Bacterial meningitis: pathogenesis, pathophysiology, and progress. N. Engl. J. Med. 327:864-872.
17. Ring, A., J. N. Weiser, and E. I. Tuomanen. 1998. Pneumococcal trafficking across the blood-brain barrier: molecular analysis of a novel bidirectional pathway. J. Clin. Investig. 102:347-360.
18. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
19. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
20. Sankar, P., M. E. Hutton, R. A. VanBogelen, R. L. Clark, and F. C. Neidhardt. 1993. Expression analysis of cloned chromosomal segments of Escherichia coli. J. Bacteriol. 175:5145-5152.
21. Silver, R. P., W. Aaronson, A. Sutton, and R. Schneerson. 1980. Comparative analysis of plasmids and some metabolic characteristics of Escherichia coli K1 from diseased and healthy individuals. Infect. Immun. 29:200-206.
22. Stins, M. F., N. V. Prasadarao, L. Ibric, C. A. Wass, P. Luckett, and S. K. Kim. 1994. Binding characteristics of S fimbriated Escherichia coli to isolated brain microvascular endothelial cells. Am. J. Pathol. 145:1228-1236.
23. Stins, M. F., F. Gilles, and K. S. Kim. 1997. Selective expression of adhesion molecules on human brain microvascular endothelial cells. J. Neuroimmunol. 76:81-90.
24. Tuomanen, E. 1996. Entry of pathogens into the central nervous system. FEMS Microbiol. Rev. 18:289-299.
25. Virji, M., K. Makepeace, D. J. Ferguson, M. Achtman, and E. R. Moxon. 1993. Meningococcal Opa and Ope proteins: their role in colonization and invasion of human epithelial and endothelial cells. Mol. Microbiol. 10:499-510.